In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep

Abstract

Aim:The aim of the study was to evaluate the antibacterial effects of zinc oxide nanoparticles (ZnO-NPs) and its possible alternative use for the treatment for mastitis in sheep and to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ZnO-NPs against multidrug-resistant Staphylococcus aureus and Escherichia coli strains isolated from subclinical mastitis cases in sheep.Materials and Methods:A total of 50 pooled milk samples were collected from ewes with subclinical mastitis. Milk samples were cultured using standard laboratory techniques, and multidrug-resistant bacterial strains were determined using the Kirby–Bauer disk diffusion method. The MIC and MBC of ZnO-NPs were determined against isolated multidrug-resistant S. aureus and E. coli strains using microwell dilution method.Results:A total of 43 different bacterial isolates were recovered from milk samples of ewes affected with subclinical mastitis. Isolated strains of S. aureus and E. coli were found resistant to three or more common antibacterial agents and were used to determine the MIC and MBC of ZnO-NPs. The MIC and MBC values of ZnO-NPs were significantly lower for S. aureus than that for E. coli. The MIC and MBC of ZnO-NPs against S. aureus were 3.9 µg/ml and 7.81 µg/ml, respectively, while for E. coli, the MIC and MBC of ZnO-NPs were 31.25 µg/ml and 62.5 µg/ml, respectively.Conclusion:Results of this study indicate the potential antibacterial effects of ZnO-NPs against multidrug-resistant S. aureus and E. coli isolated from ovine subclinical mastitis at concentrations of 3.9 µg/ml and 31.25 µg/ml, respectively.

Structural and biochemical modifications of model and native membranes of human immune cells in response to the action of zinc oxide nanoparticles

“…Effective antibacterial action also requires high doses of these nanomaterials. Many studies indicate that the lowest concentration of ZnO nanoparticle suspension, which caused an over 99.9% reduction in initial inoculum (MBC) ranges from tens to even 5000 mg/L depending on the bacterial serotype and the method of nanoparticle synthesis (Alekish, Ismail, Albiss, & Nawasrah, 2018; Hoseinzadeh, Alikhani, Samarghandi, & Shirzad‐Siboni, 2013; Raj, Lawrence, Jalees, & Lawrence, 2015; Souza, Haberbeck, Riella, Ribeiro, & Carciofi, 2019). Despite high contact concentrations, the concentration of ZnO‐NPs in the body will be lower, and therefore, the amounts used for the presented research were much lower than usually used in industry.…”

Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

“…Klebsiella pneumoniae is an important pathogen that is involved in respiratory and urinary tract infections of hospitalized patients. It exists as part of the normal flora of the human respiratory tract and intestines and is isolated from the oropharyngeal cavity at a rate of 1–6% [ 5 ]. However, it also causes septicemia and iatrogenic infections.…”

“…However, it also causes septicemia and iatrogenic infections. Moreover, it is associated with long-term hospitalized patients and those who have been in an intensive care unit (ICU) [ 5 ]. K. pneumoniae is resistant to ampicillin and carbenicillin and recently became resistant to cephalosporins, cotrimoxazole, aminoglycosides, and imipenem [ 5 ].…”

Histopathological changes after treatment of Mycoplasma bovis infected Does with Zinc oxide nanoparticles as a new tool.

“…5 & 6 a &b) demonstrated enhancing influence of ZnO nanoparticles on the activity of lincospectin against M. bovis. ZnO-NPs also were causing destructive oxidative stress to bacterial cells and disrupt the metabolic activity of bacterial cells and therefore inhibit their growth [29].…”