In vitro antifungal activity and mechanism of essential oil from fennel (Foeniculum vulgare L.) on dermatophyte species

Zeng, Hong; Chen, Xinping; Liang, Jingnan

doi:10.1099/jmm.0.077768-0

Cited by 73 publications

(35 citation statements)

References 38 publications

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“…Previous studies have shown a strong antagonistic activity of thyme essential oil against undesirable bacteria (Hosseini et al, 2009). However, it is shown that this is not the case with the fungus evaluated here, which could be explained due to when the thyme essential oil is mixed with Tween ® , then the antifungal capacity decreases significantly as was previously reported (Zeng et al, 2015).…”

Section: Effect Of Essential Oils On the In Vitro Growth Of Colletotrmentioning

confidence: 48%

Chitosan composite films: physicochemical characterization and their use as coating in papaya Maradol stored at room temperature

et al. 2017

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The main objectives of this study were; first formulate and evaluate some properties of chitosan composite films enriched with essential oils of cinnamon, thyme, clove, and lime alone or in combination; and second select the most appropriate formulation and evaluate their effect on physiological properties and fungal incidence of papaya Maradol stored at environmental tropical conditions. Breaking strength, thickness, color, moisture, water vapor permeability, solubility and water sorption of films were measured. Furthermore, in vitro activity was evaluated against Colletotrichum gloeosporioides Penz. Using multivariate analysis and based on the most desirable characteristics (low water sorption values, water vapor permeability, solubility, and greater inhibition of C. gloeosporioides), three formulations of films were used lately for coated fruit of papaya Maradol and stored at 28 ± 2 °C and 85 % RH. Two control treatments were included: one uncoated and other treated with Mancozeb® fungicide. At six days of storage, the fruit of both control treatments showed evident signs of ripening; contrary to the treatments wherein fruit were coated, and had low respiration, other attributes as firmness, little change in the external color of the fruit were manifested. Similarly, the use of composite films reduced the fungus C. gloeosporioides by 30-50 %. The use of film enriched with essential oil of 1 % cinnamon- 1 % thyme improved the characteristics of fruit quality, increased shelf life and reduced by 50 % the incidence of fungus in papaya Maradol stored under humid tropic environmental conditions. This coating can be an alternative to potentially reduce the need for cold storage during postharvest handling.

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Section: Effect Of Essential Oils On the In Vitro Growth Of Colletotrmentioning

confidence: 48%

Chitosan composite films: physicochemical characterization and their use as coating in papaya Maradol stored at room temperature

et al. 2017

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“…The cells were dried at 60°C for 6 h and then at 40°C overnight, and weighed. Percent growth inhibition on the basis of dry weight was calculated as follows: (Control weight-Sample weight)/ Control weight × 100 [27]. All experiments were conducted in triplicate.…”

Section: Determination Of Biomassmentioning

confidence: 99%

“…Membrane damage was examined following the procedure described previously but with a slight modification [27]. Briefly, a single C. neoformans colony from an overnight SDA culture plate was inoculated into 60 mL of SDB containing different concentrations of the EO.…”

Section: Determination Of Ergosterol Content In the Plasma Membrane Omentioning

confidence: 99%

Anti-cryptococcus Activity and Mechanism of Essential Oil from Coreopsis tinctoria Flowering on Cryptococcus

Zeng¹,

Li²,

Ding³

et al. 2016

Fungal Genom Biol

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The essential oil (EO) extracted from the flower of Coreopsis tinctoria Nutt. is a kind of plant-derived natural therapeutics against Cryptococcus neoformans. In our study, the anti-cryptococcal effects of the EO were observed from various aspects, such as minimal inhibitory concentration, minimum fungicidal concentration, germ tube formation, and biomass. The results indicated that the EO had potent antifungal activities on C. neoformans and that it was more effective than the commonly used antifungal agents fluconazole and amphotericin B. To further elucidate the mechanism of the antifungal action, the effect of the EO on the plasma membrane and the mitochondria of C. neoformans were investigated. The lesion in the plasma membrane was detected through flow cytometry and was further verified through the inhibition of ergosterol synthesis. The essential oil caused morphological changes in the cells of C. neoformans and a reduction in the ergosterol quantity. Moreover, mitochondrial membrane potential, mitochondrial ATPase, succinate dehydrogenase (SDH), and malate dehydrogenase (MDH) were detected. The reactive oxygen species (ROS) accumulation was examined through fluorometric assay. Decreased SDH, MDH, and ATPase in C. neoformans cells were also observed in a dose-dependent manner. The above dysfunctions of the mitochondria caused ROS accumulation in C. neoformans. A reduction in cell viability was prevented through the addition of L-cysteine, and this reduction indicates that ROS is an important mediator of the antifungal action of the EO. In summary, EO from C. tinctoria flower showed anti-cryptococcus activity. Its mechanism of action is to disrupt the permeability barrier of the plasma membrane and the mitochondrial dysfunction-induced ROS accumulation in C. neoformans. With better antifungal activity than the commonly used antifungal agents and less possibility to induce drug resistance, the EO can be used as a potential anti-cryptococcal agent.

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“…Throughout our study results, we found inhibition zone only against T. mentagrophytes and M. canis that might be more sensitive to fennel seeds essential oil than those of the other tested dermophytes. Zeng et al [37] showed that fennel seed essential oil could be used as a potential antidermatophytic agent. Ozcan et al [38] also reported that, the most affected fungus by the bitter fennel oil rich in estragole was Alternaria alternata followed by Fusarium oxysporum.…”

Section: Antifungal Activitymentioning

confidence: 99%

Chemical Composition and Antifungal Activity of Foeniculum vulgare Mill

et al. 2020

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The essential oils of plants have been widely recognized in the pharmaceutical industry due to their various benefits as well as their biological activities. In Algeria, the seeds of Foeniculum vulgare Mill (Family Apiaceae) are widely utilized in herbal medicine. We aimed to examine the chemical composition of seed essential oil of this species, and to reveal its in vitro antifungal activity against Candida albicans, Aspergillus flavus, Aspergillus niger, Rhizopus sp., Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton violaceum, Microsporumgypseum, Microsporumcanis and Rhodotorula sp. Chemical analysis of essential oil isolated by hydro-distillation was carried out by GC-FID and GC-MS. The basic components of the essential oil were estragole (84.8%), limonene (7.8%), fenchone (3.1%) and α-pinene (1.3%). The essential oil of F. vulgare revealed higher antifungal activities particularly against C. albicans and Aspergillus species with MIC values of 0.16-0.2 mg/ml.

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In vitro antifungal activity and mechanism of essential oil from fennel (Foeniculum vulgare L.) on dermatophyte species

Cited by 73 publications

References 38 publications

Chitosan composite films: physicochemical characterization and their use as coating in papaya Maradol stored at room temperature

Chitosan composite films: physicochemical characterization and their use as coating in papaya Maradol stored at room temperature

Anti-cryptococcus Activity and Mechanism of Essential Oil from Coreopsis tinctoria Flowering on Cryptococcus

Chemical Composition and Antifungal Activity of Foeniculum vulgare Mill

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