In vitro Antifungal Activity and Mechanism of Action of Tea Polyphenols and Tea Saponin against &lt;b&gt;&lt;i&gt;Rhizopus stolonifer&lt;/i&gt;&lt;/b&gt;

Jiang, Xiaodong; Feng, Kejue; Yang, Xiaoping

doi:10.1159/000430866

Cited by 24 publications

(31 citation statements)

References 26 publications

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“…SEM examinations could reveal that rough surface and shrinkage of cells were apparent in the treated hyphae (Tao et al 2014). SEM observations also confirmed the damage that the treated hypha demonstrated bending, entwining, shriveling, collapse and disorganization, and the breakage of the hypha was clearly visible (Jiang et al 2015). In the present experiment, B. cinerea mycelia were treated with Values are expressed as mean ± standard deviation (n = 3).…”

Section: Discussionsupporting

confidence: 79%

Antifungal activity of thymol and carvacrol against postharvest pathogens Botrytis cinerea

Zhang

et al. 2019

J Food Sci Technol

118

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Botrytis cinerea is a primary pathogen causing stem and fruit rot during pre-and post-harvest. In the present study, the main purpose was to inquire into the antifungal activity and potential mechanisms of thymol and carvacrol against B. cinerea. During the experiment, the effects of thymol and carvacrol on physical and biochemical parameters of B. cinerea were evaluated. Results indicated that thymol and carvacrol exhibited strong antifungal activity against the targeted pathogen, with minimum inhibitory concentration and minimum fungicidal concentration of 65 mg/L and 100 mg/L for thymol, and 120 lL/L and 140 lL/L for carvacrol. Thymol and carvacrol changed obviously the morphology of B. cinerea hyphae by disrupting and distorting the mycelia through scanning electron microscopy. The membrane permeability of B. cinerea hyphae was prompted with the increment of two chemical agents' concentration, as evidenced by extracellular conductivity increase, the release of cell constituent, and the decrease of extracellular pH. Furthermore, a marked decline in total lipid content of B. cinerea cells was induced by the two chemical agents, suggesting that the cell membrane structures were destructed. Therefore, present results indicated that thymol and carvacrol may be used as a good alternative to conventional fungicides against B. cinerea in controlling grey molds in horticultural products.

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Section: Discussionsupporting

confidence: 79%

Antifungal activity of thymol and carvacrol against postharvest pathogens Botrytis cinerea

Zhang

et al. 2019

J Food Sci Technol

118

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“…The saponins have been found to have numerous pharmacological effects including anti-fungal [ 29 ], anti-viral [ 30 ], anti-inflammatory [ 31 ], anti-hyperlipidemic [ 32 ], immunomodulatory [ 33 ] and anti-tumor activities [ 17 , 19 ]. Among these, TSE 1 was proved to have higher bioactivity than other saponin monomers present in tea seeds [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

et al. 2018

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Ovarian cancer has the highest mortality rate of all gynecological malignancies and the five-year death rate of patients has remained high in the past five decades. Recently, with the rise of cancer stem cells (CSCs) theory, an increasing amount of research has suggested that CSCs give rise to tumor recurrence and metastasis. Theasaponin E1 (TSE1), which was isolated from green tea (Camellia sinensis) seeds, has been proposed to be an effective compound for tumor treatment. However, studies on whether TSE1 takes effect through CSCs have rarely been reported. In this paper, ALDH-positive (ALDH+) ovarian cancer stem-like cells from two platinum-resistant ovarian cancer cell lines A2780/CP70 and OVCAR-3 were used to study the anti-proliferation effect of TSE1 on CSCs. The ALDH+ cells showed significantly stronger sphere forming vitality and stronger cell migration capability. In addition, the stemness marker proteins CD44, Oct-4, Nanog, as well as Bcl-2 and MMP-9 expression levels of ALDH+ cells were upregulated compared with the original tumor cells, indicating that they have certain stem cell characteristics. At the same time, the results showed that TSE1 could inhibit cell proliferation and suspension sphere formation in ALDH+ cells. Our data suggests that TSE1 as a natural compound has the potential to reduce human ovarian cancer mortality. However, more research is still needed to find out the molecular mechanism of TSE1-mediated inhibition of ALDH+ cells and possible drug applications on the disease.

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“…TS has been demonstrated as being an excellent bio-surfactant with numerous pharmacological activities. TS has been documented as having gastroprotective [ 33 ], anti-fungal [ 34 ], anti-viral [ 25 ], anti-oxidative [ 24 ], anti-obesity [ 35 ], anti-inflammatory [ 24 , 28 ], anti-hyperglycemic [ 27 ], immunomodulatory [ 36 ], and anti-tumor [ 35 , 37 , 38 ] tendencies. However, unlike other phenolic compounds, EGCG [ 39 , 40 , 41 ] and TFs [ 37 , 42 ] separated from tea plants, TS has not been reported as having pharmacological influences on human ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

Jia

Gao

et al. 2017

Molecules

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Ovarian cancer is regarded as one of the most severe malignancies for women in the world. Death rates have remained steady over the past five decades, due to the undeniable inefficiency of the current treatment in preventing its recurrence and death. The development of new effective alternative agents for ovarian cancer treatment is becoming increasingly critical. Tea saponins (TS) are triterpenoidsaponins composed of sapogenins, glycosides, and organic acids, which possess a variety of pharmacological activities, and have shown promise in the anti-cancer field. Through cell CellTiter 96® Aqueous One Solution Cell Proliferation assay (MTS) assay, colony formation, Hoechst 33342 staining assay, caspase-3/7 activities, flow cytometry for apoptosis analysis, and Western blot, we observed that TS isolated from the seeds of tea plants, Camellia sinensis, exhibited strong anti-proliferation inhibitory effects on OVCAR-3 and A2780/CP70 ovarian cancer cell lines. Our results indicate that TS may selectivity inhibit human ovarian cancer cells by mediating apoptosis through the extrinsic pathway, and initiating anti-angiogenesis via decreased VEGF protein levels in a HIF-1α-dependent pathway. Our data suggests that, in the future, TS could be incorporated into a potential therapeutic agent against human ovarian cancer.

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In vitro Antifungal Activity and Mechanism of Action of Tea Polyphenols and Tea Saponin against <b><i>Rhizopus stolonifer</i></b>

Cited by 24 publications

References 26 publications

Antifungal activity of thymol and carvacrol against postharvest pathogens Botrytis cinerea

Antifungal activity of thymol and carvacrol against postharvest pathogens Botrytis cinerea

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

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