In vitro antifungal activity and possible mechanisms of action of chelerythrine

Wei, Qinghui; Cui, Daizong; Liu, Xuefeng; Chai, Yangyang; Zhao, Na; Wang, Jueyu; Zhao, Min

doi:10.1016/j.pestbp.2020.01.007

Cited by 38 publications

(31 citation statements)

References 24 publications

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“…In this study, UPLC-MS/MS was employed to investigate changes in metabolism in U. virens following treatment with chelerythrine. PCA and OPLS-DA results showed revealed obvious differences between treatment and control groups, and combining differential metabolites and enriched pathways showed that treatment with chelerythrine reduced stress resistance of the pathogen, and eventually led to cell apoptosis, consistent with previous reports (Wei et al, 2020).…”

Section: Discussionsupporting

confidence: 89%

“…We extracted chelerythrine as previously described (Wei et al, 2020). Methyl alcohol, methanoic acid and ammonium acetate were purchased from Thermo Fisher (Shanghai, China), and water was from Merck (Beijing, China).…”

Section: Reagents Media and Strainsmentioning

confidence: 99%

“…Among these, its anti-tumour activity has attracted the most attention, but the mechanism of inhibition is poorly understood, and this is a prerequisite for developing pesticide preparations. Our previous studies showed that chelerythrine inhibits fungus spore germination by up to 86.7%, and it induces apoptosis of U. virens cells (Wei et al, 2020). Developing chelerythrine as a green biological pesticide would be of great signi cance for agricultural production and environmental enhancement.…”

Section: Introductionmentioning

confidence: 99%

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Metabolomics Analysis of the Effects of Chelerythrine on Ustilaginoidea virens

Wei¹,

Shi²,

Li³

et al. 2022

Preprint

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Background: Rice false smut (RFS), caused by Ustilaginoidea virens, is widely distributed in major rice-producing regions. Previous studies showed that treating RFS with chelerythrine can decrease fungus spore germination by 86.7% and induce fungal cell apoptosis. Result: In the present study, the effects of chelerythrine on the metabolism of U. virens were explored using metabolomics, and analysing differentially expressed genes and altered metabolic pathways. A total of 1153 features (740 in positive ion mode, 401 upregulated, 339 downregulated; 413 in negative ionisation mode, 190 upregulated, 223 downregulated). The top 15 metabolites in Random Forest analysis were significantly different between groups. In positive ion mode, purine metabolism (map00230), phenylalanine metabolism (map00360), phenylalanine, tyrosine and tryptophan biosynthesis (map00400), pyrimidine metabolism (map00240) and nitrogen metabolism (map00910) were dominant. Alanine, aspartate and glutamate metabolism (map00250) and phenylalanine metabolism (map00360) were enriched in negative ion mode. Conclusion: Differentially expressed genes and altered metabolic pathways of U. virens were effected by chelerythrine. The findings support future research on the prevention and treatment of RFS by chelerythrine, and provide a theoretical basis for targeted drug delivery.

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Section: Discussionsupporting

confidence: 89%

Section: Reagents Media and Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolomics Analysis of the Effects of Chelerythrine on Ustilaginoidea virens

Wei¹,

Shi²,

Li³

et al. 2022

Preprint

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“…We tested the MIC of each compound and found that only one inhibited germination at the tested concentrations. This compound, chelerythrine, has known broad-spectrum antifungal activity ( 30 , 31 ). While none of the remaining compounds completely inhibited germination at tested concentrations, one compound, the PI3K inhibitor PIK-75 (Z R = 4.9, Fig.…”

Section: Resultsmentioning

confidence: 99%

A Unique Dual-Readout High-Throughput Screening Assay To Identify Antifungal Compounds with Aspergillus fumigatus

Beattie

Krysan

2021

mSphere

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“…In another study, the alkaloid 1-corydalmine suppressed spore germination of A. brassicae and F. udum ( Basha et al, 2007 ; Almadiy and Nenaah, 2018 ). Alkaloids have been found to be capable of destroying cell walls and the membrane structures of fungal cells, thus achieving fungal inhibition ( Moraes et al, 2015 ; Wei et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Changes in the Soil Fungal Community Mediated by a Peganum harmala Allelochemical

Shi

Shao

2022

Front. Microbiol.

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Plants can release phytotoxic allelochemicals into the environment, not only to suppress other plants’ growth, but also alter community structure of soil microbiota, however, the mechanism are often complicated. We designed a consecutive cultivation procedure to evaluate the allelopathic effect of harmaline, the major active allelochemical produced by the desert plant Peganum harmala, on soil microorganisms. Harmaline was added to the soil at 20 μg/g, and after five generations of cultivation, the Chao1, Pielou, Shannon and Simpon indexes changed significantly. In particular, the relative abundances of the dominant fungi, Alternaria sp. and Fusarium sp., declined drastically by 84.90 and 91.90%, respectively. Further in vitro bioassays confirmed that harmaline indeed suppressed growth of 6 Alternaria and Fusarium strains isolated from P. harmala rhizosphere soil. We thus suspect that P. harmala might produce harmaline as an effective carry-on pesticide to defend against general pathogens such as Alternaria sp. and Fusarium sp. and favor itself. Our consecutive cultivation procedure has successfully magnified the core signals from the chaotic data, implying that it can be applied to measure the effects of other allelochemicals on soil microbiota.

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In vitro antifungal activity and possible mechanisms of action of chelerythrine

Cited by 38 publications

References 24 publications

Metabolomics Analysis of the Effects of Chelerythrine on Ustilaginoidea virens

Metabolomics Analysis of the Effects of Chelerythrine on Ustilaginoidea virens

A Unique Dual-Readout High-Throughput Screening Assay To Identify Antifungal Compounds with Aspergillus fumigatus

Changes in the Soil Fungal Community Mediated by a Peganum harmala Allelochemical

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