In vitro Antileishmanial and Antimalarial Activity of Selected Plants of Nepal

Joshi, Bishnu; Hendrickx, Sarah; Magar, Lila Bahadur; Parajuli, Niranjan; Dorny, Pierre; Maes, Louis

doi:10.5455/jice.20160728031236

Cited by 20 publications

(13 citation statements)

References 38 publications

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“…In addition to resistance against existing drugs, no new drugs have reached the market during the last decade (9). Generally, the inability to create efficient vaccines, adverse side-effects of the existing drugs together with the resistance profile indicate the need for development of new antimalarial drugs (10).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Mulisa

Girma

Tesema

et al. 2018

Jundishapur J Nat Pharm Prod

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Background: In addition to resistance against drugs, no new drugs have reached the market during the last decade for malaria treatment. This implies the urgent need to search for new drugs from different sources. Medicinal plants are one of such sources for novel drugs. Therefore, the aim of the current study was to investigate antimalarial activity of crude extract of leaves of Moringa oleifera Lam (Moringaceae) in Plasmodium berghei in a mice model. Methods:The acute toxicity test was used as per standard guidelines. To determine parasite inhibition, 4-day Peter's suppressive test was done. Curative and prophylactic tests were done by applying standard methods. Windows SPSS version 16 was used to analyse the data and analysis of variance (ANOVA) followed by Post Hoc Tukey's HSD to compare results between groups. Results: Death was not observed up to a dose of 2000 mg/kg of the plant extract. In all mice treated with the extract, the level of parasitaemia was significantly reduced in comparison with the control group (P < 0.001) and also a very significant difference was observed in comparison with the chloroquine treated group (P < 0.0001) with lower doses. Mean survival time was significantly increased at all treatment doses. It reduced parasitemia in a dose-dependent manner in both curative and prophylactic activity tests resulting in a significant (P < 0.05) reduction of parasitaemia in comparison with the control group. Conclusions:The result of the current study revealed that the extract possessed significant anti-plasmodial activity as displayed by its ability to suppress P. berghei infection in mice. The present study can be considered as confirmation for traditionally claimed use of the plant against malaria treatment.

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Section: Introductionmentioning

confidence: 99%

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Mulisa

Girma

Tesema

et al. 2018

Jundishapur J Nat Pharm Prod

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“…Moreover, nonselective antileishmanial activity was shown for P. polyphylla, K. pinnata, and Terminalia chebula [63]. Encouraged by these findings, and also by the traditional use of the plants as anthelmintics [63,68], we tested the effects of these plant extracts on the motility of the L4 stage of C. elegans. We used this nonparasitic free-living nematode and model organism to test anthelmintic activity due to its low cost and easy availability of mutant and transgenic strains for functional genomics and proteomics studies as well as to investigate key signaling events and drug interactions and its amenability to laboratory culture methods.…”

Section: Anthelmintic Activitymentioning

confidence: 94%

Section: Anthelmintic Activitymentioning

confidence: 94%

“…We already reported the antiprotozoal activities of crude extracts of select medicinal plants of Nepal and found three active species: Phragmites vallatoria, Ampelocissus tomentosa (for which no antiprotozoal activity had been reported previously), and Terminalia chebula [63]. Moreover, nonselective antileishmanial activity was shown for P. polyphylla, K. pinnata, and Terminalia chebula [63]. Encouraged by these findings, and also by the traditional use of the plants as anthelmintics [63,68], we tested the effects of these plant extracts on the motility of the L4 stage of C. elegans.…”

Section: Anthelmintic Activitymentioning

confidence: 99%

“…Bark, leaves, fruit, or roots of the selected plants (Table 1) were separately collected from different regions in Nepal from December 2013 to April 2014. e rules for plant collection and identification were followed according to the Department of Plant Resources, Ministry of Forest and Environment, Government of Nepal. All the collected plant materials were identified by professional taxonomists at the Department of Plant Resources, and voucher specimens [63] were deposited in the National Herbarium and Plant Laboratories, Department of Plant Resources, Ministry of Forest and Environment, Government of Nepal. e information on these plants with their official taxonomic name (according to the plant list: http://www.theplantlist.org), indigenous use, used part(s), literature availability, and voucher number is shown in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

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Antibacterial, Antifungal, Antiviral, and Anthelmintic Activities of Medicinal Plants of Nepal Selected Based on Ethnobotanical Evidence

Joshi

Panda

Jouneghani

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Infections by microbes (viruses, bacteria, and fungi) and parasites can cause serious diseases in both humans and animals. Heavy use of antimicrobials has created selective pressure and caused resistance to currently available antibiotics, hence the need for finding new and better antibiotics. Natural products, especially from plants, are known for their medicinal properties, including antimicrobial and anthelmintic activities. Geoclimatic variation, together with diversity in ethnomedicinal traditions, has made the Himalayas of Nepal an invaluable repository of traditional medicinal plants. We studied antiviral, antibacterial, antifungal, and anthelmintic activities of medicinal plants, selected based upon ethnobotanical evidence. Methods. Ethanolic and methanolic extracts were tested (1) on a panel of microbes: two Gram-positive bacteria (Staphylococcus aureus and Listeria innocua), four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, and Shigella sonnei), and one fungal species: Candida albicans; (2) against three different viruses: yellow fever, chikungunya, and enterovirus; and (3) on the nematode Caenorhabditis elegans. Also, cytotoxicity was assessed on human hepatoma (Huh), rhabdosarcoma (RD), and Vero (VC) cell lines. Results. Of 18 plants studied, Ampelocissus tomentosa and Aleuritopteris anceps inhibited S. aureus (MIC 35 μg/mL and 649 μg/mL, respectively) and Pseudomonas aeruginosa (MIC 15 μg/mL and 38 μg/mL, respectively). Rhododendron arboreum and Adhatoda vasica inhibited S. enterica (MIC 285 μg/mL and 326 μg/mL, respectively). Kalanchoe pinnata, Ampelocissus tomentosa, and Paris polyphylla were active against chikungunya virus, and Clerodendrum serratum was active against yellow fever virus (EC50 15.9 μg/mL); Terminalia chebula was active against enterovirus (EC50 10.6 μg/mL). Ampelocissus tomentosa, Boenninghausenia albiflora, Dichrocephala integrifolia, and Kalanchoe pinnata significantly reduced C. elegans motility, comparable to levamisole. Conclusions. In countries like Nepal, with a high burden of infectious and parasitic diseases, and a current health system unable to combat the burden of diseases, evaluation of local plants as a treatment or potential source of drugs can help expand treatment options. Screening plants against a broad range of pathogens (bacteria, viruses, fungi, and parasites) will support bioprospecting in Nepal, which may eventually lead to new drug development.

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An Update on Natural Antileishmanial Treatment Options from Plants, Fungi and Algae

Koko

Nasr

Khan

et al. 2021

Chemistry & Biodiversity

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Efficient drugs for the treatment of leishmaniasis, which is classified as a neglected tropical disease, are sought for. This review covers potential drug candidates from natural plant, fungus and algae sources, which were described over the last six years. The identification of these natural antileishmanials often based on the knowledge of traditional medicines. Crucial insights into the activities of these natural remedies against Leishmania parasites and against infections caused by these parasites in laboratory animals or patients are provided and compared with selected former active examples published more than six years ago. In addition, immuno-modulatory natural antileishmanials and recent developments on combination therapies including natural products and approved antileishmanials are discussed. The described natural products revealed promising data warranting further efforts on the discovery and development of new antileishmanials based on patterns from nature.

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In vitro Antileishmanial and Antimalarial Activity of Selected Plants of Nepal

Cited by 20 publications

References 38 publications

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Evaluation of In vivo Antimalarial Activities of leaves of Moringa oleifera against Plasmodium berghei in Mice

Antibacterial, Antifungal, Antiviral, and Anthelmintic Activities of Medicinal Plants of Nepal Selected Based on Ethnobotanical Evidence

An Update on Natural Antileishmanial Treatment Options from Plants, Fungi and Algae

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