In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava L.

Abstract

Aim:This study was designed to examine the chemical composition and in vitro antimicrobial potential of methanolic extract of Psidium guajava Linn (Myrtaceae).Materials and Methods:The inhibitory effect of methanolic extract of P. guajava was tested against three bacterial and two fungal strains by using the paper disc diffusion method.Results:The methanolic extract exhibited antibacterial activity against E. coli with minimum inhibitory concentration, 0.78 μg/ml, minimum bactericidal concentration of 50 μg/ml, and appreciable antifungal activity with minimum inhibitory concentration of 12.5 μg/ml. Preliminary phytochemical analysis of methanolic extract revealed the presence of antimicrobial compounds such as flavonoids, steroids, and tannins, which may contribute for the antimicrobial action of P. guajava.Conclusion:The extract was found to be bacteriostatic and fungistatic in action.

Phytopharmacological overview of Psidium guajava Linn.

“…It interferes with the envelope subunit gp41 form the critical 6-HB structure, thus inhibit the entry of HIV into target cells. Dhiman et al 37 found that methanolic extract of P. guajava leaf has bacteriostatic and fungistatic in action. P. guajava tea showed efficient control of epidemic and pandemic influenza viruses, including oseltamivir-resistant strains, and its broad target blockage makes it less likely to lead to the emergence of viral resistance.…”

Antibacterial Activity of Eucalyptus Tereticornis and Psidium Guajava on Bacillus Thurangenisis, Bacillus Cereus and Pseudomonas Aeruginosa Isolated From Used Industrial Coolant

“…[27,28] Plants belonging to myrtacea family (Psidium gaujava, and Eucalyptus tereticornis) were belived to Badrunnisa et al…”

“…[30,31,32] Previous report on phytochemical screening of Psidium gaujava [28] , indicate the presence of carbohydrates, flavinoids, glyceroids, steroids and tannins as the main constituents. In contrast the present study reveals the presence of tannins, steroids, flavinoids, terpenoids, cardiac glyceroids, alkaloids and reducing sugars with both ethanol and methanol extracts of Psidium gaujava.…”

Evaluation of the Local Anaesthetic Effect of Crassocephalum Crepidioides in Suitable Experimental Animal Models.

“…Fifty grams of this powder was then extracted with methanol using a soxhlet apparatus. (6) The extract was evaporated to dryness and the final yield was 14% which was stored in the refrigerator and fresh sterile solutions were prepared as and when required by dissolving it in normal saline.…”