In vitro antimicrobial and antimycobacterial activity and HPLC–DAD screening of phenolics from Chenopodium ambrosioides L.

Abstract

The main objective of this study was to demonstrate the antimicrobial potential of the crude extract and fractions of Chenopodium ambrosioides L., popularly known as Santa-Maria herb, against microorganisms of clinical interest by the microdilution technique, and also to show the chromatographic profile of the phenolic compounds in the species. The Phytochemical screening revealed the presence of cardiotonic, anthraquinone, alkaloids, tannins and flavonoids. The analysis by HPLC–DAD revealed the presence of rutin in the crude extract (12.5 ± 0.20 mg/g), ethyl acetate (16.5 ± 0.37 mg/g) and n-butanol (8.85 ± 0.11 mg/g), whereas quercetin and chrysin were quantified in chloroform fraction (1.95 ± 0.04 and 1.04 ± 0.01 mg/g), respectively. The most promising results were obtained with the ethyl acetate fraction, which inhibited a greater number of microorganisms and presented the lowest values of MIC against Staphylococcus aureus and Enterococcus faecalis (MIC = 0.42 mg/mL), Pseudomonas aeruginosa (MIC = 34.37 mg/mL), Paenibacillus apiarus (MIC = 4.29 mg/mL) and Paenibacillus thiaminolyticus (MIC = 4.29 mg/mL). Considering mycobacterial inhibition, the best results were obtained by chloroform fraction against M. tuberculosis, M. smegmatis, and M. avium (MIC ranging from 156.25 to 625 μg/mL). This study proves, in part, that the popular use of C. ambrosioides L. can be an effective and sustainable alternative for the prevention and treatment of diseases caused by various infectious agents.

The Antibacterial Activity Screening of the Extracts of Some Moroccan Medicinal Plants

“…Finally, the leaf extracts of Dysphania ambrosioide did not show any antibacterial activity against targets bacterial studied, as it was already demonstrated for Dysphania ambrosioide collected from Meknés city (Morocco) with no antibacterial effect [79] in contrary to Brazilian [80] or Pakisani [81] Dysphania ambrosioide which showed good antibacterial activity. These difference can be related to the geographical localization and environmental factors connected, who are one of the major determinants of plant secondary metabolites [82,83] responsible of the plant antimicrobial activity.…”

In Vitro Bioactivity of Methanolic Extracts from Amphipterygium adstringens (Schltdl.) Schiede ex Standl., Chenopodium ambrosioides L., Cirsium mexicanum DC., Eryngium carlinae F. Delaroche, and Pithecellobium dulce (Roxb.) Benth. Used in Traditional Medicine in Mexico

“…Sousa et al (2012) could not detect antimicrobial activity from an ethanolic extract of the aerial parts up to 500 μ g/ml [ 55 ], as we did not observe it either for a methanolic extract from the leaves. Jesus et al (2017) determined for an ethanolic crude extract from leaves only very high MICs against Mycobacterium tuberculosis (1.25 mg/ml), E. faecalis (4.29 mg/ml), and P. aeruginosa (68.75 mg/ml) [ 56 ]. Generally, the whole aerial part is not considered (very) cytotoxic: Ruffa et al (2002) did not detect cytotoxic effects up to 1,000 μ g/ml for HepG2 [ 57 ], Koba et al (2009) found a CC 50 = 700 μ g/ml for HaCaT [ 50 ], and Barros et al (2013) determined CC 50 of 264 to 319 μ g/ml for HeLa, HepG2, and HCT-15 [ 58 ]; those values are in the range of CC 50 = 563 ± 66 μ g/ml ELM that we report for Caco-2.…”

Potencial fitoterápico do Chenopodium Ambrosioides L. na Odontologia

“…Há relatos de seu uso na medicina popular para o tratamento de feridas, leucorreia, processos inflamatórios, como agente antihelmíntico e como anti-séptico oral . Estudos anteriores demonstraram seu potencial medicinal contra a ação de endoparasitas e fungos (Jesus et al, 2018), dentre estes, Development, v. 9, n. 7, e818974983, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i7.4983 algumas espécies de Candida. Além disso, atividades ansiolíticas, curativas de feridas cutâneas, anti-inflamatórias (Trivellato Grassi et al, 2013;Penha et al, 2017) e atividades antioxidantes foram descritas.…”