In-vitro antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinone isolated from Aegle marmelos (Linn.) Correa

Abstract

BackgroundPlant metabolites have wide applications and have the potential to cure different diseases caused by microorganisms. The aim of the study was to evaluate the antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinine isolated from Aegle marmelos (Linn.) Correa.MethodsA compound was obtained by eluting the crude extract, using varying concentrations of the solvents by the chromatographic purification. Broth micro dilution method was used to assess the antimicrobial activity and anticancer study was evaluated using MTT assay. Larvicidal activity was studied using leaf disc no-choice method.ResultsBased on the IR, 13C NMR and 1H NMR spectral data, the compounds were identified as quinone related antibiotic. It exhibited significant activity against Gram positive and Gram negative bacteria. The lowest Minimum Inhibitory Concentration (MIC) of the compound against Bacillus subtilis and Staphylococcus aureus was 100 and 75 μg mL−1 respectively. Against Escherichia coli and Pseudomonas aeruginosa it exhibited MIC value of 25 μg mL−1. The MIC of the compound against Aspergillus niger, A. clavatus, Penicillium roqueforti was 20 μg mL−1 and that against Fusarium oxysporum (20 μg mL−1), A. oryzae (40 μg mL−1), and Candida albicans (60 μg mL−1), respectively. It showed effective antibiofilm activity against E. coli, S. typhii and P. aeroginosa at 8 μg mL−1 and did not exhibit considerable cytotoxic activity against Vero and HEP2 cell lines. Additionally, the compound documented significant antifeedant and larvicidal activities against Helicoverpa armigera and Spodoptera litura at 125, 250, 500 and 1000 ppm concentrations.ConclusionThe results concluded that the compound can be evaluated further in industrial applications and also an agent to prepare botanical new pesticide formulations.

In VitroAntibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal PlantPittosporum tetraspermum

“…The crude ethyl acetate extract was screened for antimicrobial activity against Gram positive and Gram negative bacteria by disc diffusion method [ 10 ]. The active ethyl acetate extract was chromatographed over silica gel (Acme's 100–200 mesh).…”

“…The minimum inhibitory concentration of the compound was performed according to the reference method [ 10 ]. The compound was dissolved in water together with 2% dimethyl sulfoxide (DMSO).…”

Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa

“…Streptomycin (30 lg/ml) was used as control to compare the effectiveness of AgNPs and plant extracts against tested bacteria. The minimum inhibitory concentration (MIC) was studied using twofold dilution method with initial test concentration of 30 lg/ml by following Rejiniemon et al 2014. The MIC was calculated as the lowest concentration of the AgNPs inhibiting the visual growth of the test cultures on the agar plates.…”

<i>In Vitro</i> Antibacterial, Antifungal and Other Medical Properties of Endangered Medicinal Plant Seeds

“…Standard reported method was followed for the determination of minimum inhibitory concentration in 96 well plate [6].…”

“…The phytochemicals such as phenolic compounds, flavanoids, anthocyanins, alkaloids, terpernoids, saponins and quinine molecules obtained from traditional medicinal plants are mainly responsible for their potential activity [6] [7] [8] [9].…”