In vitro antimicrobial susceptibility ofListeria monocytogenes isolated in the UK and otherListeria species

MacGowan, A. P.; Holt, H. A.; Bywater, M. J.; Reeves, D. S.

doi:10.1007/bf02184692

Cited by 33 publications

(34 citation statements)

References 6 publications

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“…The isolates showed higher susceptibility to cefuroxime, cefoxitin, ceftriaxone, and cephalotin. Strains of 100 100 100 100 100 100 0 100 100 0 100 100 100 100 100 100 100 100 C. glucuronolyticum 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 different species showed resistance to ceftazidime and dissociated susceptibility to cephalotin and cefuroxime as observed in previous studies with Listeria monocytogenes (14). Only C. glutamicum and C. glucuronolyticum strains were susceptible to the β-lactams.…”

Section: Resultssupporting

confidence: 47%

Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil

Camello

Mattos‐Guaraldi

Formiga

et al. 2003

Braz. J. Microbiol.

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Over a five-year period, 163 strains of Corynebacterium sp. were recovered from different clinical specimens of patients from a Brazilian University hospital. Genitourinary tract and intravenous sites specimens were the most frequent sources of corynebacteria (46.62%). Corynebacterium amycolatum (29.55%), Corynebacterium minutissimum (20.45%) and Corynebacterium pseudodiphtheriticum (13.63%) were the predominant species found in genitourinary tract. C. minutissimum (24.14%) and Corynebacterium propinquum (17.24%) in surgical and/or other skin wounds and abscesses; Corynebacterium xerosis (25%), C. amycolatum (21.87%) and C. pseudodiphtheriticum (18.75%) in intravenous sites; C. pseudodiphtheriticum (33.33%) and C. propinquum (33.33%) in lower respiratory tract. Microorganisms were all susceptible to vancomycin and most of the species was predominantly resistant to β-lactams. Antimicrobial susceptibility patterns of corynebacteria were not predictable. Multiple antibiotic resistance observed in C. jeikeium was also found among C. xerosis, C. minutissimum, C. afermentans, C. propinquum, C. amycolatum and C. pseudodiphtheriticum strains. Data suggest awareness of clinicians and microbiologists to nosocomial infections especially due to antimicrobial multiresistant strains of Corynebacterium sp.

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Section: Resultssupporting

confidence: 47%

Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil

Camello

Mattos‐Guaraldi

Formiga

et al. 2003

Braz. J. Microbiol.

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“…These organisms had been considered susceptible to penicillins, but our results and other scattered reports (10,13,17,18,20,27,28,31) suggest that penicillin-resistant strains are appearing. The dissociated susceptibility of L. monocytogenes to cephalothin and cefuroxime has been described previously, and this organism is also resistant to ceftazidime (11). Our results show an increase in the MICs of ampicillin for L. monocytogenes in comparison with those given in other reports (21).…”

Section: Discussioncontrasting

confidence: 45%

Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents

Soriano

Zapardiel

Nieto

1995

Antimicrob Agents Chemother

168

117

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The susceptibilities of 265 strains of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents were tested. Most strains were susceptible to vancomycin, doxycycline, and fusidic acid. Corynebacterium jeikeium and Corynebacterium urealyticum were the most resistant organisms tested. Resistance to ␤-lactams, clindamycin, erythromycin, azythromycin, ciprofloxacin and gentamicin was common among strains of Corynebacterium xerosis and Corynebacterium minutissimum. Ampicillin resistance among Listeria monocytogenes was more prevalent than previously reported. Optochin, fosfomycin, and nitrofurantoin showed very little activity against most organisms tested, but the use of nitrofurantoin as a selective agent in culture medium may prevent the recovery of some isolates. Except for the unvarying activity of vancomycin against Corynebacterium species, the antimicrobial susceptibilities of the latter to other antibiotics are usually unpredictable, such that susceptibility tests are necessary for selecting the best antimicrobial treatment.During the last two decades a renewed interest in Corynebacterium species and other non-spore-forming gram-positive bacilli has emerged among clinicians and microbiologists alike (2,8,9,32). Infections caused by these organisms are emerging, new species are being recognized (2, 9), and infections by toxigenic and nontoxigenic Corynebacterium diphtheriae strains are also being described with increasing frequency, indeed, in countries where diphtheria had been totally or almost eradicated (7, 26). However, this renewed interest has not been followed by an in-depth study to determine the antimicrobial susceptibilities of such organisms. Most available data come from scattered case reports, studies on a particular organism, or very old reports, sometimes published before 1960 (9).The aim of the study described here was to determine the antimicrobial susceptibilities of a variety of organisms, most of which were isolated from clinical specimens, against 18 antimicrobial agents. MATERIALS AND METHODSBacterial strains. We tested 265 strains of Corynebacterium species and other non-spore-forming gram-positive bacilli obtained from the following different sources: clinical samples (n ϭ 141), skin (n ϭ 25), bacterial collections (n ϭ 19), and referrals to our laboratory from other institutions (n ϭ 80). Strains from clinical samples were isolated during the period from 1985 to 1993. All strains were identified by conventional methods (2) and also by using the API Coryne system (25), were stored frozen in 10% skim milk, and were maintained at Ϫ70ЊC until use.Antimicrobial agents. The following antibiotics were kindly provided by the manufacturers as powders for in vitro study: ampicillin and oxacillin (SmithKline Beecham Laboratories, Worthing, United Kingdom), cephalothin and vancomycin (Lilly, S.A., Madrid, Spain), cefuroxime (Glaxo Laboratories, Madrid, Spain), imipenem (Merck Sharp & Dohme, Madrid, Spain), tetracycline, doxycycline, and azithromycin (Pf...

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“…We have studied the genetic basis of tetracycline resistance in 25 strains of L. monocytogenes isolated in the United Kingdom from human listeriosis between 1967 and 1990 (18,19). Among these strains, 24 were Tcr Mcr and contained sequences related to tet(M).…”

Section: Discussionmentioning

confidence: 99%

“…Prognosis of listeriosis remains serious, and even with appropriate antibiotic therapy, listeriosis is fatal in approximately one-third of all cases (10). Most strains of L. monocytogenes are uniformly susceptible to antibiotics (18,19). Acquired resistance to chloramphenicol, erythromycin, streptomycin, and tetracycline in two clinical isolates of L. monocytogenes has been reported (27,30).…”

mentioning

confidence: 99%

Genetic basis of tetracycline resistance in clinical isolates of Listeria monocytogenes

Poyart-Salmeron

Trieu-Cuot

Carlier

et al. 1992

Antimicrob Agents Chemother

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The genetic basis of tetracycline resistance was studied in 25 clinical isolates of Listeria monocytogenes. Resistance to tetracycline was associated with resistance to minocycline and due to the presence of the tet(M) gene in 24 strains. Association of tet(M) with int-Tn, the gene encoding the protein required for the movements of Tn1545-like conjugative transposons, was found in all strains. Cotransfer of tet(M) and int-Tn among L. monocytogenes cells and from L. monocytogenes to Enterococcusfaecalis was detected in 7 of the 12 strains studied at frequencies similar to those obtained with the prototype element Tnl545. tet(L), the secondmost prevalent tetracycline resistance gene in enterococci and streptococci, was detected in the remaining strain, where it was borne by a 5-kb plasmid. These observations indicate that two types of movable genetic elements, transposons and plasmids, in enterococci and streptococci are responsible for emergence of drug resistance in L. monocytogenes.

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In vitro antimicrobial susceptibility ofListeria monocytogenes isolated in the UK and otherListeria species

Cited by 33 publications

References 6 publications

Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil

Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil

Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents

Genetic basis of tetracycline resistance in clinical isolates of Listeria monocytogenes

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