In vitro Antioxidant Activity and Phytochemical Screening of Endophytic Extracts of Crotalaria pallida

Govindappa, M.; Bharath, N; Hb, Shruthi; Santoyo, Gustavo

doi:10.5530/ax.2011.3.11

Cited by 21 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…from Salvadora oleoides [344] and Justicia beddomei [345], Aspergillus sp., Bulgaria sp. and Sirococcus conigenus from Potentilla fulgens [346], A. niger and F. oxysporum from Crotalaria pallida [347] [352], Aspergillus awamori and again C. gleosporioides from Rauwolfia serpentina [353].…”

Section: Saponinesmentioning

confidence: 99%

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

2015

View full text Add to dashboard Cite

Abstract:It is known that plant-based ethnomedicine represented the foundation of modern pharmacology and that many pharmaceuticals are derived from compounds occurring in plant extracts. This track still stimulates a worldwide investigational activity aimed at identifying novel bioactive products of plant origin. However, the discovery that endophytic fungi are able to produce many plant-derived drugs has disclosed new horizons for their availability and production on a large scale by the pharmaceutical industry. In fact, following the path traced by the blockbuster drug taxol, an increasing number of valuable compounds originally characterized as secondary metabolites of plant species belonging to the Spermatophyta have been reported as fermentation products of endophytic fungal strains. Aspects concerning sources and bioactive properties of these compounds are reviewed in this paper.

show abstract

Section: Saponinesmentioning

confidence: 99%

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

2015

View full text Add to dashboard Cite

show abstract

“…The phenolic content was found to be 9.16±0.71 mg/g and 12.13±3.96 mg/g of dry weight of extract in CPIMR-2 and CPIL-1, respectively ( Table 2). The presence of similar quantities of phenolic content was also reported in methanolic extract of Aspergillus species and Penicillium species isolated from Tabebuia argentea (19.20 and 16.23 mg/GAE/g) [37].…”

Section: Total Flavonoids and Phenolicsmentioning

confidence: 73%

“…Various fungal species have been also reported to produce these compounds and found to possess the antioxidant potential as well [39]. The production of these secondary metabolites (i.e., phenolics and flavonoids) has been reported in different endophytic fungi, including Aspergillus nidulans and Aspergillus oryzae isolated from G. biloba [40] and Aspergillus niger and Fusarium oxysporum isolated from Crotalaria pallida [37].…”

Section: Total Flavonoids and Phenolicsmentioning

confidence: 99%

Isolation and Characterization of Endophytic Fungi From Calotropis Procera for Their Antioxidant Activity

Nagda

Gajbhiye

Chhatwani³

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: To investigate the antioxidant activity of methanolic extract of endophytic fungi isolated from Calotropis procera plant. Methods:The endophytic fungi were isolated from explants of leaf, stem, and flower of C. procera on potato dextrose broth (PDB) medium. The fungal isolates were mass cultured in PDB. The methanolic extracts of these fungi were prepared and evaluated for the antioxidant activity by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and reducing power assay. The extracts were characterized for the presence of phenolics and flavonoids.Results: A total of 8 endophytic fungi were isolated from C. procera explants. Out of these 8 fungal isolates, the crude extracts of two fungi, i.e. CPIMR-2 and CPIL-1 showed positive antioxidant activity. From the morphological characteristics, the isolates CPIMR-2 and CPIL-1 were identified as Penicillium spp. and Aspergillus spp., respectively. Both the isolates were found to possess antioxidant potential with % inhibition value of 81.16% and 80.97% in the DPPH radical scavenging assay. The phytochemical screening of the methanolic extracts showed the presence of phenolics and flavonoids. The total flavonoids content in Penicillium species and Aspergillus species were found to be 130.50 µg/mg and 94.91 µg/mg of extracts, respectively. The total phenolic content was found to be 9.16 µg/mg and 12.13 µg/mg of extracts in Penicillium species and Aspergillus species, respectively. Conclusions:Endophtyic fungi were found to be present in medicinal value plant C. procera. The probable bioactive component for antioxidant activity possessed by the fungi would be the flavonoids and phenolics. These metabolite produced by endophytic fungi from C. procera need to be explored further as potential source of novel natural antioxidant compound.

show abstract

“…On the other hand, the methanol extract of T. stans leaf was reported to possess significant wound healing property (Das et al, 2010). Additionally, the ethanol, methanol and water extracts of T. stans have been reported for good antimicrobial effects on some human pathogenic bacteria and antioxidant activity (Govindappa et al, 2011;Senthilkumar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies found that Tecoma genus possess various bioactive compounds that are reported to exhibit various pharmacological activities such as antioxidant, antimicrobial and antifungal activities (Karou et al, 2006;Raju et al, 2011;Govindappa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. ex Kunth against nine species of pathogenic bacteria

Salem¹,

Gohar²,

Camacho³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Tecoma stans of the Bignoniaceae family, is an important medicinal plant. Different extracts of the leaves and branches of T. stans were evaluated as antibacterial potential against the growth of some human bacterial strains using the disc diffusion and minimum inhibitory concentration (MIC) methods as well as the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and total flavonoid contents were determined. The different extracts of T. stans were obtained by successive solvent extraction with methanol and its fractions; ethyl acetate (EtOAc), chloroform (CHCl 3 ), n-butanol and aqueous. The methanol, EtOAc and CHCl 3 extracts of T. stans showed significant effect against the tested bacteria (Bacillus subtilis, Micrococcus luteus, Sarcina lutea, Staphylococcus aureus, Escherichia coli, Serratia marcescens, Salmonella typhi, Proteus vulgaris and Pseudomonas aeruginosa). The methanol extract of the leaves exhibited the highest amount of total phenolics (50.3±3 mg gallic acid equivalents/g extract) and flavonoids (40.66±5.03 mg catechin equivalents/g extract). The EtOAc fraction of leaves and branches extracts showed the highest total antioxidant activity (%) with 83.4±0.31 and 82.06±0.54%, respectively followed by CHCl 3 fraction of leaves extracts (79.17±0.20%). These findings provide scientific evidence to support traditional medicinal uses of T. stans and indicate a promising potential for the development of an antibacterial and antioxidant agent from T. stans trees.

show abstract

In vitro Antioxidant Activity and Phytochemical Screening of Endophytic Extracts of Crotalaria pallida

Cited by 21 publications

References 28 publications

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

Isolation and Characterization of Endophytic Fungi From Calotropis Procera for Their Antioxidant Activity

Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. ex Kunth against nine species of pathogenic bacteria

Contact Info

Product

Resources

About