In vitro Antioxidant Activity of Polygonium hyrcanicum, Centaurea depressa, Sambucus ebulus, Mentha spicata and Phytolacca americana

Hosseinimehr, Seyed Jalal; Pourmorad, F.; Shahabimajd, N; Shahrbandy, Kami; Hosseinzadeh, R

doi:10.3923/pjbs.2007.637.640

Cited by 42 publications

(21 citation statements)

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“…The effect of HRAM sim on nitric oxide was expected, given the previously demonstrated antioxidant properties of HRAM [13] and Mentha spicata [25], as well as reported inhibitory effects of Mentha sp . on nitric oxide formation [26,27].…”

Section: Discussionmentioning

confidence: 76%

Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

Pearson

Fletcher

Kott

et al. 2010

BMC Complement Altern Med

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BackgroundA variety of mint [Mentha spicata] has been bred which over-expresses Rosmarinic acid (RA) by approximately 20-fold. RA has demonstrated significant anti-inflammatory activity in vitro and in small rodents; thus it was hypothesized that this plant would demonstrate significant anti-inflammatory activity in vitro. The objectives of this study were: a) to develop an in vitro extraction procedure which mimics digestion and hepatic metabolism, b) to compare anti-inflammatory properties of High-Rosmarinic-Acid Mentha spicata (HRAM) with wild-type control M. spicata (CM), and c) to quantify the relative contributions of RA and three of its hepatic metabolites [ferulic acid (FA), caffeic acid (CA), coumaric acid (CO)] to anti-inflammatory activity of HRAM.MethodsHRAM and CM were incubated in simulated gastric and intestinal fluid, liver microsomes (from male rat) and NADPH. Concentrations of RA, CA, CO, and FA in simulated digest of HRAM (HRAMsim) and CM (CMsim) were determined (HPLC) and compared with concentrations in aqueous extracts of HRAM and CM. Cartilage explants (porcine) were cultured with LPS (0 or 3 μg/mL) and test article [HRAMsim (0, 8, 40, 80, 240, or 400 μg/mL), or CMsim (0, 1, 5 or 10 mg/mL), or RA (0.640 μg/mL), or CA (0.384 μg/mL), or CO (0.057 μg/mL) or FA (0.038 μg/mL)] for 96 h. Media samples were analyzed for prostaglandin E2 (PGE2), interleukin 1β (IL-1), glycosaminoglycan (GAG), nitric oxide (NO) and cell viability (differential live-dead cell staining).ResultsRA concentration of HRAMsim and CMsim was 49.3 and 0.4 μg/mL, respectively. CA, FA and CO were identified in HRAMsim but not in aqueous extract of HRAM. HRAMsim (≥ 8 μg/mL) inhibited LPS-induced PGE2 and NO; HRAMsim (≥ 80 μg/mL) inhibited LPS-induced GAG release. RA inhibited LPS-induced GAG release. No anti-inflammatory or chondroprotective effects of RA metabolites on cartilage explants were identified.ConclusionsOur biological extraction procedure produces a substance which is similar in composition to post-hepatic products. HRAMsim is an effective inhibitor of LPS-induced inflammation in cartilage explants, and effects are primarily independent of RA. Further research is needed to identify bioactive phytochemical(s) in HRAMsim.

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Section: Discussionmentioning

confidence: 76%

Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

Pearson

Fletcher

Kott

et al. 2010

BMC Complement Altern Med

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“…DPPH stable free radical scavenging method can be used to evaluate the antioxidant activity of a extracts in a short time (Tiwari, 2001;Hosseinimehr et al, 2007). The values of the 50% inhibition concentration (IC50) of leaf, fruit, stem and root extracts of P. americana for the DPPH radical were 10991.76 mg L , respectively (Table 3).…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.

Boo¹,

Park²,

Woo³

et al. 2015

Korean J. Crop Sci.

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This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphylococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.

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“…ROS such as superoxide anion, peroxide, hydroperoxide, hydroxyl radical and hydroxyl ion are generated during metabolic reactions in the body as they cause deterioration to lipid cells, essential proteins, DNA and immune cells (Manain et al, 2008;Mbaebie et al, 2012). They are also known to cause oxidative stress leading to the pathogenesis of several diseases in humans such as cancer, diabetes, cardiovascular diseases, atherosclerosis and chronic inflammation (Hosseinimehr et al, 2007;Raghuveer et al, 2009). Free radicals can be eliminated naturally or by synthetic antioxidant agents.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical constituents and antioxidant properties of acetone extract of <i>Cleome gynandra</i> (L.) growing in the Eastern Cape, South Africa.

Sowunmi¹,

Afolayan²

2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Several wild vegetables have been reported for their therapeutic benefits in South Africa. Many of these plants including Cleome gynandra (L.) lack scientific reports on its significance in folkloric medicine. Therefore, this study was undertaken to evaluate quantitatively the compositions of phytochemicals and antioxidant properties of acetone extract of different parts of C. gynandra. Materials and Method: Antioxidant activities were assessed against ferric reducing power, ABTS (2, 2'-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and NO (nitric oxide) radical scavenging activities. Total phenolics, flavonoids, flavanols, proanthocyanidins, tannins, saponins and alkaloids were also investigated. Results: Amongst the different plant parts, the leaf extract had the highest concentration of total phenolics (126.79 ± 0.55 mg/g), flavonoids (40.58 ± 0.06 mg/g) and flavanols (42.41 ± 0.05 mg/g) while the stem extract had the highest amount of proanthocyanidins (419.01 ± 0.67 mg/g) compared to the leaves (403.29 ± 0.89 mg/g) and fruits (107.18 ± 0.59 mg/g). The reducing power of the extracts was significantly higher than that of the standard drugs used in a concentration dependent manner. The activities of the plant extracts against ABTS, DPPH and NO radicals were dose responsive with IC 50 value of 0.2, 0.1 and 0.03 mg/g respectively. Conclusion: C. gynandra possesses high secondary metabolites which accounts for its strong antioxidant ability thus justifying its use as natural occurring antioxidants in folkloric medicine. The study encourages a regular consumption of this wild vegetable in order to avert oxidative stress related diseases.

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In vitro Antioxidant Activity of Polygonium hyrcanicum, Centaurea depressa, Sambucus ebulus, Mentha spicata and Phytolacca americana

Cited by 42 publications

References 6 publications

Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.

Phytochemical constituents and antioxidant properties of acetone extract of <i>Cleome gynandra</i> (L.) growing in the Eastern Cape, South Africa.

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