In Vitro Antioxidant Activity, Total Phenolic Contents and Phytochemical Evaluation From Crude Extracts of an Endangered Plant Cordia Macleodii Hook. F. Thomson

Chandrakar, Jagriti; Dubey, Satish; Ojha, Kundan; Dehariya, Rashmi; Dixit, Ashwini Kumar

doi:10.32553/jbpr.v8i3.620

Cited by 3 publications

(3 citation statements)

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“…Comparable correlation was found between the total phenolic content and antioxidant activities from six medicinal plants and C. fistula during the study of antioxidants potential of their different parts (29,30). Likewise flavonoids such as kaempferol, rutin, myricetin and quercetin also contributed as free radical scavenger (31)(32)(33). The data obtained from the present study revealed that, all selected folk medicinal plants significantly (p<0.05) followed the following trends, i.e.…”

Section: Discussionsupporting

confidence: 42%

Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

et al. 2020

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Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 μg GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (μg QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p<0.05). The lowest IC50 (μg/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.

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Section: Discussionsupporting

confidence: 42%

Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

et al. 2020

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“…Tests conducted in laboratories on a few Cordia species have revealed their antibacterial potential. This antibacterial activity, which has been shown in vitro, suggests that Cordia macleodii may possess similar properties (Cordia and Hook, 2019). These qualities are promising for the treatment of infections.…”

Section: Cordia Macleodii and Related Speciesmentioning

confidence: 69%

“…Where, C = Total content of phenolic compounds (GAE mg gm-1 of plant extract), c = concentration of gallic acid established from the calibration curve (mg ml-1), V = The volume of extract in ml and m = The weight of crude plant extract in gm (Cordia and Hook, 2019).…”

Section: = (C × V) / Mmentioning

confidence: 99%

Evaluation of Composition and Antioxidant Potential of Cordia macleodii

Adile,

Seth

2024

JBPR

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Cordia macleodii is one of the widely used traditional medicinal herbs. The current work was undertaken to systematically extract bioactive components from the fresh plant parts and evaluate their antioxidant potential. Using ethanol as the solvent, volatile chemical components were extracted from the plant material by Soxhlet extraction. Subsequently, GC-HR-MS and FTIR analysis were used to further evaluate the extract. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2,2'- Azinobis [3-ethylbenzothiazoline-6-sulfonic acid (ABTS)] were used to evaluate the antioxidant potentialof the extract in vitro. Quantification of total flavonoid content (TFC) and total phenolic content (TPC) was done against standard solutions of quercetin and gallic acid, which were used in the study as positive controls. The GC-HR-MS analysis reveals two bioactive compounds [(1H)- pyrrol-3-propanoic acid,2 ethoxy carbonyl-4-ethoxy carbonyl methyl]-5,5’-methylene, bis-diethyl ester and Spino [9,9’] difluorene,2,2’-[2,5,8,11-tetraoxado decane-1,12-diye). The FTIR spectrum showed that there were several different functional groups present. Findings of in vitroantioxidant tests revealed that the leaves had the highest total phenolic content (402.19 ± 0.025 mg GAE/g), the highest total phenolic content (93.59 ± 0.01), and the highest ABTS (102.59 ± 0.001) of any plant. To fully evaluate this plant's therapeutic potential, more pharmacognostic research is required. Keywords: GC-HR-MS, FTIR, TPC, TFC, Antioxidant

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Antioxidant Phenolics of Justicia adhatoda L. and Cordia dichotoma Frost. Promote Thrombolytic Activity through Binding to a Serine Protease, Tissue Plasminogen Activator Protein

Paul,

Nag

2023

Biomedical and Biotechnology Research Journal (BBRJ)

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Background: The tissue plasminogen activator (tPA) protein dissolutes fibrin clots and prevents the disease like thrombosis. The current study aimed to study the tPA-promoting activity of bioactive molecules of Justicia adhatoda L (JA) and Cordia dichotoma Frost (CD). Methods: The phytochemical characterization of methanolic and aqueous extracts of JA and CD stems was performed through qualitative analysis, Fourier-transform infrared spectroscopy (FTIR), and biochemical tests (total phenolic and total flavonoid content [TPC and TFC]). The bioactivity of the extracts was studied through total antioxidant capacity (TAC) and ferric-reducing antioxidant potential (FRAP) assays. Finally, forty phytocompounds from JA and CD were identified from the literature, and in silico molecular docking study was performed to target tPA protein (PDB id 1A5H, Chain A, X-ray diffraction, resolution 2.90 Å). Results: Various phytochemical classes were identified from extracts, through qualitative and FTIR analysis. TPC and TFC were estimated from the JA and CD extracts within the range of 9.34–28.67 mg gallic acid equivalent/100 g of extract weight (EW) and 2.48–16.17 mg quercetin equivalent/100 g of EW, respectively. The aqueous extract of CD showed the highest TAC of 14.90 ascorbic acid equivalent (AAE)/100 g of EW, and the methanolic extract of JA had the highest FRAP activity of 27.77 mg AAE/100 g EW. The molecular docking study showed that apigenin 6,8-di-glucopyranoside had the highest binding potential toward the tPA (−9.380 kcal/mol). Conclusion: It can be concluded that antioxidant phytochemicals of JA and CD could promote the tPA activity, thereby promoting thrombolytic activity.

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In Vitro Antioxidant Activity, Total Phenolic Contents and Phytochemical Evaluation From Crude Extracts of an Endangered Plant Cordia Macleodii Hook. F. Thomson

Cited by 3 publications

References 15 publications

Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

Evaluation of Composition and Antioxidant Potential of Cordia macleodii

Antioxidant Phenolics of Justicia adhatoda L. and Cordia dichotoma Frost. Promote Thrombolytic Activity through Binding to a Serine Protease, Tissue Plasminogen Activator Protein

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