In vitro antioxidant and anti-diabetic analysis of Andrographis echioides and Andrographis paniculata ethanol extract

Abstract

It is of interest to analyse and compare the antioxidant and anti-diabetic activity of ethanolic extracts of Andrographis echioides and Andrographis paniculata. Andrographis echioides and Andrographis paniculata were collected from a local farm. In vitro antioxidant activity was assessed by the potential of Piperine, Lupeol, beta sitosterol; DPPH free radical scavenging assay was performed by Liyana Pathirana and Shahidi method. In vitro anti-diabetic activity was assessed by alpha amylase inhibitory activity and alpha glucosidase inhibitory activity. The data were analysed by one-way-ANOVA to check the statistical significance among the groups and considered at the levels of p<0.05. Both the ethanolic extracts of Andrographis echioides and Andrographis paniculata showed significant antioxidant and anti-diabetic potential in a dose-dependent manner (100-500μg) and can be used as potential antidiabetic agents. Similar to antioxidant potential, Andrographis paniculata exhibited an increased anti-diabetic potential compared to Andrographis echioides. Data shows that the ethanolic extracts of Andrographis echioides and Andrographis paniculata possessed antioxidant and anti-diabetic activity and hence our present findings conclude that both plants can be considered for the development of natural drugs for the management of diabetes.

Comparison of diterpenoid contents and dissolution profiles of selected Andrographis paniculata crude and extract capsules

“…AP extract and its active diterpenoids have been extensively studied for their pharmacological activities such as antiviral, anti-inflammatory, antioxidant, anticancer, hepatoprotective, immunostimulatory, antihyperglycemic, and neuroprotective effects. [3][4][5][6][7][8][9] In 2019, the outbreak of the coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China. The treatment strategies for critical COVID-19 patients are lacking, with the only use of available antiviral medicines and urgently approved COVID-19 vaccines.…”

“…The active diterpenoids in AP were andrographolide (AP1), 14‐deoxy‐11,12‐didehydroandrographolide (AP3), neoandrographolide (AP4), and 14‐deoxyandrographolide (AP6) (Figure 1). AP extract and its active diterpenoids have been extensively studied for their pharmacological activities such as antiviral, anti‐inflammatory, antioxidant, anticancer, hepatoprotective, immunostimulatory, antihyperglycemic, and neuroprotective effects 3–9 …”