In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

Abstract

The aim of the present study was to examine the antioxidant and anti-inflammatory activities of three Moroccan ethno medicinally important plants. The antioxidant activity was performed using β-Carotene bleaching assay, DPPH, ABTS and Ferric reducing assays. In vitro anti-inflammatory assays was also studied through the evaluation of membrane stabilization potential and inhibition of protein denaturation. Results revealed that Lawsonia inermis L leaves extract had significantly higher phenolics-content (5.23 g GAE/100g DW) and exhibited the highest antioxidant activity based on DPPH (IC50 = 18.26 µg/mL). The highest flavonoids content was found in Rosa damascena. L flower (3.97 RE/100g DW) which shows the highest antioxidant activity based on ABTS (30.39 mmol TE/100g DW), FRAP (38.54 mmol TE/100gDW) and β-Carotene bleaching assay (IC50 = 56.33 µg/mL). Concerning the anti-inflammatory potency, the highest membrane stabilization effect was found in Rosa damascena. L flower (IC50 = 125.02 µg/mL) and the highest anti-denaturation of protein effect was found in Lawsonia inermis L leaves extract (IC50 = 103.21 µg/mL). Consequently, to conclude flavonoids and related phenolics present in studied plant materials may possibly be responsible for the important antiinflammatory and antioxidant activities.

In Vitro Anti Inflammatory and Anti Microbial Properties of Allophylus Serratus Leaf Extracts.

“…HRBC stabilization evaluation of the leaf extracts was carried out according to the Gandhisan et al, (1991), Murugan and Parimelazhagan, (2014) and Bouhlali et al (2016) methods with some modifications. Whole human blood was collected from volunteers (who had not taken any NSAIDS for 2 weeks prior to the experiment) and mixed with equal volume of Alsever's solution which was prepared by dissolving 2% dextrose, 0.80% sodium citrate, 0.05% citric acid and 0.42% sodium chloride in sterilized double distilled water.…”

“…There reason why these assay methods selected was due to ethical challenges and the nonexistence of rationale to use animals for pharmacological research of new chemical compounds (Bouhlali et al 2016). …”

“…The possible reason for the anti inflammatory activities of the plant is the presence of Phenolics, flavonoids, tannins and saponins which have anti inflammatory activity (Oyedapo, 2001). The in vitro anti-inflammatory effect of plant material may be due to synergistic effect rather than single constituent of their polyphenols contents (Bouhlali et al 2016). This study also showed that the type of solvent used in extraction is important to find polyphenolic compounds from plant extracts.…”

Bioactive Compounds and Antioxidant, Antiperoxidative, and Antihemolytic Properties Investigation of Three Apiaceae Species Grown in the Southeast of Morocco

“…Quantification of phenols within the three extracts was executed using Folin–Ciocalteu's reagent, following the protocol reported by Bouhlali et al [ 17 ] whose principle relies on the decay of phosphomolybdic-phosphor tungstic acid caused by phenol compounds in an alkaline medium [ 18 ]. To proceed, 500 µ L of Folin–Ciocalteu's reagent diluted ten-fold in water was poured onto 100 µ L of a suitable diluted hydromethanolic plant extract and, then, 400 µ L of sodium carbonate solution (7.5% w/v).…”

Antioxidant, anti-inflammatory and anticoagulant activities of three Thymus species grown in southeastern Morocco

“…Moreover, Oteiza et al [28] have suggested that interactions of polyphenols at the surface of bilayers through hydrogen bonding can act to reduce the access of deleterious molecules (i.e., oxidants), therefore protecting the structure and function of membranes. Several reports have mentioned that plant flavonoids and polyphenols have anti-inflammatory characteristics [29].…”