In vitro antioxidant and anti-lipoperoxidative activities of bark extracts of Xylopia aethiopica against ion-mediated toxicity on liver homogenates

Abstract

Reactive oxygen species (ROS), products of normal cell metabolism may cause damage to biological macromolecules leading to severe health threats when they are present in high concentrations. Aromatic plants contain phytochemicals rich of antioxidants that prevent oxidant formation or scavenge oxidants produced under oxidative stress conditions. In the present study, we investigated the free radical scavenging effects, the antioxidant and ion toxicity preventive effect of Xylopia aethiopica (X. aethiopica), a plant of the family of Annonaceae used as spice in Cameroon. The scavenging properties of extracts of X. aethiopica were tested on 2, 2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), hydroxyl (OH), 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals. The total antioxidant capacity was assayed by ferric reducing ability of plasma (FRAP), phosphomolybdenum antioxidant power (PAP), reduction assays. The protective potential was carried on superoxide dismutase (SOD), catalase and peroxidases. The results showed that both the ethanolic (BEE) and the hydroethanolic (BEH) extracts from the barks of X. aethiopica scavenged all the tested radicals. The sample BEH showed the highest total antioxidant capacity both in the FRAP and the PAP. This result was positively correlated to its higher phenolic content (30.74±0.44 CAE/g dried extract). The higher protective capacity of BEH on SOD, catalase and peroxidase activities was comparable to that of the vitamin C used as standard. In conclusion, X. aethiopica has a higher antioxidant and protective potential against ion-mediated oxidative damage and may be considered as a potential drug against metal-mediated toxicity.

Zanthoxylum heitzii Modulates Ferric Nitrilotriacetate‐Dependent Oxidative Alterations in Four Vital Organs: An In Vitro Organoprotective Model

“…That molecule can be of enzymatic origins such as the SOD, catalase, and peroxidase [ 10 , 12 ]. This study has shown a significant variation of the activity of antioxidant enzymes from one organ to another corroborating previous authors who demonstrated the disparities in the antioxidant response between different vital organs [ 14 ]. The variation of the concentration of antioxidant enzymes in biological samples has been used in a diversity of study as a marker of oxidative stress [ 33 ].…”

“…The mixture was then centrifuged at 5000 rpm for 30 min [ 9 ]. The supernatant was then collected and kept in a fridge at 4°C for further experiments [ 14 ].…”

“…The obtained solution was then mixed to an H 2 O 2 , 200 mM 1 : 1 (v/v) (Fisher Scientific, USA). This oxidizing solution was used immediately after preparation [ 14 , 20 ].…”

“…A volume of 20 µ L of sample was added to a test tube containing 1 mL of Ellman's reagent (5-5′-dithio-bis(2-nitrobenzoic acid) in phosphate buffer pH 6.6, 0.1 M). The mixture was then homogenized and incubated at room temperature for three h. Then the OD were reassured at 412 nm, and the results were expressed in µ M/L using the formula OD = ε · C · l ; ε = 13600 [ 14 ].…”

Phenolic Compounds from Water-Ethanol Extracts ofTetrapleura tetrapteraProduced in Cameroon, as Potential Protectors againstIn VivoCCl4-Induced Liver Injuries

“…Polyphenolic compounds, widely distributed in plants, are known to have multiple biological effects such as anti-inflammatory, antidiabetic, antioxidant, and hepatoprotective [23, 24]. Recently, more attention has been given to plants which contain this class of compounds, with more emphasis on those used in the human diet [25, 26]. The fruit of T. tetraptera is a spice used as a condiment in Cameroon.…”