In vitro Antioxidant and Cytotoxic Effects of Methanol Extracts of Vitex negundo, Lantana camara, Bauhinia variegata and Barringtonia racemosa on Human Cancer Cell lines

Abstract

In current study, the phytochemical, antioxidant and cytotoxic effects of methanol extracts of Vitex negundo, Lantana camara, Bauhinia variegata and Bauhinia racemosa were reported. Methanol extract of medicinal plants (V. negundo, L. camara, B. variegata and B. racemosa) were extracted using different extraction methods and then tested for their antioxidant activity by 2,2-diphenylpicrylhydrazyl, superoxide scavenging method, chelation of metal ions and cytotoxic potency using MTT assay on human cancer cell lines. In different extraction methods, highest yield was obtained with hot extraction method. Total phenolic and flavonoid contents were found higher in methanol extract of V. negundo having 173.3±1.54 mg gallic acid equivalent/g DW and 24.41±1.5 mg quercetin equivalent/g DW contents, respectively. Leaves of V. negundo have maximum IC 50 value of 73±2.76 µg/ml and 350±1.23 µg/ml in 2,2-diphenylpicrylhydrazyl and iron chelating activity compared to all other methanol extracts. B. racemosa leaves have higher superoxide scavenging effect with IC 50 value of 223±1.23 µg/ml. V. negundo and L. camara leaves showed pronounced cytotoxic effect against HELA and KB human cancer cell lines with LD 50 value of 222±3.35 and 188.69±1.4 µg/ml, respectively. The present study shows that V. negundo and L. camara may be a probable source of natural antioxidant and as an anticancer drugs.

Effect of solvents and extraction methods on the phenolic content, flavonoid content, and antioxidant activity of Bauhinia variegata and Leptadenia reticulata

“…Linn, the highest total phenolic and Bauhinia variegata flavonoid content was observed in methanol extracts whereas water extract had lowest amount of phenolics. As per the study by Badgujar et al, (2017), the phenolic and flavonoid content in the methanol extracts of leaves were 112.0 ± 2.43 mg GAE/g and 20.73 ± 1.43 mg QE/g dry extract. In the same study, the IC 50 value of methanol extract for DPPH radical scavenging activity was reported to be 117±3.33µg/ml (Badgujar et al, 2017).…”

“…As per the study by Badgujar et al, (2017), the phenolic and flavonoid content in the methanol extracts of leaves were 112.0 ± 2.43 mg GAE/g and 20.73 ± 1.43 mg QE/g dry extract. In the same study, the IC 50 value of methanol extract for DPPH radical scavenging activity was reported to be 117±3.33µg/ml (Badgujar et al, 2017). This is in agreement with our study where methanol extracts were found to possess high amount of phenolics and flavonoids as well as free radical scavenging property in comparison to extracts prepared using other solvents.…”

Specific analytical methods for the extraction of common phytochemical constituents of Vitex negundo Linn: A mini-review

“…Maceration [32] Leaves -Methanol -Phenols (constituents not stated) -Flavonoids (constituents not stated) Percolation [42] Leaves -Ethanol -Flavonoids (constituents not stated)…”

“…extracted negundoside utilizing the solvent ethanol from the leaves of the sample. In the study "In vitro Antioxidant and Cytotoxic Effects of Methanol Extracts of Vitex negundo, Lantana camara, Bauhinia variegata and Bauhinia racemosa on Human Cancer Cell lines" by Badguhar et al [32] , V. negundo Linn leave extracts from cold maceration were phenols and flavonoids utilizing the solvent methanol.…”

Review On: Medicinal Uses, Phytochemistry And Pharmacological Action Of The Plant Bauhinia Racemosa (Lam.).

“…In India liver, lungs, breast, rectum, colon and stomach cancers are frequently detected. [10] Fig. 1.0 -Bauhinia racemosa (Lam.…”