In vitro antioxidant and pharmacognostic studies of leaf extracts of cajanus cajan (l.) millsp

Abstract

Mahitha, et al.: Antioxidant and Pharmacognostic Studies of Cajanus cajan Leaf Extracts Cajanus cajan (L.) Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC 50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested.The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

Phytochemical analysis and in vitro antioxidant stress properties of methanol acetate and ethyl extracts of Tirmania Nivea and Tirmania Pinoyi

“…The ferrous form can be measured by spectrophotometer at 700 nm. It has been shown in the literature that anti-radical properties are possible by adding a hydrogen atom that is capable of breaking the chain of free radicals (Gordon, 1990;Ramaswamy et al, 2015). The reducing power of the extracts depended on the concentration.…”

Cajanus cajan Leaves and Ginger Increase Antioxidant Defence in Pancreatic Diabetic Rats: An Immunohistochemical Study

“…Methanolic, ethanolic, and aqueous extracts of Cajanus cajan leaves exhibited antioxidant, hypoglycemic, and alphaglucosidase inhibitory activities (Aja et al, 2015;Mahitha et al, 2015;Wresdiyati et al, 2020a;Tungmunnithum et al, 2021). Aqueous and methanolic extracts of ginger extract were also reported to exhibit antioxidant and hypoglycemic activities (Wresdiyati and Astawan, 2004;Wresdiyati et al, 2007;Okafor and Okafor, 2022).…”