In vitro antioxidant and, α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans

Alam, Md. Monjurul; Zaidul, I.S.M.; Ghafoor, Kashif; Ferdosh, Sahena; Hakim, M. A.; Rafii, Mohd Y.; Abir, H M; Bostanudin, Mohammad F.; Perumal, Vikneswari; Khatib, Alfi

doi:10.1186/s12906-017-1684-5

Cited by 43 publications

(44 citation statements)

References 36 publications

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“…The phenolic compounds, acting as hydrogen donors, ROS scavengers, and reducing agents, are responsible for many biological activities such as hepatoprotective, anti-allergic, anticancer, anti-inflammatory, antimutagenic, antioxidant, and antidiabetic effects. 35 In the present work, EE had the highest total phenolic content, with 44.42±1.22 mg GAE/g dry weight.…”

Section: Discussionsupporting

confidence: 53%

Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of <i>Vaccinium arctostaphylos</i> L.

Barut

Engin

et al. 2019

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Bu çalışmanın amacı Vaccinium arctostaphylos L.'den hazırlanan etanol (EE), metanol (ME) ve su (AE) ekstraktlarının toplam fenolik, antosiyanin, flavonoit içerikleri ve biyolojik özelliklerinin incelenmesidir. Gereç ve Yöntemler: V. arctostaphylos'un EE, ME ve AE ekstraktları hazırlanmıştır. Bu ekstraktların total fenolik, antosiyanin ve flavonoid içerikleri, antioksidan (2,2'-difenil-1-pikrilhidrazil, metal iyon şelatlama ve ferrik indirgeyici antioksidan gücü metotları), α-glukozidaz, anti-inflamatuvar ve DNA koruma özellikleri araştırılmıştır. Bulgular: EE, 44.42±1.22 mg galik asit eşdeğeri/g kuru ağırlık, 8.46±0.49 mg/siyanidin-3-glukozid eş değerleri/g kuru ağırlık ve 9.22±0.92 mg quercetin eş değerleri/g kuru ağırlık değerleriyle en yüksek toplam fenolik, antosiyanin ve flavonoid içeriğine sahip olduğu görülmüştür. Bununla birlikte ekstraktların antioksidan aktiviteleri sırasıyla EE>ME>AE olduğu belirlendi. EE ve ME α-glukozidaz enzimini sırasıyla 0.301±0.002 mg/mL ve 0.477±0.003 mg/mL IC 50 değerleriyle inhibe etmiştir. Ayrıca, EE ve ME'nin inhibisyon sabiti (K i) değerleri 0.48±0.02 mg/mL ve 0.46±0.01 mg/mL bulunarak, yarışmasız inhibisyon gerçekleştirdikleri belirlenmiştir. EE'nin 100 ve 300 mg/kg dozları farelerde formalin ile indüklenen ödemi önemli derecede azalttığı belirlenmiştir. DNA koruma çalışmalarında, ekstraktlar radikal süpürme aktivitesinden dolayı Fenton reaktifiyle oluşturulan hasara karşı süpersarmal plasmid pBR322 DNA'yı korumuştur. Sonuç: Sonuçlarımız, V. arctostaphylos L.'nin EE'sinin güçlü antioksidan, anti-inflamatuvar, α-glukozidaz inhibisyon ve DNA koruyucu etkilere sahip olduğunu göstermiştir; bu, oksidatif hasar ve iltihaplanma ile ilişkili hastalıkları önlemek veya tedavi etmek için etkili bir tıbbi bitki olabileceğini düşündürmektedir. Anahtar kelimeler: Antioksidan, anti-inflamatuvar, DNA, α-glukozidaz, Vaccinium arctostaphylos Objectives: The scope of this study was to investigate the total phenolic, anthocyanin, and flavonoid contents and the biological properties of ethanol extract (EE), methanol extract (ME), and aqueous extract (AE) from Vaccinium arctostaphylos L. Materials and Methods: EE, ME, and AE of V. arctostaphylos were prepared. Various biological activities such as total phenolic, anthocyanin, and flavonoid contents, and antioxidant (2,2'-diphenyl-1-picrylhydrazyl ferrous ion-chelating, and ferric reducing antioxidant power assays), α-glucosidase inhibitory, anti-inflammatory, and DNA protective properties of these extracts were studied. Results: EE exhibited the highest total phenolic, anthocyanin, and flavonoid contents with 44.42±1.22 mg gallic acid equivalents/g dry weight, 8.46±0.49 mg/Cyaniding-3-glucoside equivalents/g dry weight, and 9.22±0.92 mg quercetin equivalents/g dry weight, respectively. The antioxidant activities of the extracts followed the order: EE>ME>AE. EE and ME inhibited α-glucosidase enzyme and their IC 50 values were 0.301±0.002 mg/mL and 0.477±0.003 mg/mL, respectively. In addition, EE and ME were determined as noncompetitive inhibitor...

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Section: Discussionsupporting

confidence: 53%

Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of <i>Vaccinium arctostaphylos</i> L.

Barut

Engin

et al. 2019

tjps

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“…(35). In this work, EE had the highest total phenolic content with 44.42 ± 1.22 mg GAE/ g dry weight.…”

Section: Discussionsupporting

confidence: 48%

Investigation of antioxidant, α-glucosidase inhibitory, anti-inflammatory and DNA protective properties of Vaccinium arctostaphylos L.

Barut¹,

Barut²,

Engin³

et al. 2018

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GİRİŞ ve AMAÇ:Bu çalışmanın amacı Vaccinium arctostaphylos (V. arctostaphylos) L.'den hazırlanan etanol (EE), metanol (ME) ve su (AE) ekstraktlarının toplam fenolik, antosiyanin, flavonoit içerikleri ve biyolojik özelliklerinin incelenmesidir. YÖNTEM ve GEREÇLER: V. arctostaphylos'un EE, ME ve AE ekstraktları hazırlandı. Bu ekstraktların total fenolik, antosiyanin ve flavonoid içerikleri, antioksidan (DPPH, metal iyon şelatlama ve FRAP metotları), α-glukozidaz, anti-inflamatuvar ve DNA koruma özellikleri araştırılmıştır. BULGULAR: EE, 44.42 ± 1.22 mg GAE/g kuru ağırlık, 8.46 ± 0.49 mg/CGE/g kuru ağırlık ve 9.22 ± 0.92 mg QEE/g kuru ağırlık değerleriyle en yüksek toplam fenolik, antosiyanin ve flavonoid içeriğine sahip olduğu görülmüştür. Bununla birlikte ekstraktların antioksidan aktiviteleri sırasıyla EE > ME > AE olduğu belirlendi. EE ve ME α-glukozidaz enzimini sırasıyla 0.301 ± 0.002 mg/mL ve 0.477 ± 0.003 mg/mL IC50 değerleriyle inhibe etmiştir. Ayrıca, EE ve ME'nin inhibisyon sabiti (Ki) değerleri 0.48 ± 0.02 mg/mL ve 0.46 ± 0.01 mg/mL bulunarak, yarışmasız inhibisyon gerçekleştirdikleri belirlenmiştir. EE'nin 100 ve 300 mg/kg dozları farelerde formalin ile indüklenen ödemi önemli derecede azalttığı belirlenmiştir. DNA koruma çalışmalarında, ekstraktlar radikal süpürme aktivitesinden dolayı Fenton reaktifiyle oluşturulan hasara karşı süpersarmal plasmid pBR322 DNA'yı korumuştur. TARTIŞMA ve SONUÇ: Sonuçlarımız, V. arctostaphylos L.'nin EE'sinin güçlü antioksidan, anti-inflamatuar, α-glukozidaz inhibisyon ve DNA koruyucu etkilere sahip olduğunu göstermiştir; bu, oksidatif hasar ve iltihaplanma ile ilişkili hastalıkları önlemek veya tedavi etmek için etkili bir tıbbi bitki olabileceğini düşündürmektedir.Anahtar Kelimeler: antioksidan, anti-inflamatuvar, DNA, α, -glukozidaz, V. arctostaphylos. INTRODUCTION:The scope of this study was to investigate total phenolic, anthocyanin, flavonoid contents and biological properties of ethanol extract (EE), methanol extract (ME) and aqueous extract (AE) from Vaccinium arctostaphylos (V. arctostaphylos) L. METHODS: EE, ME and AE of V. arctostaphylos were prepared. Various biological activities such as total phenolic, anthocyanin and flavonoid contents, antioxidant (DPPH, u n c o r r e c t e d p r o o f ferrous ion-chelating and FRAP assays), α-glucosidase inhibitory, anti-inflammatory and DNA protective properties of these extracts were studied. RESULTS: EE exhibited the highest total phenolic, anthocyanin and flavonoid contents with 44.42 ± 1.22 mg GAE/g dry weight, 8.46 ± 0.49 mg/CGE/g dry weight and 9.22 ± 0.92 mg QEE/g dry weight, respectively. The antioxidant activities of extracts followed order: EE > ME > AE. EE and ME inhibited α-glucosidase enzyme and their IC50 values were 0.301 ± 0.002 mg/mL and 0.477 ± 0.003 mg/mL respectively. In addition, EE and ME were determined as noncompetitive inhibitor with the inhibitory constant (Ki) values of 0.48 ± 0.02 mg/mL and 0.46 ± 0.01 mg/mL. 100 and 300 mg/kg doses of EE caused a significant reduction in formalin-induced ede...

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“…The columns situated opposite to the IC 50 (Y variable) column represent the ions (X variables) that are positively correlated with the activity [11]. The mass spectra of the ions of interest were compared to those in the NIST14 reference library, and only the metabolites with matching index more than 70% were considered [30]. In this study, 16 metabolites were identified to be responsible for the α-glucosidase inhibitory activity ( Figure 5).…”

Section: Putative α-Glucosidase Inhibitory Metabolitesmentioning

confidence: 99%

Investigation of α-Glucosidase Inhibitory Metabolites from Tetracera scandens Leaves by GC–MS Metabolite Profiling and Docking Studies

et al. 2020

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Stone leaf (Tetracera scandens) is a Southeast Asian medicinal plant that has been traditionally used for the management of diabetes mellitus. The underlying mechanisms of the antidiabetic activity have not been fully explored yet. Hence, this study aimed to evaluate the α-glucosidase inhibitory potential of the hydromethanolic extracts of T. scandens leaves and to characterize the metabolites responsible for such activity through gas chromatography–mass spectrometry (GC–MS) metabolomics. Crude hydromethanolic extracts of different strengths were prepared and in vitro assayed for α-glucosidase inhibition. GC–MS analysis was further carried out and the mass spectral data were correlated to the corresponding α-glucosidase inhibitory IC50 values via an orthogonal partial least squares (OPLS) model. The 100%, 80%, 60% and 40% methanol extracts displayed potent α-glucosidase inhibitory potentials. Moreover, the established model identified 16 metabolites to be responsible for the α-glucosidase inhibitory activity of T. scandens. The putative α-glucosidase inhibitory metabolites showed moderate to high affinities (binding energies of −5.9 to −9.8 kcal/mol) upon docking into the active site of Saccharomyces cerevisiae isomaltase. To sum up, an OPLS model was developed as a rapid method to characterize the α-glucosidase inhibitory metabolites existing in the hydromethanolic extracts of T. scandens leaves based on GC–MS metabolite profiling.

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In vitro antioxidant and, α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans

Cited by 43 publications

References 36 publications

Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of <i>Vaccinium arctostaphylos</i> L.

Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of <i>Vaccinium arctostaphylos</i> L.

Investigation of antioxidant, α-glucosidase inhibitory, anti-inflammatory and DNA protective properties of Vaccinium arctostaphylos L.

Investigation of α-Glucosidase Inhibitory Metabolites from Tetracera scandens Leaves by GC–MS Metabolite Profiling and Docking Studies

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