In vitro Antioxidant, Antimicrobial and in vivo Peripheral Analgesic Activities of Methanol and Petroleum Ether Extracts of Whole Plant of Uraria lagopoides DC

Hossain, Md. Monir; Uddin, Nizam; Uddin, Mohammad B.; Chowdhury, Arfia

doi:10.9734/bjpr/2015/8884

Cited by 2 publications

(2 citation statements)

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“…The total phenolic content of the leaf extract was determined according to the method outlined by Velioglu et al Similarly, determination of total flavonoid content was accomplished by using the aluminum chloride colorimetric method described by Jiao and Wang . On the other hand, the total antioxidant capacity was evaluated by following the phosphomolybdenum method described by Hossain and co-workers . Briefly, to test tube sample solutions of different concentrations were added experimental reagents and mixed well.…”

Section: Methodsmentioning

confidence: 99%

“…59 On the other hand, the total antioxidant capacity was evaluated by following the phosphomolybdenum method described by Hossain and co-workers. 60 Briefly, to test tube sample solutions of different concentrations were added experimental reagents and mixed well. Then, the test tubes were incubated to complete the reaction, and absorbance of each solution was measured with a Shimadzu UV PC-1600 spectrophotometer against the blank.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

Mondal

Hossain

Rahman

et al. 2019

Chem. Res. Toxicol.

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In folk medicines, Justicia gendarussa (J. gendarussa) is used as a depurative herb for treating fever, pain, and cancer and as laxative for constipation. The aim of the present investigation was to evaluate the hepatoprotective effect of the leaf methanol extract of J. gendarussa leaf (J gMe) against carbofuran (CF)-intoxicated liver injuries in Sprague−Dawley rats, along with the antioxidant activity of this extract. For this purpose, levels of serum diagnostic markers, hepatic antioxidant enzymes, and liver histoarchitecture were employed to justify the protective efficacy of J gMe. In addition, the phenolic and flavonoid contents of the extract were quantified, and antioxidant activity was investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide, and hydroxyl free radical scavenging assays. Results revealed that the leaf extract caused a significant (<0.05, <0.01) decrease of the level of hepatic enzymes, triglycerides, and bilirubin and an increase of the total protein. J gMe has also significantly (<0.05, <0.01) lowered the level of malonylaldehyde. Carbofuran markedly suppressed hepatic antioxidant enzymes, however, the leaf extract significantly augmented these enzymes. The hepatoprotective effect was demonstrated by the improvement in the histo-architectural features of liver sections of CFintoxicated rats treated with J gMe at 500 mg/kg dose. In addition, J gMe showed moderate total phenolic and total flavonoid content, whereas the IC 50 values of DPPH, nitric oxide, hydrogen peroxide, and hydroxyl free radical scavenging assays were 71.31 ± 0.42, 134.82 ± 0.14, 47.69 ± 0.38, and 118.44 ± 0.30 μg/mL, respectively. In conclusion, the present study suggests the protective role of J gMe against hepatic injury induced by CF, which may be attributed to its higher antioxidant properties and thereby scientifically justifies its traditional use.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

Mondal

Hossain

Rahman

et al. 2019

Chem. Res. Toxicol.

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Ethnomedicinal practices and phytochemical assessment of Uraria lagopoides (L.) DC. around Mayurjharna Reserve, Eastern India

Sanyal¹,

Bala²,

Mazumdar³

2017

J. Med. Plants Res.

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Plants have been used from ancient times in India for various systems of medicine like ayurveda, unani, homoeopathy, allopathy, siddha, ethnic, etc., to attempt cares for diseases and to relieve physical and mental sufferings. The aim of the study was to ascertain multiple usages of Uraria lagopoides in the outskirts of Mayurjharna and presence of phytochemicals in leaves and roots through standard tests for metabolites by extracting methanolic and aqueous solutions. Flavonoid and phenolic content in the extract were also determined by UV-visible spectrophotometer. U. lagopoides, a trailing perennial herb, was found to be the most important plant by the Santhal, Munda and Lodha ethnic community with a 'use value' of 1.85. Intensive exploration of U. lagopoides affirms that the plant has multipurpose use against various diseases viz. wound healing, anti-inflamatory, anti-diarrhoea, abortifacient, laxative, aphrodisiac and others. The methanolic extract reveals the presence of tannins, alkaloids, glycosides, carbohydrates, flavonoids, steroids, and saponins whereas aqueous extract shows positivity of tannins, glycosides, carbohydrates, flavonoids, and saponins. The flavonoid contents in U. lagopoides leaves and roots were 145.68 (±5.80) and 178.93 (±0.05) μg of quercetin per mg of dry extract, respectively. The phenolic content in leaves and roots were 43.073 (±1.36) and 40.195 (±2.13) μg of pyrrocatechol equivalent per mg of dry extract. Preliminary qualitative and quantitative screening confirms the presence of multiple metabolites which also commensurate the multiple usage of the roots and leaves of U. lagopoides.

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In vitro Antioxidant, Antimicrobial and in vivo Peripheral Analgesic Activities of Methanol and Petroleum Ether Extracts of Whole Plant of Uraria lagopoides DC

Cited by 2 publications

References 48 publications

Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

Hepatoprotective and Antioxidant Activities of Justicia gendarussa Leaf Extract in Carbofuran-Induced Hepatic Damage in Rats

Ethnomedicinal practices and phytochemical assessment of Uraria lagopoides (L.) DC. around Mayurjharna Reserve, Eastern India

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