In vitro antioxidant capacity of tea of Echinodorus grandiforus, “leather hat,” in Wistar rat liver

Abstract

Oxidative stress has been considered as one of the factors responsible for hepatic diseases, which sometimes require new ways of treatment. The present study aimed to evaluate the in vitro antioxidant capacity of the tea of Echinodorus grandiforus ("leather hat" plant) in rat liver. Different preparations of tea were evaluated for phenolic composition, antioxidant activity by DPPH assay and ability to inhibit lipid peroxidation induced by copper sulfate. The antioxidant activity was assessed in liver tissue treated with sodium azide in the presence or absence of tea by assays for lipid peroxidation (TBARS), protein oxidation (carbonyl) and the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). The results show that different preparations of tea are important sources of polyphenols and contain theobromine, catechin and vitexin. Furthermore, the results indicate that this tea exhibits an antioxidant activity by its ability to scavenge DPPH radical. Different preparations of tea prevented damage to lipids and proteins induced by sodium azide, as well as assisting in restoring CAT and SOD activities. Thus, it can be seen that E. grandiforus tea had antioxidant activity in serum and liver being able to prevent oxidative damages generated by sodium azide.

Avaliação da capacidade antioxidante e antiglicante de plantas medicinais e seu potencial de inibição das enzimas digestivas relacionadas ao diabetes mellitus tipo 2

“…It is well documented that isoorientin (Lunagariya et al, 2014) and isovitexin inhibit lipase activity, which justifies the pancreatic lipase inhibitory activity of E. grandiflorus EtOH extract. In addition, our results corroborate another study that showed that the tea from E. grandiflorus has anti-oxidant capacity (Lunardi et al, 2014).…”

“…The ethanolic extracts of C. sinensis, R. officinalis leaves and aqueous extract of E. grandiflorus leaves have also been reported to decrease hyperglycemia, oxidative stress, lipid peroxidation and activate antioxidant enzymes (Alonso-Castro et al, 2012;Haidari et al, 2013;Lunardi et al, 2014). A previous study also showed that M. charantia leaves control postprandial hyperglycemia and glucose tolerance, and its methanolic ex-tract presents free radical-scavenging properties and inhibitory activity against nitric oxide production (Tsai et al, 2014).…”

“…Os extratos etanólicos das folhas de chá-verde e de alecrim, e também o extrato aquoso de folhas de chapéu de couro, demonstraram ser capazes de diminuir a hiperglicemia, o estresse oxidativo, a peroxidação lipídica e ativar enzimas antioxidantes (ALONSO-CASTRO et al, 2012;HAIDARI et al, 2013;LUNARDI et al, 2014). A literatura também mostrou que o melão de São Caetano controla a hiperglicemia pós-prandial e auxilia no combate da tolerância à glicose, e seu extrato metanólico apresenta propriedades de eliminação de radicais livres e atividade inibitória contra a produção de óxido nítrico (TSAI et al, 2014).…”