In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from Tetragonula sapiens

Farida, Siti; Pratami, Diah Kartika; Sahlan, Muhamad; Laksmitawati, Dian Ratih; Rohmatin, Etin; Situmorang, Herbert

doi:10.1016/j.sjbs.2021.12.034

Cited by 15 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of TPC (10.017 to 17.043 mgGAE/g) and TFC (0.204 to 0.830 mgQE/g) in the present study were higher than the data reported by other researchers from Malaysia (TPC = ~0.697 µgGAE/g and TFC = ~7.437 µgQE/g) [ 38 ]. However, the results were lower than the data reported by researchers from Brunei (TPC = 2192.7 to 2391.0 mgGAE/g and TFC = 275.2 to 299.4 mgQE/g) [ 12 ] and Indonesia (TPC = 98.082 mgGAE/g and TFC = 15.890 mgQE/g) [ 39 ]. They also sourced their propolis samples from stingless bees, but they performed different extraction methods using a higher concentration of ethanol (80–96%).…”

Section: Resultscontrasting

confidence: 61%

Evaluating Biological Properties of Stingless Bee Propolis

Lim

Chua

Dawood

2023

Foods

View full text Add to dashboard Cite

The aim of the present study was to determine the content of phenolics, flavonoids and tannins, as well as the biological functions of propolis extracts from the stingless bee (Heterotrigona itama). The raw propolis was extracted via maceration with ultrasonic pretreatment in 100% water and 20% ethanol. The yield of ethanolic propolis extracts was about 1% higher than its aqueous counterpart. The colorimetric assays showed that the ethanolic propolis extract had about two times higher phenolics (17.043 mg GAE/g) and tannins (5.411 mg GAE/g), and four times higher flavonoids (0.83 mg QE/g). The higher phenolic content had enhanced the antiradical and antibacterial capacities of the ethanolic extract. The propolis extracts significantly exhibited higher antibacterial activity against gram-positive bacteria (Staphylococcus aureus) than gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). However, aqueous extract was found to have a higher anticancer property based on the viability of lung cancer cells. No cytotoxic effect was observed on normal lung cells as the cell viability was maintained >50%, even the concentration of propolis extracts were increased up to 800 µg/mL. Different chemical compositions of propolis extract would show different bioactivities depending upon the individual applications. The high content of phenolics suggests that the propolis extract could be a natural source of bioactive ingredients for the development of innovative and functional foods.

show abstract

Section: Resultscontrasting

confidence: 61%

Evaluating Biological Properties of Stingless Bee Propolis

Lim

Chua

Dawood

2023

Foods

View full text Add to dashboard Cite

show abstract

“…South Sulawesi propolis has a FRAP antioxidant level of 32.1 µg/ml (Pratami, et al, 2018). Propolis from other parts of Indonesia derived from Tetragonula bees has a FRAP antioxidant activity of 189.05 mg/g (Farida, et al, 2022) The use of highpower levels and hexane as solvent in the extraction process produces extracts with a temperature of 60 °C which is the ideal temperature to maintain the content of active ingredients that are antioxidants in propolis. The FRAP methodology was carried out to determine the ability of a substance to bind iron ions from oxidation (Kumar, 2015).…”

Section: Antioxidant Level Frap Methodsmentioning

confidence: 99%

EVALUATION ON ANTIOXIDANT ACTIVITY AND ACTIVE COMPONENTS OF TRIGONA ITAMA PROPOLIS EXTRACT AND ITS POTENTIAL AS SARS-CoV2 INFECTION INHIBITORS

Putri Primandasari,

Susilo,

Umam Al Awwaly

et al. 2023

JTP

View full text Add to dashboard Cite

Propolis Trigona itama has a blackish brown color and has a bitter taste. Propolis contains immunomodulator, antibacterial, antifungal and antiviral. Efforts made to determine the potential of propolis require an extraction process. The extraction process aims to obtain propolis ready for consumption. Propolis has thermostable properties with a melting point of 60oC – 65oC, so it does not require high temperatures in the extraction process. The extraction process using MAE requires a faster time because the pressure and temperature can be controlled. This study used a factorial completely randomized design with 2 factors with 9 treatments and 3 replications. The data obtained were analyzed by analysis of variant (ANOVA). If there is a difference, it is continued with the DUNCAN multiple distance test. The results of the extraction using the MAE method of propolis have antioxidant activity using the DPPH method of 1,460µg/ml to 1,413 µg/ml which is expressed in IC50. Trigona itama propolis extract had an average antioxidant value of 0.22 µg/ml to 0.57 µg/ml using the FRAP method.There is a unique component in the GCMS results of Trigona itama propolis extract called bisalcofen.Bisalcofen is an active component which has antioxidant properties. The results of the bisalcofen docking score of -7.6 on the ACE2 protein. Bisalcofen has a stronger potency than umifenovir which can be used as an inhibitor of SARS-CoV2 infection.

show abstract

“…Senyawa terpenoid tersebut telah banyak ditemukan dan terbukti memiliki efek antiinflamasi (34,35) . Beberapa senyawa terpenoid diketahui secara signifikan dapat menghambat perkembangan infl amasi sendi kronis dimana senyawa ini dapat mempengaruhi beragam mekanisme yang relevan dengan peradangan yang timbul sebagai respons terhadap berbagai faktor etiologi (36,37) . Kerusakan jaringan yang terjadi saat proses infl amasi yang disebabkan karena pengkerutan sel limfosit dapat dihambat oleh steroid glikosida dari ekstrak tanaman Trigonella foenum graecum (38) .…”

Section: Bahan Dan Metodeunclassified

Aktivitas Anti-inflamasi Parem Instant Tradisional dari Bahan Usada Bali pada Mencit Inflamasi yang Diinduksi Karagenan

Yuda¹,

Sasmita²,

Cahyaningsih³

2022

jifi

View full text Add to dashboard Cite

Maraknya efek samping penggunaan obat anti-inflamasi steroid dan non steroid yang digunakan dalam jangka panjang oleh pasien rematik memerlukan alternatif terapi yang relatif lebih aman. Penelitian ini bertujuan untuk mengetahui efektivitas parem instan dari Usada Bali yang diberi nama BIO-PAREM sebagai agen anti-inflamasi terhadap mencit inflamasi dengan diinduksi karagenan. Penelitian ini merupakan randomized pre-test and post-test with control group design dengan subjek 24 ekor mencit terbagi dalam empat kelompok perlakuan. Kelompok pertama sebagai kontrol negatif diberikan pembawa (aquadest), kelompok dua kontrol positif diberikan gel natrium diklofenak, kelompok tiga (P1) diberikan BIO-PAREM konsentrasi 12,5% dan kelompok 4 (P2) diberikan BIO-PAREM konsentrasi 25%. Seluruh kelompok diinduksi dengan karagenan 1% (b/v), kemudian diberikan perlakuan secara topikal dan kemudian dilakukan pengukuran perubahan volume peradangan kaki mencit menggunakan pletismometer pada jam ke-0, 1, 2, 3, dan 4. Uji Kruskal Wallis dan Mann Whitney (taraf kepercayaan 95%) digunakan untuk melakukan analisis terhadap perubahan volume peradangan relatif. Dari hasil pengujian skrining fitokimia BIO-PAREM baik pada konsentrasi 12,5% maupun 25% menunjukkan adanya kandungan senyawa tannin, steroid, triterpenoid, flavonoid dan alkaloid. BIO-PAREM pada kedua konsentrasi mampu menghambat inflamasi secara bermakna jika dibandingkan dengan kontrol negatif (p<0,05), namun kedua konsentrasi tersebut menghasilkan aktivitas antiinflamasi yang sebanding (p>0,05).

show abstract

In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from Tetragonula sapiens

Cited by 15 publications

References 50 publications

Evaluating Biological Properties of Stingless Bee Propolis

Evaluating Biological Properties of Stingless Bee Propolis

EVALUATION ON ANTIOXIDANT ACTIVITY AND ACTIVE COMPONENTS OF TRIGONA ITAMA PROPOLIS EXTRACT AND ITS POTENTIAL AS SARS-CoV2 INFECTION INHIBITORS

Aktivitas Anti-inflamasi Parem Instant Tradisional dari Bahan Usada Bali pada Mencit Inflamasi yang Diinduksi Karagenan

Contact Info

Product

Resources

About