In vitro antioxidant properties of calcium dobesilate

Abstract

Calcium dobesilate, a vascular protective agent, was tested in vitro for its scavenging action against oxygen free radicals. Calcium dobesilate was as potent as rutin to scavenge hydroxyl radicals (IC50 = 1.1 vs 0.7 microM, respectively). It was also able to scavenge superoxide radicals, but with 23 times less potency than rutin (IC50 = 682 vs 30 microM, respectively). Calcium dobesilate significantly reduced platelet activating factor (PAF)-induced chemiluminescence in human PMN cells and lipid peroxidation by oxygen free radicals in human erythrocyte membranes, although these actions required calcium dobesilate concentrations > or = 50 microM. Finally, in cultured bovine aortic endothelial cells, magnesium dobesilate reduced the increase in cytosolic free calcium induced by hydrogen peroxide and inhibited phenazine methosulfate-induced cell potassium loss. In conclusion, calcium dobesilate was effective in scavenging hydroxyl radicals in vitro, at therapeutically relevant concentrations. Conversely, higher concentrations of the compound were required to scavenge superoxide radicals or to protect the cells against the deleterious effects of intracellular reactive oxygen species. Further studies in vivo are required to determine if these antioxidant properties of calcium dobesilate can play a role in its vascular protective mechanisms.

Calcium Dobesilate Is Protective against Inflammation and Oxidative/Nitrosative Stress in the Retina of a Type 1 Diabetic Rat Model

“…Deeper insights into the mechanism of action of CaD showed the beneficial effects of this drug as a result of its antioxidant properties, reflected particularly against the hydroxyl radical [36] and also by preventing the decrease in cellular antioxidant glutathione [37] . Its protective effects against diabetes-induced endothelial dysfunction appear associated with the decrease in advanced glycation end product levels and consequently to its antiangiogenic properties [38] .…”

Memory Enhancing Effect of Ethanolic Extract of Stevia rebaudiana (Bert.)

“…It has been also observed that elderly patients suffering from Alzeimer's disease showed reduction in symptoms upon chronic use of anti-inflammatory drugs 22 . It have been reported that Stevia rebaudiana have anti-inflammatory and antioxidant activity [23][24] . These anti-inflammatory and antioxidant effects may reduce brain damage and improve the neuronal functions of brain.…”

Calcium dobesilate attenuates vascular injury and the progression of diabetic retinopathy in streptozotocin-induced diabetic rats

“…In addition, aortae from both normal and diabetic rabbits exposed to elevated concentrations of glucose in vitro demonstrate impairment of endotheliumdependent relaxation [43]. Therefore, the antioxidant properties of DOBE [17,18] and its action on NO synthesis [22] could together constitute an additional mechanism explaining the beneficial effect of DOBE in restoring endothelium-dependent relaxation in aortae from diabetic animals to normal.…”

“…The main effect of the drug is to reduce microvascular permeability [16], leading to improved visual acuity. The pharmacological activity may be explained in part by the antioxidant properties of DOBE [17,18], through its action on the endothelium, which increases NO synthesis [15], and endothelium-dependent relaxation [18]. On the other hand, there is a disagreement about the possible effect of diabetes mellitus on vasoconstriction [19,20].…”