In-vitro antiulcer activities of petal extract of Crocus sativus var. cashmerianus

Abstract

Medicinal plants have been known for millennia as a rich source of traditional therapeutic agents for the prevention of diseases and ailments. The aim of the present study was performed to evaluate the antiulcer activities of hydro-alcoholic extracts of petals of Crocus sativus var. Cashmerianus by in-vitro methods viz. acid neutralizing capacity and H+/K+ - ATPase inhibition activity. In acid neutralizing capacity method, the petals extract significantly reduced acidity to 6.10 at a concentration of 1000 mg/ml as compared to 11.90 with standard 500 mg/ml of Aluminium hydroxide + Magnesium hydroxide combination. However, H+/K+ - ATPase inhibition activity method, petals extract showed maximum percentage inhibition of 70.31 % at the concentration 400µg/ml as compared to 73.82 % with a similar dose of standard Omeprazole. The IC 50 value of petals extract of C. sativus var. cashmerianus is shown 100 µg/ml in comparison with standard omeprazole of 82.5 µg/ml. The study reveals that the petals extract of C. sativus var. cashmerianus may contain compounds possessing acid neutralize and enzyme inhibition activities, thus it can be used as an alternative medicine for gastrointestinal disorders.

Lupeol: A Triterpenoid Isolated from the Stem Bark of Hymenocardia Acida (tul.) Exhibits a van der Waal Antagonism on the Alpha Subunit of Gastric H+K+Atpase – A Promising Antiulcer Principle

“…Sodium bicarbonate (antacid A) and a combination of aluminum hydroxide and magnesium hydroxide (antacid B) were used as bioassay standards in the in vitro model. Artificial gastric acid was prepared following the standard method as described by 17 18 19…”

“…About 90 mL of each sample (crude extract, aqueous fraction, bulked vacuum liquid chromatography) were added to 100 mL of artificial gastric juices at pH 1.2. The change in pH values was determined to examine the neutralizing effect of the samples [19].…”

“…Sodium bicarbonate (antacid A) and a combination of aluminum hydroxide and magnesium hydroxide (antacid B) were used as bioassay standards in the in vitro model. Artificial gastric acid was prepared following the standard method as described by [17][18][19] In addition, pH determinations of about 90 mL each for all the test samples, negative control, and positive control were carried out at temperatures ranging from 25-37 °C.…”

UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL BIJI NANGKA (Artocarpus heterophyllus Lam.) TERHADAP STABILISASI MEMBRAN SEL DARAH MERAH

“…Larutan Alsever dibuat dengan cara mencampurkan 2 gram dekstrosa, 0,8 gram natrium sitrat, 0,05 gram asam sitrat dan 0,42 gram NaCl dan dilarutkan dalam aquadest sampai 100 ml, kemudian sterilisasi menggunakan autoclave pada suhu 115℃ selama 30 menit (Kumar et al, 2012).…”

In-vitro anti-inflammatory and antioxidant potential of Triphala guggul tablets

“…Anti-oxidants proceed as a major defense against free radical mediated toxicity, effective in the prevention and treatment of complex diseases like atherosclerosis, alzheimer disease, diabetes, cancer and stroke. Medicinal plants well known for millennia as a potential source of traditional therapeutic agents for the prevention of diseases and ailments [4]. Natural compounds like flavonoids and phenolic compounds are widely dispersed in medicinal plants which have been reported to large number of biological effects including antioxidants, anti-inflammatory and anticarcinogenic [5,6].…”