In vitro antiviral activity of fluoroquinolones against African swine fever virus

Mottola, Carla; Freitas, Ferdinando B.; Simões, Margarida; Martins, Carlos; Leitão, Alexandre; Ferreira, Fernando

doi:10.1016/j.vetmic.2013.01.018

Cited by 46 publications

(34 citation statements)

References 21 publications

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“…uncinatus . Similar report has been associated with increasing dose of other drugs tested against the virus [13,33,34]. The antiviral activities of this plant are comparable to those of Lauryl gallate and mulberry twigs which caused significantly reduced viral titres and inhibition of viral DNA replication at non-toxic doses [13,34].…”

Section: Discussionsupporting

confidence: 67%

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Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

et al. 2013

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BackgroundAfrican swine fever (ASF), a highly contagious fatal acute haemorrhagic viral disease of pigs currently has no treatment or vaccination protocol and it threatens the pig industry worldwide. Recent outbreaks were managed by farmers with ethnoveterinary preparations with various claims of effectiveness.ResultsWe identified 35 compounds using GC-MS protocol and ASF virus (NIG 99) was significantly reduced by some extracts and fractions of the plant. However, the plant was poorly extracted by water and cytotoxicity was found to be a major problem with the use of the plant since its extracts also reduced the primary cells used in the assay.ConclusionIt is confirmed that the plant has antiviral potentials against ASF virus and farmers’ claims seem to have certain degree of veracity, but finding the best means of exploring the potential of the plant while reducing its cytotoxic effect in-vitro and in-vivo will be necessary.

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Section: Discussionsupporting

confidence: 67%

“…The drug also caused altered viral protein possibly because of the putative ASFV-topoisomerase II enzyme which was targeted and had its activity modified [33]. Lauryl gallate has been tested and confirmed to strongly inhibit African swine fever virus at non-toxic concentration.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

et al. 2013

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“…Although P1192R was identified as having homology to type II topoisomerases (Baylis et al, 1992;García-Beato et al, 1992) and it has been suggested that type II topoisomerase-inhibiting drugs have an effect on ASFV (Mottola et al, 2013;Salas et al, 1983), proof of pP1192R functionality remained unaddressed. Heterologous expression of P1192R using either prokaryotic or eukaryotic systems had been proposed and/or attempted by different research groups but, to our knowledge, the proteins obtained have never been shown to possess type II DNA topoisomerase activity.…”

Section: Discussionmentioning

confidence: 99%

African swine fever virus ORF P1192R codes for a functional type II DNA topoisomerase

Coelho¹,

Martins²,

Ferreira³

et al. 2015

Virology

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Topoisomerases modulate the topological state of DNA during processes, such as replication and transcription, that cause overwinding and/or underwinding of the DNA. African swine fever virus (ASFV) is a nucleo-cytoplasmic double-stranded DNA virus shown to contain an OFR (P1192R) with homology to type II topoisomerases. Here we observed that pP1192R is highly conserved among ASFV isolates but dissimilar from other viral, prokaryotic or eukaryotic type II topoisomerases. In both ASFV/Ba71V-infected Vero cells and ASFV/L60-infected pig macrophages we detected pP1192R at intermediate and late phases of infection, cytoplasmically localized and accumulating in the viral factories. Finally, we used a Saccharomyces cerevisiae temperature-sensitive strain in order to demonstrate, through complementation and in vitro decatenation assays, the functionality of P1192R, which we further confirmed by mutating its predicted catalytic residue. Overall, this work strengthens the idea that P1192R constitutes a target for studying, and possibly controlling, ASFV transcription and replication.

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“…Here, we have reviewed potential molecular targets to be considered as targets for antivirals. Antivirals against ASFV described include resveratrol and oxyresveratrol [73], microalgae [74], cholesterol lowering drugs [46] or inhibitors of cholesterol transport [42], antitumoral lauryl-gallate [75], anticonvulsivant valproic acid [75], dynamin inhibitors [38], fluoroquinolones [76], serine protease inhibitors [52], specific peptides [77], and miscelanea [32]. These could be eventually applied in a quick response to reduce susceptible animals in order to create ‘safe areas’ around the outbreaks and thus control the spread of the infection.…”

Section: Potential Vaccines and Antiviralsmentioning

confidence: 99%

African Swine Fever Virus: A Review

Galindo

Alonso

2017

Viruses

468

326

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African swine fever (ASF) is a highly contagious viral disease of swine which causes high mortality, approaching 100%, in domestic pigs. ASF is caused by a large, double stranded DNA virus, ASF virus (ASFV), which replicates predominantly in the cytoplasm of macrophages and is the only member of the Asfarviridae family, genus Asfivirus. The natural hosts of this virus include wild suids and arthropod vectors of the Ornithodoros genus. The infection of ASFV in its reservoir hosts is usually asymptomatic and develops a persistent infection. In contrast, infection of domestic pigs leads to a lethal hemorrhagic fever for which there is no effective vaccine. Identification of ASFV genes involved in virulence and the characterization of mechanisms used by the virus to evade the immune response of the host are recognized as critical steps in the development of a vaccine. Moreover, the interplay of the viral products with host pathways, which are relevant for virus replication, provides the basic information needed for the identification of potential targets for the development of intervention strategies against this disease.

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In vitro antiviral activity of fluoroquinolones against African swine fever virus

Cited by 46 publications

References 21 publications

Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

African swine fever virus ORF P1192R codes for a functional type II DNA topoisomerase

African Swine Fever Virus: A Review

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