In vitro Argonaute cleavage-mediated quantitative PCR facilitates versatile CRISPR/Cas-induced mutant analysis

Wang, Yijie; Li, Rong; Zhu, Zaobing; Wang, Chen; Wang, Li; Zhang, Dabing; Yang, Lei

doi:10.1016/j.snb.2022.132781

Cited by 10 publications

(2 citation statements)

References 33 publications

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“…In comparison to earlier techniques, SMART has shown to be more adaptable in performing induced mutant identification, genotyping, and GEF assessment with improved specificity, sensitivity, and accuracy. 131 This demonstrates the potential for designing a biosensing platform that combines the CRISPR/Cas system and Ago protein, leading to further breakthroughs in pathogens detection.…”

Section: ■ Applications Of Mds-derived Biosensorsmentioning

confidence: 95%

“…In order to examine CRISPR/Cas-induced mutations, genotypes, and GEF (gene editing frequency), Wang et al offered a novel SMART technique that makes use of DNA-guided TtAgo in vitro cleavage-mediated qPCR, or digital PCR. In comparison to earlier techniques, SMART has shown to be more adaptable in performing induced mutant identification, genotyping, and GEF assessment with improved specificity, sensitivity, and accuracy . This demonstrates the potential for designing a biosensing platform that combines the CRISPR/Cas system and Ago protein, leading to further breakthroughs in pathogens detection.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

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CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

Yang,

Mao,

Wang

et al. 2023

ACS Sens.

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Over the past few decades, pathogens have posed a threat to human security, and rapid identification of pathogens should be one of the ideal methods to prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive and specific approaches to identify and quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems and Argonaute (Ago) belong to the Microbial Defense Systems (MDS). The guided, programmable, and targeted activation of nucleases by both of them is leading the way to a new generation of pathogens detection. We compare these two nucleases in terms of similarities and differences. In addition, we discuss future challenges and prospects for the development of the CRISPR/Cas systems and Argonaute (Ago) biosensors, especially electrochemical biosensors. This review is expected to afford researchers entering this multidisciplinary field useful guidance and to provide inspiration for the development of more innovative electrochemical biosensors for pathogens detection.

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Section: ■ Applications Of Mds-derived Biosensorsmentioning

confidence: 95%

Section: Challenges and Prospectsmentioning

confidence: 99%