In Vitro Assessment of Antibacterial Activity and Cytocompatibility of Silver-Containing PHBV Nanofibrous Scaffolds for Tissue Engineering

Abstract

Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5-13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanofibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.

Biosynthesis of nanoparticles and silver nanoparticles

“…In 2010, Xing et al deduced that (poly-(-3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV nanofiber scaffolds containing AgNPs have the tendency of aiding in bone and skin tissue regeneration from their extensive study on both osteoblast (bone cells) and fibroblast (skin cells) cultured on such scaffolds. Hence, the risk associated with implantation surgery can be overcome by fabricating the surface of structure of the bone implants devices and scaffolds with silver nanoparticles (Xing et al 2010).…”

Antibacterial and conductive injectable hydrogels based on quaternized chitosan-graft-polyaniline/oxidized dextran for tissue engineering

“…antibiotic [29] and silver [30], into scaffolds to solve this problem. However, with the appearance of multiple drug resistant strains, the problem of bacterial resistance is getting worse [31], and the scaffolds may also lose their antibacterial activity after the complete release of the antibacterial substances.…”

Cytocompatible 3D chitosan/hydroxyapatite composites endowed with antibacterial properties: toward a self-sterilized bone tissue engineering scaffold

“…First, a eukaryotic (animal) cell is usually larger than a prokaryotic cell, showing higher structural and functional redundancy; therefore, a higher silver ion concentration is required to achieve comparable toxic effects on a eukaryotic cell than on a bacterium [46]. Second, we believe that silver phosphate prepared by the ion exchange method has significant advantages when compared to common water-soluble silver salts and silver nanoparticles.…”