In vitro assessment of chelating agents with regard to their abstraction efficiency of Cd2+ bound to plasma proteins

Jahromi, Elham Zeini; Gailer, Jürgen

doi:10.1039/c2mt20084h

Cited by 8 publications

(8 citation statements)

References 58 publications

(97 reference statements)

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“…In addition, and in contrast to conventional proteomic approaches, sample preparation plays a much more critical role during the determination of metalloproteins in biological tissues, due to trace metal contamination issues and the possibility of compromising the integrity of the metal-protein bonds of the metalloproteins of interest. General considerations regarding the drawbacks of and solutions for this kind of analysis have been exhaustively reviewed [5,[167][168][169][170][171][172][173].…”

Section: Analytical Strategies and Techniques For Multielement Biologmentioning

confidence: 99%

Biological responses related to agonistic, antagonistic and synergistic interactions of chemical species

et al. 2012

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The fact that the essential or toxic character of elements is species specific has encouraged the development of analytical strategies for chemical speciation over the last twenty years; indeed, there are now a great number of them that provide very good performance. However, biological systems are exposed to a complex environment in which species of elements can interact in a synergistic/antagonistic fashion. Thus, the metabolism of trace elements cannot be considered in isolation. On the other hand, biological systems are dynamic, so it is necessary to study the trafficking of species of elements between organs, tissues or cell compartments in order to decipher the biochemical processes of the interactions in which they are involved. Although the application of liquid chromatography-inductively coupled plasma-based "metallomics" methods in combination with organic mass spectrometry can provide much-needed insight, new analytical strategies are required to really understand the role of species of elements in biological systems and the mechanisms of their interactions. In the present paper, the interactions of the most widely studied elements in this context (Se, Hg and As) are discussed, as well as other important interactions between different elements.

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Section: Analytical Strategies and Techniques For Multielement Biologmentioning

confidence: 99%

Biological responses related to agonistic, antagonistic and synergistic interactions of chemical species

et al. 2012

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“…(b) Displacement of Zn 2+ from a plasma metalloprotein by Cd 2+ [26]. (c) Binding of Cd 2+ to plasma proteins [26,36,111]. (d) Abstraction of Cd 2+ from plasma proteins by chelating agents (CA) [36].…”

Section: Interaction Of a Toxic Metal With A Single Ligandmentioning

confidence: 99%

“…(c) Binding of Cd 2+ to plasma proteins [26,36,111]. (d) Abstraction of Cd 2+ from plasma proteins by chelating agents (CA) [36]. (e) Comparative binding of cis-platin/carboplatin to plasma proteins [53].…”

Section: Interaction Of a Toxic Metal With A Single Ligandmentioning

confidence: 99%

“…Since the manipulation of the distribution of metal ions in biological organisms is a very complicated process (i.e., not very amenable to theoretical modeling [188]), one relies on empirical evidence from biological experiments to gain insight. This notion is exemplified by the recent application of SEC to systematically assess the efficiency of three chelating agents to abstract Cd 2+ from rabbit plasma proteins in vitro [36]. Using a Superdex 200 SEC column, PBS-buffer as the mobile phase and an ICP-AES, the analysis of rabbit plasma that had been spiked with 2.0 g Cd 2+ /mL revealed two distinct Cd-peaks, which were tentatively identified as 2 -macroglobulin and rabbit serum albumin.…”

Section: ''Top-down'' Lc Approaches To Probe the Interaction Of Toxicmentioning

confidence: 99%

“…Thus, the application of LC-based approaches has potential to provide insight into the etiology of hitherto elusive metal species related disease processes [34,35]. The third reason is the inherent ability that LC-based approaches offer in terms of developing more effective antidotes against specific metal species to ultimately advance human health [36,37]. As a result, the application of LC-based approaches will likely continue to play an important role in "metal species in biology" research in the years to come.…”

Section: Introductionmentioning

confidence: 99%

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Metal Species in Biology: Bottom-Up and Top-Down LC Approaches in Applied Toxicological Research

Gailer

2013

ISRN Chromatography

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Since the inception of liquid chromatography (LC) more than 100 years ago this separation technique has been developed into a powerful analytical tool that is frequently applied in life science research. To this end, unique insights into the interaction of metal species (throughout this manuscript “metal species” refers to “toxic metals, metalloid compounds, and metal-based drugs” and “toxic metals” to “toxic metals and metalloid compounds”) with endogenous ligands can be obtained by using LC approaches that involve their hyphenation with inductively coupled plasma-based element specific detectors. This review aims to provide a synopsis of the different LC approaches which may be employed to advance our understanding of these interactions either in a “bottom-up” or a “top-down” manner. In the “bottom-up” LC-configuration, endogenous ligands are introduced into a physiologically relevant mobile phase buffer, and the metal species of interest is injected. Subsequent “interrogation” of the on-column formed complex(es) by employing a suitable separation mechanism (e.g., size exclusion chromatography or reversed-phase LC) while changing the ligand concentration(s), the column temperature or the pH can provide valuable insight into the formation of complexes under near physiological conditions. This approach allows to establish the relative stability and hydrophobicity of metal-ligand complexes as well as the dynamic coordination of a metal species (injected) to two ligands (dissolved in the mobile phase). Conversely, the “top-down” analysis of a biological fluid (e.g., blood plasma) by LC (e.g., using size exclusion chromatography) can be used to determine the size distribution of endogenous metalloproteins which are collectively referred to as the “metalloproteome”. This approach can provide unique insight into the metabolism and the plasma protein binding of metal species, and can simultaneously visualize the dose-dependent perturbation of the metalloproteome by a particular metal species. The concerted application of these LC approaches is destined to provide new insight into biochemical processes which represent an important starting point to advance human health in the 21st century.

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Simultaneous observation of the metabolism of cisplatin and NAMI-A in human plasma in vitro by SEC-ICP-AES

et al. 2014

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Single drug-based cancer therapies are frequently associated with the development of drug resistance. To overcome this problem, combination therapy with two or more anticancer drugs is a promising strategy, but clinical studies are logistically challenging and costly. Intermediary in vitro studies, however, can provide critical insight to decide whether one should proceed to in vivo studies. To this end, cisplatin and the Ru-based anticancer drug NAMI-A were added to human plasma and the size distribution of Pt-containing and Ru-containing entities was determined over a 2 h period. The results revealed a dramatically different rate of plasma protein binding for each drug and/or their hydrolysis products. Both drugs bound to the same apparent plasma proteins, but crucially they did not adversely affect each other's metabolism. Therefore, combination therapy of patients with these metallodrugs should be further assessed in clinical studies in order to systematically develop an effective combination therapy protocol to prevent the resurgence of cancer.

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In vitro assessment of chelating agents with regard to their abstraction efficiency of Cd2+ bound to plasma proteins

Cited by 8 publications

References 58 publications

Biological responses related to agonistic, antagonistic and synergistic interactions of chemical species

Biological responses related to agonistic, antagonistic and synergistic interactions of chemical species

Metal Species in Biology: Bottom-Up and Top-Down LC Approaches in Applied Toxicological Research

Simultaneous observation of the metabolism of cisplatin and NAMI-A in human plasma in vitro by SEC-ICP-AES

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