In vitro assessment of cobalt oxide particle toxicity: Identifying and circumventing interference

Darolles, Carine; Sage, Nicole; Armengaud, Jean; Malard, Véronique

doi:10.1016/j.tiv.2013.04.008

Cited by 23 publications

(37 citation statements)

References 55 publications

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“…Nanoparticles themselves can interfere with many tests, and it is often necessary to adapt the protocol to obtain reliable results [134,135]. A standardization of toxicity protocols, long-term study of nanoparticle toxicity and the fate of these nanomaterials in human tissue and in the environment need to be further investigated.…”

Section: Final Remarksmentioning

confidence: 99%

Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

188

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This review discusses recent advances in the synthesis, characterization and toxicity of metal oxide nanoparticles obtained mainly through biogenic (green) processes. The in vitro and in vivo toxicities of these oxides are discussed including a consideration of the factors important for safe use of these nanomaterials. The toxicities of different metal oxide nanoparticles are compared. The importance of biogenic synthesized metal oxide nanoparticles has been increasing in recent years; however, more studies aimed at better characterizing the potent toxicity of these nanoparticles are still necessary for nanosafely considerations and environmental perspectives. In this context, this review aims to inspire new research in the design of green approaches to obtain metal oxide nanoparticles for biomedical and technological applications and to highlight the critical need to fully investigate the nanotoxicity of these particles. OPEN ACCESSMetals 2015, 5 935

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Section: Final Remarksmentioning

confidence: 99%

Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

188

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“…Similar investigations in the literature in-vitro increasingly are finding that once effective endpoints and metrics of toxic interactions with lung cells are found, the epidemiological assumptions about toxicity as a function best predicted by particle size and inhaled mass are inadequate to explain toxicity 222 . The wide range of toxic interactions requires comprehensive analysis of biological endpoints, while accounting for adsorbed toxicants and immunological interactions as well as sizedistribution 163 .…”

Section: Discussionmentioning

confidence: 78%

“…Methods of circumventing particulate-assay interference centre on lowering concentrations, identifying when interference occurs during the assay procedure and appropriate centrifugation and media-transfer steps 165,222 . …”

Section: Assay Considerations and Particulate Interferencementioning

confidence: 99%

“…The interference of particulate matter in the proper functioning of in-vitro assays is quickly becoming a recognized problem 165,166,222 , however the development of mitigation strategies is still to gain traction in nano and micron-size particle in-vitro research. Failure to take interference into account leads to problems, in the form of overestimated toxicity in formazan MTT assays and underestimation in Lactate Dehydrogenase (LDH) assays 166 .…”

Section: Cell Proliferationmentioning

confidence: 99%

“…Concern over the toxicity of the metal species content in urban PM10 is also reflected in epidemiological data 259 . between different assays and either exacerbate or reduce perceived particle toxicity unless accounted for 166,222 . Particular care was taken to adapt colorimetric cytotoxicity assays to avoid particle interference, as guaranteeing the similar cytotoxicity profiles between different endpoints is essential to their calibration and interpretation.…”

Section: Introductionmentioning

confidence: 99%

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In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

Prakash¹

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This project investigated the cytotoxicity profiles and toxicological properties of inhalable iron-rich particles generated by mining activity. National Environment Protection Measure (NEPM) ambient air quality regulations specifically regulate sub-10 µm (PM10) and sub-2.5 µm (PM2.5) diameter particle fractions corresponding to thoracic and respirable lung penetration, respectively. However, atmospheric concentrations of fugitive iron-rich particulates in iron ore mining and processing associated settlements raised concerns that NEPM limits may inadequately address the specific toxicological profile of iron-rich PM10 and potentially adsorbed organics in these regions. To address this, comparative cytotoxicity experiments were carried out in-vitro using a variety of particulate standards, featuring other frequently studied metal-oxides, to help bridge in-vivo and epidemiological knowledge in the literature with in-vitro toxicological results. This also required the development of a novel framework to interpret physical and physiological characteristics of coarse, poorly soluble metal-oxide particles by conducting a critical systematic literature review.Analysis by the ChemCentre, WA was taken into account with relation to potentially absorbed organics and the influence of Polycyclic Aromatic Hydrocarbons (PAHs) investigated.The protocols developed by this project are applicable to high-throughput assessment of particulate matter generated by mining activity, having defined methodology to rapidly acquire sufficient quantities of PM10 for assays which can be multiplexed and the effects of particulate interference on assays minimised. This includes the integration of non-destructive sterilisation techniques, physical characterising by particle-sizing, particle solubility analysis and observation of particle morphology by electron microscopy and elemental analysis. Epithelial (A549), fibroblast (LL24) and monocyte differentiated macrophage (U937) cell lines were tested to build a picture of particulate effects on the major lung cell types in the lung parenchyma; the alveolar epithelium, connective tissue and immune system.The effect of lung fluid surfactants on cytotoxicity was determined, and advanced tissue culture methods were trialled to improve the differentiation between particles provided by the endpoints.ii The ore samples from the Mining Area C (MAC), Yandi and Newman Hub mines in the Pilbara region were found to have an effect comparable to the cytotoxicity of micron-size iron oxide standards and selected standards of titanium dioxide, silicon dioxide and carbon black, whereas copper oxide was found to have some ten-fold greater cytotoxicity across the cell lines. However cytotoxicity as a principle differentiator of toxicity was found to be insufficient in differentiating the physiological response to iron-rich PM10 and insoluble metal-oxide standards. At high concentrations the dose-response relationship plateaued at a maximum value for iron-oxide standards and iron ore samples in the A549 and LL2...

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Tl(I) and Tl(III) alter the expression of EGF‐dependent signals and cyclins required for pheochromocytoma (PC12) cell‐cycle resumption and progression

Pino

Verstraeten

2014

J of Applied Toxicology

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The effects of thallium [Tl(I) and Tl(III)] on the PC12 cell cycle were evaluated without (EGF(-)) or with (EGF(+)) media supplementation with epidermal growth factor (EGF). The following markers of cell-cycle phases were analyzed: cyclin D1 (G1 ); E2F-1, cyclin E and cytosolic p21 (G1 →S transition); nuclear PCNA and cyclin A (S); and cyclin B1 (G2). The amount of cells in each phase and the activation of the signaling cascade triggered by EGF were also analyzed. Tl(I) and Tl(III) (5-100 μM) caused dissimilar effects on PC12 cell proliferation. In EGF(-) cells, Tl(I) increased the expression of G1 →S transition markers and nuclear PCNA, without affecting cyclin A or cyclin B1. In addition to those, cyclin B1 was also increased in EGF(+) cells. In EGF(-) cells, Tl(III) increased the expression of cyclin D1, all the G1→S and S phase markers and cyclin B1. In EGF(+) cells, Tl(III) increased cyclin D1 expression and decreased all the markers of G1 →S transition and the S phase. Even when these cations did not induce the activation of EGF receptor (EGFR) in EGF(-) cells, they promoted the phosphorylation of ERK1/2 and Akt. In the presence of EGF, the cations anticipated EGFR phosphorylation without affecting the kinetics of EGF-dependent ERK1/2 and Akt phosphorylation. Altogether, results indicate that Tl(I) promoted cell proliferation in both EGF(-) and EGF(+) cells. In contrast, Tl(III) promoted the proliferation of EGF(-) cells but delayed it in EGF(+) cells, which may be related to the toxic effects of this cation in PC12 cells.

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In vitro assessment of cobalt oxide particle toxicity: Identifying and circumventing interference

Cited by 23 publications

References 55 publications

Nanotoxicology of Metal Oxide Nanoparticles

Nanotoxicology of Metal Oxide Nanoparticles

In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

Tl(I) and Tl(III) alter the expression of EGF‐dependent signals and cyclins required for pheochromocytoma (PC12) cell‐cycle resumption and progression

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