In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

“…The preservation of sperm membrane integrity and mitochondrial function during the freeze–thaw process is important for successful fertilization, but the commercial freezing media do not protect sperm from the loss of these functions [ 16 ]. The addition of cyto-protective factors in the freezing medium can prevent the deleterious effects of cryopreservation [ 92 ], because they can protect cells from the negative effects of ROS on sperm motility, mitochondrial activity, and DNA integrity [ 93 ].…”

“…Therefore, efficient and effective cryopreservation requires the addition of protective chemicals in the freezing media to increase cellular defenses and reduce ROS generation [ 10 , 14 , 15 ]. However, the commercial and homemade available freezing medium does not fully satisfy the requirements needed for the complete protection of sperm during the cryopreservation process [ 16 ]. Although Tris-egg yolk buffer is the most commonly used diluent for mammalian sperm, many studies show that the supplementation of anti-apoptotic factors, anti-oxidants, post-thaw enhancing chemicals, or novel cryo-protective agents is required to get good post-thaw results [ 1 , 5 , 17 , 18 ].…”

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

“…Soybean lecithin is an alternative for egg yolk and has been developed and used commercially for semen preservation [97]. Lecithin from soybean has been successfully used for semen cryopreservation [98]. Nowadays, researchers believe that extenders free from animal ingredients can decrease the risk of contamination transported by the animal source [93,99].…”

“…Nowadays, researchers believe that extenders free from animal ingredients can decrease the risk of contamination transported by the animal source [93,99]. Therefore, soybean lecithin can be used as an alternative to milk-or egg yolk-based extenders for semen cryopreservation in bulls [24,30,98], boars [100], rams [101], dogs [102], deer [103], and camels [104].…”

“…The prevailing phospholipids in most mammalian biological membranes can confer physical stability to sperm cells [105]. On the basis of the research results of Gamal et al [97] and Miguel-Jimenez et al [98], soybean lecithin-containing diluter is possibly the best semen extender in bulls. Furthermore, El-Keraby et al [106] found that the use of soybean-based extender increases sperm functional motility and reduces bacterial contamination in freezethawed bull semen.…”