In Vitro Assessment of Gentamicin Cytotoxicity on the Selected Mammalian Cell Line (Vero cells)

Abstract

The aim of this study was to evaluate the in vitro cytotoxicity of different concentrations (500-7500 μg/mL) of gentamicin -GENT (aminoglycoside antibiotic) on the selected mammalian cell line (Vero -cell line from African green monkey kidney). Analysis of the cell morphological changes was microscopically evaluated (magnification x 400). Quantification of Ca, Mg and total proteins was performed using spectrophotometry on device Rx Monza (Randox). Quantification of Na, K and Cl was performed on the automatic analyzer EasyLyte. The cell viability was assessed using the metabolic mitochondrial MTT test. Vero cells were able to survive at concentrations of 500 (89.21 %), 1000 (79.54 %) and 2000 μg/mL (34.59 %). We observed statistically significant decrease of vital cell content at concentrations of 2000, 4500, 7500 μg/mL against control group. Vero cell line slightly reacted to the presence of GENT but total proteins and mineral parameters were not significantly affected. Vero cells were highly sensitive to GENT with a significant decrease of viability at concentrations of 2000 and 4500 μg/mL (P < 0.001). Our data reveal that GENT has a significant cytotoxic and adverse effect on the cell viability.

Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium

“…It has been experimentally shown that some cell types in certain conditions may exhibit increased sensitivity to gentamicin and even end up in apoptosis . However, gentamicin is already the antibiotic used for human and animal treatment .…”

“…It is used for maintenance of cell lines, in order to prevent contamination and also to treat infected cell cultures. 84 It has been experimentally shown that some cell types in certain conditions may exhibit increased sensitivity to gentamicin 85 and even end up in apoptosis. 86 However, gentamicin is already the antibiotic used for human and animal treatment.…”

Nanostructured scaffold with biomimetic and antibacterial properties for wound healing produced by ‘green electrospinning’

“…RGD) in gelatin that mediate cell-substrate interactions [68] as well as the presence of interconnected pores and the rough surface created by the nanofibrous membrane structure that allows to support the proliferation and quicker regeneration of cells [8]. However, the addition of the antibacterial drugs could affect the cellular behavior as cytotoxic effect has been reported for certain amounts of the antibacterial agents [69,70]. In our work, GS loading amount does not affect the viability and morphology of neonatal normal human dermal fibroblasts in the presence of all the concentrations tested.…”

The effect of different sample collection methods on rabbit blood parameters

“…For analysis of cortisol DIALAB Elisa test kit (DIALAB Pruduktion and Vertrieb von chemish – technischen Produkten und Laborinstrumenten Gesellschaft m. b. H., Wiener Neudorf, Austria) was used. Mineral profile analysis was measured using commercial DiaSys (Diagnostic Systems GmbH, Holzheim, Germany) on the Randox RX Monza analyser (Crumlin, United Kingdom) ( Kováčik et al, 2017 , Kovacik et al, 2019 ).…”