In vitro Assessment of Helicobacter pylori Ureases Inhibition by Honey Fractions

Matongo, Fredrick; Nwodo, Uchechukwu U.

doi:10.1016/j.arcmed.2014.09.001

Cited by 24 publications

(24 citation statements)

References 26 publications

(16 reference statements)

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“…The main defense against this bacterium is to prevent it from adhering to the gastric mucosa by urease inhibition; urease inhibitors are particularly important in the treatment of gastric ulcers 11 . Propolis is a good pharmaceutical agent that has antibiotic, antitumor, antioxidant and anti-inflammatory properties.…”

Section: Resultsmentioning

confidence: 99%

“…Brucella agar (BA) with 7% horse blood and supplement (DENT) was used for H. pylori culture. Helicobacter pylori-selective supplement (DENT) containing vancomycin (10 mg/L), trimethroprim lactate (5 mg/L), cefsulodin (5 mg/L) and amphotericin B (5 mg/L), purchased from Oxoid (Hampshire, UK) 11 . Then, the H. pylori suspension was diluted to approximately 10 6 colony forming unit (cfu)/mL.…”

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

“…Simple susceptibility screening using the agar-well diffusion method as adapted earlier was performed 11 . Helicobacter pylori J99 was stored at À80 C in Brucella broth (BB; pH 7.0 ± 0.2, Merck) with 10% fetal calf serum supplemented with 20% glycerol.…”

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

“…The prevalence of antibiotic-resistant H. pylori strains and the side effects associated with the present chemotherapeutic approach driven the search for alternatives to currently available anti-H. pylori drugs, especially regarding safe and effective non-antibiotic agents 9 . Thus, the search for novel H. pylori urease inhibitors in natural products such as plant extracts 10 , honey 11,12 and propolis, with improved stability and low toxicity, is mandatory to improve the quality of life of human with H. pylori infection.…”

Section: Introductionmentioning

confidence: 99%

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Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

Baltaş¹,

Karaoğlu

Tarakçı

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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There is considerable interest in alternative approaches to inhibit Helicobacter pylori (H. pylori) and thus treat many stomach diseases. Propolis is a pharmaceutical mixture containing many natural bioactive substances. The aim of this study was to use propolis samples to treat H. pylori. The anti-H. pylori and anti-urease activities of 15 different ethanolic propolis extracts (EPEs) were tested. The total phenolic contents and total flavonoid contents of the EPE were also measured. The agar-well diffusion assay was carried out on H. pylori strain J99 and the inhibition zones were measured and compared with standards. All propolis extracts showed high inhibition of H. pylori J99, with inhibition diameters ranging from 31.0 to 47.0 mm. Helicobacter pylori urease inhibitory activity was measured using the phenol-hypochlorite assay; all EPEs showed significant inhibition against the enzyme, with inhibition concentrations (IC 50 ; mg/mL) ranging from 0.260 to 1.525 mg/mL. The degree of inhibition was related to the phenolic content of the EPE. In conclusion, propolis extract was found to be a good inhibitor that can be used in H. pylori treatment to improve human health.

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Section: Resultsmentioning

confidence: 99%

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

Baltaş¹,

Karaoğlu

Tarakçı

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…According to another aspect, the use of natural products, rather than chemical-based drugs, is desirable in the inhibition of these enzymes 3,20,21 . Recent studies have revealed that natural products are effective in the elimination of ROS and as enzyme inhibitors [22][23][24] . Considering these information, the objective of this study is to determine the antioxidant properties and inhibition effects of different types of honey (oak, chestnut and polyfloral honey) on urease and XO for the first time.…”

Section: Introductionmentioning

confidence: 99%

Honey as an apitherapic product: its inhibitory effect on urease and xanthine oxidase

Şahin

2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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The aim of this study was to evaluate new natural inhibitor sources for the enzymes urease and xanthine oxidase (XO). Chestnut, oak and polyfloral honey extracts were used to determine inhibition effects of both enzymes. In addition to investigate inhibition, the antioxidant capacities of these honeys were determined using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity assays. Due to their high phenolic content, chestnut and oak honeys are found to be a powerful source for inhibition of both enzymes. Especially, oak honeys were efficient for urease inhibition with 0.012-0.021 g/mL IC 50 values, and also chestnut honeys were powerful for XO inhibition with 0.028-0.039 g/mL IC 50 values. Regular daily consumption of these honeys can prevent gastric ulcers deriving from Helicobacter pylori and pathological disorders mediated by reactive oxygen species.

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Plant‐Derived Urease Inhibitors as Alternative Chemotherapeutic Agents

Hassan

Žemlička²

2016

Archiv der Pharmazie

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Inhibition of the metalloenzyme urease has important pharmacologic applications in the field of antiulcer and antigastric cancer agents. Urease is involved in many serious infections caused by Helicobacter pylori in the gastric tract as well as by Proteus and related species in the urinary tract. Although numerous studies have described several novel urease inhibitors (UIs) used for the treatment of gastric and urinary infections, all these compounds have exhibited severe side effects, toxicity, and instability. Therefore, to overcome such problems, it is necessary to search for new sources of UIs, such as natural products, that provide reduced side effects, low toxicity, greater stability, and bioavailability. As limited studies have been conducted on plant-derived UIs, this paper aims to highlight and summarize the most promising compounds isolated and identified from plants, such as terpenoids, phenolic compounds, alkaloids, and other substances with inhibitory activities against plant and bacterial ureases; these are in vitro and in vivo studies with an emphasis on structure-activity relationship studies and types of inhibition that show high and promising levels of anti-urease activity. This will aid medicinal chemists in the design and synthesis of novel and pharmacologically potent UIs useful for the development of antiulcer drugs.

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In vitro Assessment of Helicobacter pylori Ureases Inhibition by Honey Fractions

Cited by 24 publications

References 26 publications

Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

Honey as an apitherapic product: its inhibitory effect on urease and xanthine oxidase

Plant‐Derived Urease Inhibitors as Alternative Chemotherapeutic Agents

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