In vitro assessment of skin sensitization, photosensitization and phototoxicity potential of commercial glyphosate-containing formulations

Ávila, Renato Ivan de; Teixeira, Gabriel; Veloso, Danillo Fabrini Maciel Costa; Moreira, Larissa Cleres; Lima, Eliana Martins; Valadares, Marize Campos

doi:10.1016/j.tiv.2017.04.001

Cited by 27 publications

(18 citation statements)

References 43 publications

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“…In addition, glyphosate has been potentially correlated with disruption of glycine homeostasis (Pérez-Torres et al, 2017) and pathological conditions e.g., autism (Nevison, 2014), fatal chronic kidney disease (in regions with heavy metal contamination in water) (Jayasumana et al, 2014(Jayasumana et al, , 2015, bronchial inflammation (Kumar et al, 2014), cardiovascular diseases (Gress et al, 2015), and cancer (Paumgartten, 2017). Dermatology problems upon skin contact with glyphosate-based herbicides have also been reported (Amerio et al, 2004;Heras-Mendaza et al, 2008;de Ávila et al, 2017;Elsner et al, 2018). A recent clinical study on the severity of cardiovascular effects due to poisoning with glyphosatebased herbicides attributed differential cardiovascular toxicity to the salt form (ammonium or IPA) of glyphosate used in the formulation (Moon et al, 2018), which in light of the toxicity data of the formulants used, may not be fully justified, as could easily be caused by differences in the formulant.…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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“…In relation to the parameters investigated in this study, the hair dye ingredients and henna products tested yielded higher peptide depletion for cysteine than for lysine. This peptide reactivity profile has been related to chemicals and mixtures with skin sensitization potential . Furthermore, henna products containing PPD triggered greater peptide reactivity and intracellular IL‐18 production than the hair dye ingredients tested alone.…”

Section: Discussionmentioning

confidence: 95%

“…The mDPRA, an analogous assay to the direct peptide reactivity assay (DPRA), was carried out according to a protocol described previously, which was based on OECD No. 442C and DPRA DB‐ALM protocol No.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of in vitro testing strategies for hazard assessment of the skin sensitization potential of “real‐life” mixtures: The case of henna‐based hair‐colouring products containing p‐phenylenediamine

et al. 2019

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Background: Allergic contact dermatitis caused by henna-based hair-colouring products has been associated with adulteration of henna with p-phenylenediamine (PPD).Objectives: To develop a testing approach based on in vitro techniques that address key events within the skin sensitization adverse outcome pathway in order to evaluate the allergenic potential of hair-colouring products. Methods:The following in vitro assays were used to test the sensitizing capacity of hair dye ingredients: the micro-direct peptide reactivity assay (mDPRA); the HaCaT keratinocyte-associated interleukin (IL)-18 assay; the U937 cell line activation test (U-SENS)/IL-8 levels; the blood monocyte-derived dendritic cell test; and genomic allergen rapid detection (GARD skin). Those techniques with better human concordance were selected to evaluate the allergenic potential of 10 hair-colouring products.Results: In contrast to the information on the label, chromatographic analyses identified PPD in all products. The main henna biomarker, lawsone, was not detected in one of the 10 products. Among the techniques evaluated by testing hair dye ingredients, the mDPRA, the IL-18 assay, GARD skin and the U-SENS correlated better with human classification (concordances of 91.7%-100%) and were superior to the animal testing (concordance of 78.5%). Thus, these assays were used to evaluate haircolouring products, which were classified as skin sensitizers by the use of different two-of-three approaches.Conclusions: Our findings highlight the toxicological consequences of, and risks associated with, the undisclosed use of PPD in henna-based "natural" "real-life" products. K E Y W O R D Sallergic contact dermatitis, alternative methods, cosmetics, hair dyes, mixtures, skin sensitization

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“…Cell viability was measured by Neutral Red uptake methods. Phototox software (ZEBET, Germany) was used to determine the probability (p-value), Photo Irritation Factor (PIF) and Mean Photo Effect (MPE) of the test chemical being phototoxic according to previous study [ 14 ]. Probability was obtained by using PIF/MPE values and the computer-generated concentration-response data [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Exploration of alternative test methods to evaluate phototoxicity of ophthalmic agents by using Statens Seruminstitut Rabbit Cornea cell lines and 3D human reconstituted cornea models

2018

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Many chemicals have been reported to induce phototoxicity. The absorbance of light energy within the sunlight range is a common characteristic of phototoxicity. The 3T3 NRU phototoxicity test (PT) in 3T3 mouse skin fibroblasts has been used to identify the phototoxic potential induced by excited chemicals after exposure to ultra violet (UV). However, as phototoxicity may occur in ocular cells, it is necessary to develop a more suitable test for cornea-derived cells. In this study, we attempted to establish a new in vitro PT method in rabbit corneal cell lines (SIRC). We evaluated five ophthalmic agents, ciprofloxacin, levofloxacin, lomefloxacin, norfloxacin, and tetracycline, for their cytotoxic potential and in vitro phototoxicity. The results obtained using 3D human corneal models revealed that the UV-induced eye tissue toxicity by the test substances showed good correlation with those obtained using the in vitro phototoxicity test. However, the results from the 3D PT for ciprofloxacin, norfloxacin, and tetracycline in the 3D human cornea model were only partially comparable. Therefore, we suggest the SIRC cell line as a new phototoxicity test model; however, a sequential testing strategy, such as 3D PT, was also proposed to obtain relevant information for topical eye agents.

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In vitro assessment of skin sensitization, photosensitization and phototoxicity potential of commercial glyphosate-containing formulations

Cited by 27 publications

References 43 publications

Re-registration Challenges of Glyphosate in the European Union

Re-registration Challenges of Glyphosate in the European Union

Evaluation of in vitro testing strategies for hazard assessment of the skin sensitization potential of “real‐life” mixtures: The case of henna‐based hair‐colouring products containing p‐phenylenediamine

Exploration of alternative test methods to evaluate phototoxicity of ophthalmic agents by using Statens Seruminstitut Rabbit Cornea cell lines and 3D human reconstituted cornea models

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