In-vitro binding analysis and inhibitory effect of capsaicin on lipase

Wu, Di; Duan, Ran; Tang, Lan; Zhou, Di; Zeng, Zhen; Wu, Wen; Hu, Jie; Sun, Qiaomei

doi:10.1016/j.lwt.2021.112674

Cited by 32 publications

(15 citation statements)

References 40 publications

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“…The fluorescence quenching of aromatic amino acids is an efficient tool for determining the binding affinity and mechanism underlying ligand–protein interactions. − Indeed, when compared to alternative biophysical and biochemical methods for evaluating protein/ligand interaction, fluorescence quenching provides several advantages. , For instance, fluorescence intensity is directly proportional to the number of fluorophores present in a sample, which enables quantitative measurements. Also, fluorescence measurements have a very high sensitivity and may therefore be carried out on single molecules, allowing for the observation of biological mechanisms at the molecular level .…”

Section: Results and Discussionmentioning

confidence: 99%

Binding of the B-Raf Inhibitors Dabrafenib and Vemurafenib to Human Serum Albumin: A Biophysical and Molecular Simulation Study

et al. 2022

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Section: Results and Discussionmentioning

confidence: 99%

Binding of the B-Raf Inhibitors Dabrafenib and Vemurafenib to Human Serum Albumin: A Biophysical and Molecular Simulation Study

et al. 2022

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“…The potential of natural products for treating obesity is under exploration. Natural products be excellent alternative strategies for developing safe and more effective anti-obesity drugs [ 60 ]. A variety of natural products have been widely used in treating obesity including isolated pure natural compounds and crude extracts that can induce bodyweight reduction and prevent diet-induced obesity [ 1 ].…”

Section: Antioxidant Activities Of Chilis: An Overviewmentioning

confidence: 99%

Antioxidant, Anti-Obesity, Nutritional and Other Beneficial Effects of Different Chili Pepper: A Review

et al. 2022

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Fruits and vegetables are important components of a healthy diet. They are rich sources of vitamins and minerals, dietary fibre and a host of beneficial non-nutrient substances including plant sterols, flavonoids and other antioxidants. It has been reported that reduced intake of fruits and vegetables may increase the risk of non-communicable diseases (NCDs). Chili pepper, is a common and important spice used to enhance taste and nutrition. Over the years, reports have shown its potential as antioxidant and an anti-obesity agent. Obesity is a serious health concern as it may initiate other common chronic diseases. Due to the side effects of synthetic antioxidants and anti-obesity drugs, scientists are now focusing on natural products which produce similar effects to synthetic chemicals. This up-to-date review addresses this research gap and presents, in an accessible format, the nutritional, antioxidant and anti-obesity properties of different chili peppers. This review article serves as a reference guide for use of chili peppers as anti-obesity agents.

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“…If ΔG < 0, the reaction process is spontaneous. [30,31] Table 2 show that the value of ΔG at the corresponding temperature is negative, which indicates that MY or DMY binding to proteinase were spontaneous and enthalpically bound. According to the thermodynamic parameters ΔH, ΔG, and ΔS, a major role was played by hydrophobic forces in the interaction between DMY with Try and Lys and electrostatic force was the driving force in DMY binding to α-ChT.…”

Section: Thermodynamic Parameters and The Interaction Force Between D...mentioning

confidence: 99%

Investigation on the interaction between myricetin and dihydromyricetin with trypsin, α‐chymotrypsin, lysozyme by spectroscopy and molecular docking methods

Meng

Nan

Shi³

et al. 2022

Luminescence

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The interaction between myricetin and dihydromyricetin with trypsin, α-chymotrypsin and lysozyme was investigated using multispectral and molecular docking methods. The results of fluorescence quenching revealed that myricetin and dihydromyricetin could quench the intrinsic fluorescence of three different proteinases through a static quenching procedure. The binding constant and number of binding sites at different temperatures were measured. The thermodynamic parameters obtained at different temperatures showed van der Waals interactions and hydrogen bonds played the main roles in the interaction of myricetin with trypsin and lysozyme, hydrophobic force was dominant both in myricetin with α-chymotrypsin interaction and dihydromyricetin with trypsin and lysozyme interaction, as for the electrostatic forces, it was mainly the driving force in dihydromyricetin binding to α-chymotrypsin. There was non-radiative energy transfer between three proteinases and myricetin or dihydromyricetin with high probability. The microenvironment of trypsin, α-chymotrypsin and lysozyme is changed. The docking studies revealed that myricetin and dihydromyricetin entered the hydrophobic cavity of three proteinases and formed hydrogen bonds. The binding affinity of myricetin or dihydromyricetin is different with the trypsin, α-chymotrypsin and lysozyme due to the different molecular structure.

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In-vitro binding analysis and inhibitory effect of capsaicin on lipase

Cited by 32 publications

References 40 publications

Binding of the B-Raf Inhibitors Dabrafenib and Vemurafenib to Human Serum Albumin: A Biophysical and Molecular Simulation Study

Binding of the B-Raf Inhibitors Dabrafenib and Vemurafenib to Human Serum Albumin: A Biophysical and Molecular Simulation Study

Antioxidant, Anti-Obesity, Nutritional and Other Beneficial Effects of Different Chili Pepper: A Review

Investigation on the interaction between myricetin and dihydromyricetin with trypsin, α‐chymotrypsin, lysozyme by spectroscopy and molecular docking methods

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