In vitro bioassay investigations of suspected obesogen monosodium glutamate at the level of nuclear receptor binding and steroidogenesis

Shannon, Maeve; Wilson, Jodie; Xie, Yuling; Connolly, Lisa

doi:10.1016/j.toxlet.2018.10.021

Cited by 13 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animal models have the distinct and obvious disadvantage of not accurately replicating human physiology. However, animal models are an important and widely Bisphenol A (BPA) [97][98][99] Bisphenol S (BPS) [102] Bisphenol A diglycidyl ether (BADGE) [100] Triphenyltin [95] Dioctyl sodium sulfosuccinate (DOSS) [52] Geneistein & naringenin [103] Phthalate monoesters [32] 4-nonylphenol (NP) [101] Mono-ethylhexyl phthalate (MEHP) [50] Flavanone [104] Bixin, norbixin [105] Emodin [106] C2C12 (murine) Mono-ethylhexyl phthalate (MEHP) [50] HELA (human) Mono-ethylhexyl phthalate (MEHP) [50] Human embryonic kidney cells (HEK293C) Dioctyl sodium sulfosuccinate (DOSS) [52] HepG2 (human liver carcinoma cells) Bisphenol A (BPA), Bisphenol S (BPS) [102] Human adipose-derived stem cells (hASCs) Bisphenol A (BPA), Bisphenol A diglycidyl ether (BADGE) [102] Tributyltin (TBT) [44] Murine adipose derived stem cells (mASCs) Bisphenol A (BPA), Bisphenol A diglycidyl ether (BADGE) [102] Tributyltin (TBT) [44] Fao (murine hepatoma cells) Phthalate monoesters [32] COS (monkey kidney-derived cells) Bisphenol A (BPA), Bisphenol A diglycidyl ether (BADGE) [102] MBzP, MBuP [50] Mono-ethylhexyl phthalate (MEHP) [50,107] THP-1 macrophages (human) Psi-baptigenin, hesperidin [108] TARM-Luc (human, transfected T47-D epithelial cells) Monosodium glutamate (MSG) [109] KS483 (murine calvaria) Soy phytoestrogen genistein [110] C57BL/6 (murine-derived bone marrow stromal cells) Firemaster 550 [51] collagen embedded silk scaffolds Human embryonic-derived stem cells (hESCs) Tributyltin (TBT), Bisphenol A (BPA), Bisphenol S (BPS) [111] silk scaffolds Human adipose-derived stem cells (hASCs), Human umbilical vein endothelial cells (HUVECs)…”

Section: In Vivo Modelsmentioning

confidence: 99%

Mechanisms of action, chemical characteristics, and model systems of obesogens

et al. 2020

View full text Add to dashboard Cite

There is increasing evidence for the role of environmental endocrine disrupting contaminants, coined obesogens, in exacerbating the rising obesity epidemic. Obesogens can be found in everyday items ranging from pesticides to food packaging. Although research shows that obesogens can have effects on adipocyte size, phenotype, metabolic activity, and hormone levels, much remains unknown about these chemicals. This review will discuss what is currently known about the mechanisms of obesogens, including expression of the PPARs, hormone interference, and inflammation. Strategies for identifying obesogenic chemicals and their mechanisms through chemical characteristics and model systems will also be discussed. Ultimately, research should focus on improving models to discern precise mechanisms of obesogenic action and to test therapeutics targeting these mechanisms.

show abstract

Section: In Vivo Modelsmentioning

confidence: 99%

Mechanisms of action, chemical characteristics, and model systems of obesogens

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Although no studies have shown direct binding of glutamate ligand to steroid receptors, a few studies have suggested indirect interactions. First, in hippocampal neurons, androgen receptor activation changed how glutamate affected the intracellular calcium response (Foradori et al, 2007) and that glutamate had antagonistic effects on androgen receptor (Shannon et al, 2019). Furthermore, glutamate decarboxylase 65 converts glutamate to GABA, and it regulated androgen receptor binding to nuclear proteins in the context of prostate cancer (Gao et al, 2019).…”

Section: Potential Role Of Glutamate In Steroid-independent Female Sementioning

confidence: 99%

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Chiang

Park

2020

Front. Behav. Neurosci.

View full text Add to dashboard Cite

The survival of animal species predicates on the success of sexual reproduction. Neurotransmitters play an integral role in the expression of these sexual behaviors in the brain. Here, we review the role of glutamate in sexual behavior in rodents and non-rodent species for both males and females. These encompass the release of glutamate and correlations with glutamate receptor expression during sexual behavior. We then present the effects of glutamate on sexual behavior, as well as the effects of antagonists and agonists on different glutamate transporters and receptors. Following that, we discuss the potential role of glutamate on steroid-independent sexual behavior. Finally, we demonstrate the interaction of glutamate with other neurotransmitters to impact sexual behavior. These sexual behavior studies are crucial in the development of novel treatments of sexual dysfunction and in furthering our understanding of the complexity of sexual diversity. In the past decade, we have witnessed the burgeoning of novel techniques to study and manipulate neuron activity, to decode molecular events at the single-cell level, and to analyze behavioral data. They pose exciting avenues to gain further insight into future sexual behavior research. Taken together, this work conveys the essential role of glutamate in sexual behavior.

show abstract

“…Formazan crystals were solubilised in DMSO facilitated by agitation at 37 °C. Optical density was measured, as previously described (Shannon et al 2019), using a Sunrise spectrophotometer at 570 nm with a reference filter at 630 nm (TECAN, Switzerland). Metabolic activity was calculated as a percentage absorbance of the sample compared with the absorbance of the SC.…”

Section: Mtt Assaymentioning

confidence: 99%

Human-Based Exposure Levels of Perfluoroalkyl Acids May Induce Harmful Effects to Health by Disrupting Major Components of Androgen Receptor Signalling In Vitro

et al. 2019

Self Cite

View full text Add to dashboard Cite

Perfluoroalkyl acids (PFAAs) are detectable in human blood. PFAA exposure may contribute to androgen receptor (AR)related health effects such as prostate cancer (PCa). In Norway and Sweden, exposures to PFAAs and PCa are very real concerns. In vitro studies conventionally do not investigate PFAA-induced AR disruption at human blood-based concentrations, thus limiting application to human health. We aim to determine the endocrine disrupting activity of PFAAs based upon human exposure levels, on AR transactivation and translocation. PFAAs (PFOS, PFOA, PFNA, PFDA, PFHxS, and PFUnDA) were tested at concentrations ranging from 1/10 × to 500 × relative to human blood based upon the exposure levels observed in a Scandinavian population. Translocation was measured by high content analysis (HCA) and transactivation was measured by reporter gene assay (RGA). No agonist activity (translocation or transactivation) was detected for any PFAAs. In the presence of testosterone, AR translocation increased following exposure to PFOS 1/10 × and 100 ×, PFOA 1/10 ×, and PFNA 1 × and 500 × (P < 0.05). In the presence of testosterone, PFOS 500 × antagonised AR transactivation, whereas PFDA 500 × increased AR transactivation (P < 0.05). PFAAs may contribute to AR-related adverse health effects such as PCa. PFAAs can disrupt AR signalling via two major components: translocation and transactivation. PFAAs which disrupt one signalling component do not necessarily disrupt both. Therefore, to fully investigate the disruptive effect of human exposure-based contaminants on AR signalling, it is imperative to analyse multiple molecular components as not all compounds induce a disruptive effect at the same level of receptor signalling.

show abstract

In vitro bioassay investigations of suspected obesogen monosodium glutamate at the level of nuclear receptor binding and steroidogenesis

Cited by 13 publications

References 32 publications

Mechanisms of action, chemical characteristics, and model systems of obesogens

Mechanisms of action, chemical characteristics, and model systems of obesogens

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Human-Based Exposure Levels of Perfluoroalkyl Acids May Induce Harmful Effects to Health by Disrupting Major Components of Androgen Receptor Signalling In Vitro

Contact Info

Product

Resources

About