In vitro biological screening of a critically endangered medicinal plant, Atropa acuminata Royle Ex Lindl of north western Himalaya

Abstract

Atropa acuminata Royle Ex Lindl (Atropa acuminata) under tremendous threat of extinction in its natural habitat. However, the antimicrobial, antileishmanial and anticancer effects of the plant’s extracts have not been reported yet. In the current study, an attempt has been made to evaluate the pharmacological potential of this plant’s extracts against microbes, Leishmania and cancer. The roots, stems and leaves of Atropa acuminata were ground; then, seven different solvents were used alone and in different ratios to prepare crude extracts, which were screened for pharmacological effects. The aqueous, methanolic and ethanolic extracts of all parts carried a broad spectrum of anti-bacterial activities, while no significant activity was observed with combined solvents. Three types of cytotoxicity assays were performed, i.e., haemolytic, brine shrimp and protein kinase assays. The aqueous extract of all the parts showed significant haemolytic activity while n-hexane extracts of roots showed significant activity against brine shrimp. The acetone extracts strongly inhibited protein kinase while the methanolic extracts exhibited significant cytotoxic activity of roots and stem. The anti-leishmanial assays revealed that the methanolic extract of leaves and roots showed significant activity. These findings suggest that this plant could be a potential source of natural product based drugs.

Conjugation of Penicillin-G with Silver(I) Ions Expands Its Antimicrobial Activity against Gram Negative Bacteria

“…Evaluation of toxicity with brine shrimp assay: Brine shrimp assay was performed by a method previously described [38]. One gram cysts were initially hydrated in freshwater for one hour in a separating funnel or cone shaped container.…”

“…One gram cysts were initially hydrated in freshwater for one hour in a separating funnel or cone shaped container. Seawater was prepared by dissolving 17 g of sea salt in 500 mL of distilled water [38,39]. The cone was facilitated with good aeration for 48 h at room temperature and under continuous illumination.…”

In vitro and in vivo Assessment of Anti-Leishmanial Efficacy of Leaf, Fruit, and Fractions of Juniperus excelsa Against Axenic Amastigotes of Leishmania major and Topical Formulation in BALB/c Mice

“…Another research was conducted on the extract and fractions of Atropa acuminate, and ethyl acetate have shown anti-leishmanial effect (36). Study of the effect of methanol and chloroform extracts of Valeriana wallichii root showed positive activities against L. donovani and L. major promastigote and amastigote, while in the current study, the chloroform fraction of J. excelsa extract showed anti-L. major amastigotes activity (37).…”

Green Synthesis of Silver Atropa Acuminata Nanoparticles: Characterization and Anti-Diabetic Potential

“…After concentrating the filtrate, the extract was stored at a low temperature (5˚C) for further experiments. Atropa acuminata plant root extract lug numerous important phytochemical components that were qualitatively assessed in accordance with the literature review [35]. Alkaloids, phenolic chemicals, Green Synthesis of Silver Atropa Acuminata Nanoparticles: Characterization and Anti-Diabetic Potential ://jazindia.com https e online at: ilabl Ava -1104 -flavonoids and tannins were thus found by screening evidence, but no inference evidence for glycosides, terpenoids, proteins, or carbohydrates was found [36] [37].…”