In-vitro bipolar nano- and microsecond electro-pulse bursts for irreversible electroporation therapies

Sano, Michael B.; Arena, Christopher B.; DeWitt, Matthew R.; Saur, Dieter; Davalos, Rafael V.

doi:10.1016/j.bioelechem.2014.07.010

Cited by 101 publications

(62 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S10 and S11). It has been previously observed that H-FIRE treatment generates more homogenous lesions (2,24,(27)(28)(29)(30)(31) and in this article, we offer an explanation for that phenomenon.…”

Section: Figurementioning

confidence: 52%

Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation

Murovec

Sweeney

Latouche

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitulate multicellular environments in silico. Cells have been modeled as simple shapes in two dimensions as well as more complex geometries attempting to replicate realistic cell shapes. In this study, we describe a method for extracting realistic cell morphologies from fluorescence microscopy images to generate the piecewise continuous mesh used to develop a finite element model in two dimensions. The preelectroporation TMP induced in tightly packed cells is analyzed for two sets of pulse parameters inspired by clinical irreversible electroporation treatments. We show that high-frequency bipolar pulse trains are better, and more homogeneously raise the TMP of tightly packed cells to a simulated electroporation threshold than conventional irreversible electroporation pulse trains, at the expense of larger applied potentials. Our results demonstrate the viability of our method and emphasize the importance of considering multicellular effects in the numerical models used for studying the response of biological tissues exposed to electric fields.

show abstract

Section: Figurementioning

confidence: 52%

Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation

Murovec

Sweeney

Latouche

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…Specifically, a delay of 140 ns or less causes the nuclear TMP to double due to compounding effects from pulse falling edges and rising edges (49). In addition, metastatic cells with a larger nucleus-to-cytoplasm ratio can achieve even greater nuclear TMP.…”

Section: High-frequency Irreversible Electroporationmentioning

confidence: 99%

Maximizing Local Access to Therapeutic Deliveries in Glioblastoma. Part III: Irreversible Electroporation and High-Frequency Irreversible Electroporation for the Eradication of Glioblastoma

2017

Self Cite

View full text Add to dashboard Cite

Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. Approximately 9180 primary GBM tumors are diagnosed in the United States each year, in which median survival is up to 16 months. GBM eludes and resists typical cancer treatments due to the presence of infiltrative cells beyond the solid tumor margin, heterogeneity within the tumor microenvironment, and protection from the blood-brain barrier. Conventional treatments for GBM, such as surgical resection, radiotherapy, and chemotherapy, have shown limited efficacy;IRE and H-FIRE for the Eradication of GBM 374 therefore, alternate treatments are needed. Tumor chemoresistance and its proximity to critical structures make GBM a prime theoretical candidate for nonthermal ablation with irreversible electroporation (IRE) and high-frequency IRE (H-FIRE). IRE and H-FIRE are treatment modalities that utilize pulsed electric fields to permeabilize the cell membrane. Once the electric field magnitude exceeds a tissue-specific lethal threshold, cell death occurs. Benefits of IRE and H-FIRE therapy include, but are not limited to, the elimination of cytotoxic effects, sharp delineation from treated tissue and spared tissue, a nonthermal mechanism of ablation, and sparing of nerves and major blood vessels. Preclinical studies have confirmed the safety and efficacy of IRE and H-FIRE within their experimental scope. In this chapter, studies will be collected and information extrapolated to provide possible treatment regimens for use in high-grade gliomas, specifically in GBM.

show abstract

“…Currently, there are reports, which show that the efficacy of electroporation and the viability rate of the cells are dependent on the electric field strength, pulse duration, and pulse polarity [6,7]. However, dependent on the cell type, tissue, or buffer the parameters vary, which create a motivation for study of the permeabilization process in detail.…”

Section: Introductionmentioning

confidence: 98%

Analysis of Planar Circular Interdigitated Electrodes for Electroporation

Novickij

Tabasnikov

Smith

et al. 2015

IETE Technical Review

View full text Add to dashboard Cite

In this work, designs for planar, interdigitated circular electrode structures for cell membrane permeabilization procedures are analysed using finite element method analysis. The generated electric field intensity, homogeneity, and the Joule heating are evaluated. The optimal electrode configuration for successful transfection procedures is investigated. Based on the simulation results, an optimized design for the electrodes, suitable for the investigation of the permeabilization thresholds during electroporation is proposed. The Joule heating influence simulation results are validated experimentally.

show abstract

In-vitro bipolar nano- and microsecond electro-pulse bursts for irreversible electroporation therapies

Cited by 101 publications

References 64 publications

Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation

Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation

Maximizing Local Access to Therapeutic Deliveries in Glioblastoma. Part III: Irreversible Electroporation and High-Frequency Irreversible Electroporation for the Eradication of Glioblastoma

Analysis of Planar Circular Interdigitated Electrodes for Electroporation

Contact Info

Product

Resources

About