In vitro bone resorption is dependent on physiological concentrations of zinc

Chen, D.; Waite, Leonard C.; Pierce, William M.

doi:10.1007/bf02784036

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…Zinc is essential in the process of skeletal development, 1–50 μM zinc has been shown to inhibit osteoblast apoptosis and promote the proliferation and differentiation of osteoblasts, 98 and adding zinc (25–200 mg/dl) to the cultured chicken embryo tibia has been demonstrated to lead to a concentration‐dependent increase in tibial ALP activity and an increase in the level of bone formation, 99 physiological concentrations of zinc (25–200 mg/dl) have also been shown to increase bone resorption in tibia of chicken embryos. 100 Similarly, the effect of zinc on Ca 2+ in osteoblast‐related cells has not been exhibited, but it has been demonstrated that 10–30 μM zinc inhibited the increase in Ca 2+ concentration in BMMs induced by RANKL, and 30–100 μm zinc inhibited the CaN activity of BMMs. 101 …”

Section: Diverse Ca 2+ /Can/nfat Signalling Pathway Modulating Compounds Which Regulate Osteogenic Differentiationmentioning

confidence: 99%

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

et al. 2021

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The bone remodelling process is closely related to bone health. Osteoblasts and osteoclasts participate in the bone remodelling process under the regulation of various factors inside and outside. Excessive activation of osteoclasts or lack of function of osteoblasts will cause occurrence and development of multiple bone‐related diseases. Ca2+/Calcineurin/NFAT signalling pathway regulates the growth and development of many types of cells, such as cardiomyocyte differentiation, angiogenesis, chondrogenesis, myogenesis, bone development and regeneration, etc. Some evidences indicate that this signalling pathway plays an extremely important role in bone formation and bone pathophysiologic changes. This review discusses the role of Ca2+/Calcineurin/NFAT signalling pathway in the process of osteogenic differentiation, as well as the influence of regulating each component in this signalling pathway on the differentiation and function of osteoblasts, whereby the relationship between Ca2+/Calcineurin/NFAT signalling pathway and osteoblastogenesis could be deeper understood.

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Section: Diverse Ca 2+ /Can/nfat Signalling Pathway Modulating Compounds Which Regulate Osteogenic Differentiationmentioning

confidence: 99%

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

et al. 2021

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Extracellular and immunological actions of zinc

Rink

Gabriel

2001

Zinc Biochemistry, Physiology, and Homeostasis

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In Vitro effects of zinc on markers of bone formation

Chen

Waite

Pierce

1999

Biol Trace Elem Res

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Zinc deficiency is associated with a reduced rate of bone formation that can be corrected by supplementation of the deficient diet with adequate amounts of zinc. This study was conducted to examine the effects of zinc on bone cell parameters associated with bone formation. Tibiae were removed from 19-d-old chicken embryos and incubated for 48 h in Dulbecco's modified Eagle's medium supplemented with antibiotics, bovine serum albumin, and HEPES. The addition of zinc (25-200 g/dL) to tibial cultures resulted in a concentration-dependent increase in alkaline phosphatase activity, an increase in the incorporation of proline into bone protein and an increase in the post-translational oxidation of proline to peptidyl hydroxyproline. These effects of zinc were all diminished by the addition of 2,6-pyridine dicarboxylic acid, a chelator of zinc. The addition of either cycloheximide (10(-5)M), dactinomycin (10(-8)M), or hydroxyurea (10(-3)M) to tibial cultures also attenuated the effects of zinc. The effect of zinc on bone cell DNA synthesis was measured by following the incorporation of 3H-thymidine into DNA and by fluorometric measurement of cellular DNA content. These methods revealed that the addition of zinc to cultured tibiae resulted in a concentration-dependent increase in tibial DNA content and synthesis rate. The magnitude of the zinc-induced DNA increase was similar to the magnitude of the zinc-induced increases in alkaline phosphatase activity, proline incorporation, and hydroxyproline synthesis. Normalization of these latter responses to tibial DNA content yield data indicating that the effect of zinc on bone formation results from a zinc-induced increase in bone cell proliferation.

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In vitro bone resorption is dependent on physiological concentrations of zinc

Cited by 7 publications

References 11 publications

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

Extracellular and immunological actions of zinc

In Vitro effects of zinc on markers of bone formation

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In vitro bone resorption is dependent on physiological concentrations of zinc

Cited by 7 publications

References 11 publications

The role of Ca2+/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The role of Ca2+/Calcineurin/NFAT signalling pathway in osteoblastogenesis

Extracellular and immunological actions of zinc

In Vitro effects of zinc on markers of bone formation

Contact Info

Product

Resources

About

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis