In Vitro Callus Induction and Comparative Gc-MS Analysis of Methanolic Extracts of Callus and Leaf Samples of Ampelocissus Latifolia (Roxb.) Planch

Abstract

Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.

PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra)

“…Menurut Balamurugan (2011), gamma sitosterol dapat dipertimbangkan untuk menjadi obat anti diabetic. Dalam penelitian Anand, et al (2018) dalam ekstrak metanol kalus Ampelicissus latifolia teridentifikasi secara GCMS senyawa gamma sitosterol dengan luas area sebesar 9,19 %, senyawa ini memiliki aktivitas anti microbial, anti inflamatory, anti kanker, dan anti oksidan.…”

In Vitro Antimicrobial Activity, High-Performance Thin-Layer Chromatography, and Gas Chromatography-Mass Spectrometry Analysis of Ethanolic Seed Extract of Ficus Benghalensis Linn

“…India has a treasure of medicinal plants due to the rich diversity in its agro-climatic conditions. The curative properties of medicinal plants are mainly due to the presence of various secondary metabolites such as alkaloids, flavonoids, glycosides, and phenols present in them [5]. Secondary metabolites of plant origin appear to be one of the alternatives for the control of antibiotic-resistant human pathogens.…”