In Vitro Capacitation of Canine Spermatozoa.

Shimazu, Yoshiki; Yamada, Shuichi; Kawano, Yuko; Kawaji, H; Nakazawa, Masao; Maito, Kunihiko; Toyoda, Yutaka

doi:10.1262/jrd.38.67

Cited by 22 publications

(9 citation statements)

References 9 publications

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“…These changes, which prepare the spermatozoon for future gamete interactions, are collectively known as capacitation and they include destabilisation of the plasma membrane as well as changes to the expression of membrane surface antigens (Moore, 1995). In the dog it is also recognised that spermatozoa must also become capacitated prior to fertilization (Mahi andYanagimachi, 1976, 1978;Shimazu et al, 1992;Kawakami et al, 1993a) and in relation to this dog semen exhibits unusual features that may affect sperm capacitation. The dog lacks most accessory glands and seminal¯uid consists almost entirely of prostatic secretion.…”

Section: Introductionmentioning

confidence: 99%

Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

Brewis

Morton

Moore

et al. 2001

Molecular Reproduction Devel

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Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cytometric assay (one ejaculate from each dog examined; n = 6). No calcium flux was observed in the negative control treatments (RPMI medium or DMSO). Both heat-solubilized bitch ZP proteins and progesterone caused a similar response characterized by a gradual but marked influx of calcium ions which was sustained over 2 min. Acrosomal status was assessed by indirect immunofluorescence using a specific monoclonal antibody following 1 hr incubation for each treatment (four ejaculates from each dog examined; n = 24). The level of acrosomal exocytosis was very high for samples treated with ZP proteins (70.3 +/- 2.1%) and progesterone (84.6 +/- 1.5%) and was significantly different from the respective controls (P < 0.001). Interestingly the patterns of calcium flux in response to both ZP proteins and progesterone were in contrast to the situation in other species studied to date raising the possibility that the mechanism for triggering the acrosome reaction may be different in dog spermatozoa. In addition the high degree of progesterone-induced acrosomal exocytosis compared to other species raises the probability that the majority of dog spermatozoa are already undergoing the acrosome reaction before they reach the egg ZP.

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Section: Introductionmentioning

confidence: 99%

Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

Brewis

Morton

Moore

et al. 2001

Molecular Reproduction Devel

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“…This agrees with results published earlier regarding dog sperm capacitation. In the same way, the appearance of a very great percentage of head‐to‐head sperm agglutination of sperm, which also showed a characteristic tail undulatory movement, also indicates the attainment of a functional capacitation of these agglutinated cells (Shimazu et al. 1992; Rota et al.…”

Section: Discussionmentioning

confidence: 87%

In vitro Capacitation and Acrosome Reaction of Dog Spermatozoa can be Feasibly Attained in a Defined Medium Without Glucose

Albarracı́n

Mogas

Palomo

et al. 2004

Reprod Domestic Animals

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Incubation of dog spermatozoa in a medium without glucose and in the presence of lactate and pyruvate (l-CCM) for 4 h at 38.5 degrees C in a 5% CO(2) atmosphere induced in vitro capacitation of these cells. This was verified after the combined specific capacitation-like changes in percentages of viability and altered acrosomes, motility characteristics, sperm location of reactivity against Pisum sativum, Arachis hypogaea and Helix pomatia lectins and the tyrosine phosphorylation pattern. Furthermore, a feasible acrosome reaction (AR) was induced when spermatozoa incubated in l-CCM for 4 h were further co-incubated for 1 h with canine oocytes. This was demonstrated by AR-like changes in percentages of viability, altered acrosomes, motility characteristics and sperm location of reactivity against P. sativum, A. hypogaea and H. pomatia lectins. All these results clearly indicate that in vitro capacitation, and subsequent AR, can be feasibly achieved without the presence of sugars. This ability can be related to the specific characteristics of energy-metabolism regulation reported in dog spermatozoa.

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“…It is common for 60–80% of healthy‐appearing, germinal vesicle (GV) stage domestic cat (Godard et al, 2009), mouse (Marín Bivens et al, 2004), goat (Khatun et al, 2011), sheep (Shirazi et al, 2010), pig (Koike et al, 2010), and cow (Sirard et al, 1988) oocytes to transition from the GV stage to MII in vitro. Under these same laboratory conditions and with studies conducted by multiple, independent laboratories, however, only 20% of dog oocytes generally reach the MII stage (Mahi and Yanagimachi, 1976; Shimazu et al, 1992; Nickson et al, 1993; Yamada et al, 1993; Bolamba et al, 1998; Otoi et al, 2000; Luvoni et al, 2001; Saint‐Dizier et al, 2001; Kim et al, 2004; Songsasen and Wildt, 2007; Alhaider and Watson, 2009; Rodrigues et al, 2009; Silva et al, 2010; Evecen et al, 2011). One factor identified as a major influence on IVM success of the dog oocyte is the size of the donor follicle, where larger follicles provide oocytes with higher meiotic competency (Songsasen and Wildt, 2005).…”

Section: Introductionmentioning

confidence: 87%

The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation

Songsasen

Wesselowski

Carpenter

et al. 2011

Molecular Reproduction Devel

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We tested the hypothesis that meiotic competence of dog oocytes was tightly linked with donor follicle size and energy metabolism. Oocytes were recovered from small (< 1 mm diameter, n = 327), medium (1 – < 2 mm, n = 292) or large (≥ 2 mm, n = 102) follicles, cultured for 0, 24 or 48 h, assessed for glycolysis, glucose oxidation, pyruvate uptake, glutamine oxidation and then nuclear status. More oocytes (P < 0.05) from large follicles (37%) reached the metaphase II (MII) stage than from the small group (11%), with the medium size class being intermediate (18%; P > 0.05). Glycolytic rate increased (P < 0.05) as oocytes progressed from the germinal vesicle (GV) to MII stage. At 48 h of culture, oocytes completing nuclear maturation had higher (P < 0.05) glycolytic rate than those arrested at earlier stages. GV oocytes recovered from large follicle oocytes had higher (P < 0.05) metabolism than those from smaller counterparts at culture onset. MII oocytes from large follicles oxidized more (P < 0.05) glutamine than the same stage gametes recovered from smaller counterparts. In summary, larger size dog follicles contain a more metabolically-active oocyte with a greater chance of achieving nuclear maturation in vitro. These findings demonstrate a significant role of energy metabolism in promoting dog oocyte maturation, information that will be useful for improving culture systems for rescuing intraovarian genetic material.

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In Vitro Capacitation of Canine Spermatozoa.

Cited by 22 publications

References 9 publications

Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

In vitro Capacitation and Acrosome Reaction of Dog Spermatozoa can be Feasibly Attained in a Defined Medium Without Glucose

The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation

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