In Vitro Cell Culture Models of Hyperoxaluric States: Calcium Oxalate and Renal Epithelial Cell Interactions

Petrovic, Ana G.; Kizivat, Tomislav; Bilić-Ćurčić, Ines; Stoehr, Robert; Smolić, Martina

doi:10.3390/cryst11070735

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…Generally, urine has acidic pH, which is useful to prevent crystallization of stone-forming promoters by dissolving them into urine. 35 The measures of pH have shown alkaline urine in lithiatic control group caused by hyperoxaluric medium. Alkaline pH promotes the crystallization in urine and thus stone formation.…”

Section: Discussionmentioning

confidence: 99%

Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats

Chakit¹,

Boussekkour²,

Hessni³

et al. 2022

Pharmacogn J.

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In Morocco, Ziziphus lotus is commonly used as an urolithiatic agent in the traditional medicine. To confirm this effect, an aqueous extract of Ziziphus lotus (AEZL) has been studied in ethylene glycolinduced urolithiasis model of rats. 30 male rats were randomly divided into five groups of six animals each. Group I served as a vehicle control and received distilled water (0.5 ml/100 g p.o.). All remaining groups received calculi inducing treatment for 28 days, comprised of 0.75% v/v ethylene glycol with 1% w/v ammonium chloride in drinking water ad libitum for 3 days followed by only 0.75% v/v ethylene glycol for 25 days. Group II served as lithiatic control and received distilled water (0.5 ml/100 g p.o.). Group III served as curative treatment group and received AEZL at doses of 150 mg/kg from 14th day to 28th day. Group VI served as preventive treatment group and received AEZL at doses of 150 mg/kg from 1st day to 28th day. Group V served as therapeutic and received a drug "Cystone" at dose of 750 mg/kg from 14 th day to 28 th day. The extract treatment decreased the levels of oxalate and calcium in urine. Crystalluria analysis showed that untreated rats excreted large CaOx monohydrate and few dihydrate crystals while treated animals excreted mostly small CaOx dihydrate crystals. Significant similarity was observed between preventive and therapeutic anti-urolithiatic effect of AEZL and anti-urolithiatic effect of cystone (P<0.001). These results demonstrated that AEZL have an anti-urolithiatic effect with preventive and therapeutic treatments in this experimental condition.

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Section: Discussionmentioning

confidence: 99%

Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats

Chakit¹,

Boussekkour²,

Hessni³

et al. 2022

Pharmacogn J.

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“…Cell culture studies showed a correlation of oxalic acid concentration and reactive oxygen species (Moryama et al 2005). In a recent review, the interaction of calcium oxalate and renal epithelial cells in in vitro cell culture models has been discussed, and calcium oxalate has been reported to trigger epithelial-mesenchymal transition and carcinogenic features in renal cells (Petrovic et al 2021;Peerapen et al 2022). A typical daily intake of approximately 220 mg oxalate has been reported in the literature (Crivelli et al 2020), however, the contribution of the oral intake of oxalic acid for the formation of kidney stones is not yet fully understood.…”

Section: Toxicological and Commercial Aspectsmentioning

confidence: 99%

Upcycling of black currant pomace for the production of a fermented beverage with Wolfiporia cocos

et al. 2023

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Pomace as a side stream from black currant juice production is mostly discarded, even though it is rich in nutrients like protein, fiber, sugars, anthocyanins, polyphenols, and other secondary metabolites. Fungi from the division of Basidiomycota have a great enzymatic toolbox to recycle these complex mixtures of nutrients. In particular, the edible medicinal fungus Wolfiporia cocos has been described as a suitable biocatalyst to form pleasant aroma compounds in fermentation processes. Therefore, medium optimization, upscaling, and filtration were performed to produce a beverage based on black currant pomace fermented with W. cocos. A trained panel described the beverage as highly pleasant, reminiscent of honey, flowers and berries with a well-balanced sour and sweet taste. The flavor compounds linalool (citrus), geraniol (flowery), phenylacetic acid (honey), methyl phenylacetate (honey), eugenol (clove), and 2-phenylethanol (rose) were produced during fermentation and the concentrations exceeded their respective odor thresholds. The produced beverage was evaluated with 8.0 ± 1.4 from 10 for the question of whether panelists would buy the product. Fungal fermentation with the edible fungus W. cocos enabled the production of a highly pleasant beverage and additionally may reduce waste by using pomace and table sugar as sole ingredients.

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“…The chemical composition of urine determines the type of crystals, varies depending on the cause of nephrolithiasis, and is influenced by dietary habits, metabolic activity, and lifestyle [10]. Approximately 80% of kidney stones are calcium stones, predominantly calcium oxalate (CaOx) and calcium phosphate (CaP), whereas 9% are uric acid, 10% struvite, and 1% cystine stones [11,12]. The pathophysiological mechanism of KS includes nidation, growth, aggregation, retention, and results in crystal-renal epithelial cell interactions, leading to aberrant oxidative stress, inflammation, and injury to renal tissue [12,13].…”

Section: Introductionmentioning

confidence: 99%

A Mechanistic Insight into Beneficial Effects of Polyphenols in the Prevention and Treatment of Nephrolithiasis: Evidence from Recent In Vitro Studies

Hefer,

Huskic,

Petrovic

et al. 2023

Crystals

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Nephrolithiasis is a pathological condition characterized by the formation of solid crystals in the kidneys or other parts of urinary tract. Kidney stones are a serious public health issue and financial burden for health care system, as well as a painful and uncomfortable condition for patients, resulting in renal tissue injury in severe cases. Dietary habits, low fluid and high salt intake predominantly, contribute to the development of kidney stones. Current research suggests that polyphenols have a protective effect in the pathogenesis of kidney stones. Polyphenols are a group of naturally occurring compounds found in plant-based foods such as fruits, vegetables, tea, and coffee. In this review, we explore mechanisms underlying the beneficial effects of polyphenols, such as oxidative stress reduction and modulation of inflammatory pathways, in various in vitro models of nephrolithiasis. Additionally, certain polyphenols, such as catechins found in green tea, have been shown to inhibit the formation and growth of kidney stones in animal studies. This review highlights the antioxidant and anti-inflammatory effects, as well as the inhibition of crystal formation, as results of polyphenol treatment in vitro. Further research is required to determine the specific effects of polyphenols on kidney stone formation in humans; however, current knowledge implicates that incorporating a variety of polyphenol-rich foods into the diet may be a beneficial strategy for individuals at risk of developing nephrolithiasis.

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In Vitro Cell Culture Models of Hyperoxaluric States: Calcium Oxalate and Renal Epithelial Cell Interactions

Cited by 7 publications

References 61 publications

Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats

Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats

Upcycling of black currant pomace for the production of a fermented beverage with Wolfiporia cocos

A Mechanistic Insight into Beneficial Effects of Polyphenols in the Prevention and Treatment of Nephrolithiasis: Evidence from Recent In Vitro Studies

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