In Vitro Characterization of a Recombinant AHL-Lactonase from Bacillus cereus Isolated from a Striped Catfish (Pangasianodon hypophthalmus) Pond

Tình, Nguyễn Thị; Dung, Nguyen Viet; Trung, Cao Thanh; Thuy, Van Thi

doi:10.1007/s12088-013-0415-y

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…In the present study, a plasmid harboring the DNA sequence encoding segments of AiiA and MagR was constructed to facilitate expression of MagR‐tagged AiiA in E. coli host. The sequence of protein AiiA was based on AHL from Bacillus cereus isolated from striped catfish ( Pangasianodon hypophthalmus ) pond (Tinh, Dung, Trung, & Thuy, ). The sequence of protein MagR was based on magnetic receptor, isoform A from Drosophila melanogaster (Cyranoski, ).…”

Section: Resultsmentioning

confidence: 99%

Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Wang

Liu

et al. 2019

MicrobiologyOpen

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Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3O4‐SiO2 iron beads and was confirmed via SDS‐PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6‐HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.

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Section: Resultsmentioning

confidence: 99%

Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Wang

Liu

et al. 2019

MicrobiologyOpen

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“…The quest for novel bioactive agents which are efficient and stable under extreme conditions is incessant [35,36]. Against this backdrop, the solidity of antibiofilm activity displayed by CAB isolates was tested at high temperature treatment.…”

Section: Resultsmentioning

confidence: 99%

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Padmavathi

Pandian

2014

Indian J Microbiol

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Coral Associated Bacteria (CAB) (N = 22) isolated from the mucus of the coral Acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. Six CAB (U7, U9, U10, U13, U14, and U16) were found to produce biosurfactants and were identified by 16S ribosomal RNA gene sequencing as Providencia rettgeri, Psychrobacter sp., Bacillus flexus, Bacillus anthracis, Psychrobacter sp., and Bacillus pumilus respectively. Their cell surface hydrophobicity was determined by Microbial adhesion to hydrocarbon assay and the biosurfactants produced were extracted and characterized by Fourier Transform Infrared spectroscopy. Since the biosurfactants are known for their surface modifying capabilities, antibiofilm activity of positive isolates was evaluated against biofilm forming Pseudomonas aeruginosa ATCC10145. Stability of the active principle exhibiting antibiofilm activity was tested through various temperature treatments ranging from 60 to 100°C and Proteinase K treatment. CAB isolates U7 and U9 exhibited stable antibiofilm activity even after exposure to higher temperatures which is promising for the development of novel antifouling agents for diverse industrial applications. Further, this is the first report on biosurfactant production by a coral symbiont.

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“…AHLs can be degraded through the enzymatic activity of lactonases, acylases, or oxi-reductases. To date, mainly lactonases, but also one oxidoreductase and one putative acylase, have been described in Bacillus species [42,[44][45][46]. When an AHL-lactonase is present, cleavage of the homoserine lactone ring of the AHL molecule occurs.…”

Section: Discussionmentioning

confidence: 99%

Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection

et al. 2021

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The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. Bacillus spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 Bacillus spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested Bacillus spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely Aeromonas spp., Vibrio spp., Photobacterium damselae, Edwardsiela tarda, and Shigella sonnei. The results revealed that A. veronii and E. tarda produce QS molecules that are detectable by the Chr. violaceum biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as B. subtilis, B. vezelensis, and B. pumilus, significantly reduced the pathogenicity of E. tarda in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three Bacillus spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.

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In Vitro Characterization of a Recombinant AHL-Lactonase from Bacillus cereus Isolated from a Striped Catfish (Pangasianodon hypophthalmus) Pond

Cited by 11 publications

References 21 publications

Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection

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