In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

Fujisawa, Sotaro; Shiro, Motoo; Takehara, Masaki; Aoyama, Julia; Morita, Ayu; Isezaki, Masayoshi; Win, Shwe Yee; Ariizumi, Takuma; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Maekawa, Naoya; Okagawa, Tomohiro; Ichii, Osamu; Konnai, Satoru; Ohashi, Kazuhiko

doi:10.1016/j.vaccine.2021.08.104

Cited by 8 publications

(16 citation statements)

References 27 publications

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“…PRM samples were prepared as described previously [4,5,19]. PRMs of mixed developmental stages and sexes were obtained from an egg-laying farm in Japan.…”

Section: Prm Samplesmentioning

confidence: 99%

“…RNA isolation and cDNA synthesis from PRM samples were performed as described previously [4,5]. Each collected PRM sample was suspended in 600 µL of Buffer RLT Plus (RNeasy Plus Mini Kit) (Qiagen, Hilden, Germany) and homogenized thoroughly using a 1.5-mL Homogenization Pestle for a 1.5-mL microcentrifuge tube (Scientific Specialties, Inc., Lodi, CA, USA).…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

“…The production of specific antibodies against Dg-Cys-his was examined by Western blotting, as described previously [4,5]. Purified Dg-Cys-his was separated using a 14% SDS-polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Merck Millipore, Burlington, MA, USA).…”

Section: Western Blottingmentioning

confidence: 99%

“…To assess the effects of the "cocktail vaccine", plasmas from chickens immunized with copper transporter 1-like molecule (Dg-Ctr1 [4]) or adipocyte plasma membrane-associated protein-like molecule (Dg-APMAP [5]) were mixed with an equal volume of immune plasmas of Dg-Cys. As a control, equal volumes of plasmas from chickens immunized with PBS, as described above, were mixed with plasmas prepared in our previous studies [4,5]. Blood feeding was conducted as described above using adult PRMs, and the SR was monitored for a 10-day period.…”

Section: Evaluation Of the Efficacy Of The "Cocktail Vaccine"mentioning

confidence: 99%

“…Since continuous use of chemical acaricides, which are commonly used to prevent PRM infestations, could cause various problems such as the selection of drug-resistant PRMs and the contamination of products with acaricides, an alternative control method is needed. Over the past few years, vaccination has been in the spotlight as a novel strategy for the control of ectoparasites, including PRMs, and several studies have examined antigen candidates for their efficacies [1][2][3][4][5]. However, adequate anti-PRM efficacy has not been reported through practical applications in the field [6].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens

et al. 2021

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Poultry red mite (PRM; Dermanyssus gallinae) is a hazardous, blood-sucking ectoparasite of birds that constitutes a threat to poultry farming worldwide. Acaricides, commonly used in poultry farms to prevent PRMs, are not effective because of the rapid emergence of acaricide-resistant PRMs. However, vaccination may be a promising strategy to control PRM. We identified a novel cystatin-like molecule in PRMs: Dg-Cys. Dg-Cys mRNA expression was detected in the midgut and ovaries, in all stages of life. The PRM nymphs that were artificially fed with the plasma from chickens that were immunized with Dg-Cys in vitro had a significantly reduced reproductive capacity and survival rate. Moreover, combination of Dg-Cys with other antigen candidates, like copper transporter 1 or adipocyte plasma membrane-associated protein, enhanced vaccine efficacies. vaccination and its application as an antigen for cocktail vaccines could be an effective strategy to reduce the damage caused by PRMs in poultry farming.

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“…PRM samples were prepared as described previously [4,5,19]. PRMs of mixed developmental stages and sexes were obtained from an egg-laying farm in Japan.…”

Section: Prm Samplesmentioning

confidence: 99%

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Section: Western Blottingmentioning

confidence: 99%

Section: Evaluation Of the Efficacy Of The "Cocktail Vaccine"mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens

et al. 2021

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Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species

et al. 2023

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Infestation with poultry red mites (PRM, Dermanyssus gallinae) causes anemia, reduced egg production, and death in serious cases, resulting in significant economic losses to the poultry industry. As a novel strategy for controlling PRMs, vaccine approaches have been focused upon and several candidate vaccine antigens against PRMs have been reported. Tropical (TFM, Ornithonyssus bursa) and northern (NFM, Ornithonyssus sylviarum) fowl mites are also hematophagous and cause poultry industry problems similar to those caused by PRM. Therefore, ideal antigens for anti-PRM vaccines are molecules that cross-react with TFMs and NFMs, producing pesticidal effects similar to those against PRMs. In this study, to investigate the potential feasibility of developing vaccines with broad efficacy across mite species, we identified and characterized cysteine proteases (CPs) of TFMs and NFMs, which were previously reported to be effective vaccine antigens of PRMs. The open reading frames of CPs from TFMs and NFMs had the same sequences, which was 73.0% similar to that of PRMs. Phylogenetic analysis revealed that the CPs of TFMs and NFMs clustered in the same clade as CPs of PRMs. To assess protein functionality, we generated recombinant peptidase domains of CPs (rCP-PDs), revealing all rCP-PDs showed CP-like activities. Importantly, the plasma obtained from chickens immunized with each rCP-PD cross-reacted with rCP-PDs of different mites. Finally, all immune plasma of rCP-PDs reduced the survival rate of PRMs, even when the plasma was collected from chickens immunized with rCP-PDs derived from TFM and NFM. Therefore, CP antigen is a promising, broadly efficacious vaccine candidate against different avian mites.

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Poultry Mites: Ubiquitous, Spreading, and Still a Growing Threat

et al. 2022

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In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

Cited by 8 publications

References 27 publications

Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens

Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens

Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species

Poultry Mites: Ubiquitous, Spreading, and Still a Growing Threat

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