In vitro chemotherapy sensitivity patterns of human colon carcinoma continuous cell lines

Abstract

The cells obtained from four established human colon carcinoma cell lines were cultured in soft agar in the presence of eight chemotherapeutic agents that have been used clinically in patients with metastatic colorectal carcinoma. 5-Fluorouracil, mitomycin C, adriamycin, and cis-platinum were highly cytotoxic in vitro at the concentrations employed. These findings were in marked contrast to in vitro chemotherapy sensitivity patterns previously reported by us for a large series of primary human colorectal carcinomas cultured in soft agar. The possible reasons behind these differences are discussed.

In vitro Sensitivity of Immature Human Mast Cells to Chemotherapeutic Agents

“…), and cytosine arabinoside (Cytosar-U, 1.0, 0.1 and 0.01 pg/ml, Upjohn Co., Kalamazoo, Mich.). These concentrations had been utilized in previous studies by other authors [8,9] or spanned the range of reported achievable in vivo serum or plasma concentrations [10][11][12][13][14][15][16][17][18][19][20][21].…”