In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

Abstract

Bioactive compounds and phenolic compounds are viable alternatives to antibiotics in recurrent urinary tract infections. This study aimed to use a natural functional product, based on the bioactive compounds’ composition, to inhibit the uropathogenic strains of Escherichia coli. E. coli ATCC 25922 was used to characterize the IVCM (new in vitro catheterization model). As support for reducing bacterial proliferation, the cytotoxicity against a strain of Candida albicans was also determined (over 75% at 1 mg/mL). The results were correlated with the analysis of the distribution of biologically active compounds (trans-ferulic acid-268.44 ± 0.001 mg/100 g extract and an equal quantity of Trans-p-coumaric acid and rosmarinic acid). A pronounced inhibitory effect against the uropathogenic strain E. coli 317 (4 log copy no./mL after 72 h) was determined. The results showed a targeted response to the product for tested bacterial strains. The importance of research resulted from the easy and fast characterization of the functional product with antimicrobial effect against uropathogenic strains of E. coli. This study demonstrated that the proposed in vitro model was a valuable tool for assessing urinary tract infections with E. coli.

Antimicrobial and Antibiofilm Activity of the Probiotic Strain Streptococcus salivarius K12 against Oral Potential Pathogens

“…In this study, a standard assay in microtiter plates was used to examine in vitro antimicrobial and antibiofilm activity, similar to previous studies [29,30]. In addition to the standard methodology, new innovative approaches to in vitro testing of antimicrobial activity with inhibition of biofilm formation are also available, such as the dental implant in vitro model stimulating biofilm formation [31], the in vitro lab catheterization model [32], and biofilm formation in real time using automated microfluidics [28].…”

Exploring the Efficacy of Extracts for Cosmetic Creams: In Vivo and In Vitro Assessments

“…The determinations were conducted according to previous methods in published studies [12,13,15]. According to a previous method, a 3 mL aliquot of the sample was weighed and then diluted with 3 mL of methanol.…”

Functional Compounds from Banana Peel Used to Decrease Oxidative Stress Effects

“…The correlation of the antimicrobial effect with the antioxidant and cytotoxic capacity demonstrated a new valorization possibility, for example, in controlling recurrent infections with MDR pathogenic strains. The target could be the functional product that could control microbial proliferation in hospitals [23].…”