In vitro Comparison of Disk Diffusion and Agar Dilution Antibiotic Susceptibility Test Methods for Neisseria gonorrhoeae

Castillo, Marta C. de; Saab, Olga A. de; Nader, Olga M. de; Holgado, A. Pesce De Ruiz

doi:10.1590/s0074-02761998000400019

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Antibacterial activity of tested bacterial strains was assayed using all plant extracts by agar diffusion technique (de Castillo et al 1998). Antibacterial activity was observed as inhibition zone on Petri plates containing nutrient agar.…”

Section: Determination Of Antibacterial Activitymentioning

confidence: 99%

Evaluation of antibacterial activity of different mangrove plant extracts

2006

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Antibacterial activity of mature leaves, tender leaves and bark extracts of Avicennia marina, Avicennia officinalis and Bruguiera sexangula were evaluated using Soxhelt extraction method. Petroleum ether, chloroform, ethyl acetate and ethanol were used as solvents in order to get the plant extracts. The antibacterial activity was screened by using agar diffusion technique against pathogenic bacteria species of Staphylococcus sp. (from urine), Proteus sp. (from a wound), Escherichia coli (from infected blood), Shigella sp. (from a wound) and Pseudomonas sp. (from a wound). The length of inhibition zone was measured in millimeters from the edge of the well to the edge of the inhibition zone. Twelve different plant extracts in A. marina, A. officinalis and B. sexangula exhibited different degree of growth inhibition against tested bacterial strains. Mature leaf extracts of A. marina and tender leaf extracts of A. officinalis in ethyl acetate exhibited promising antibacterial activity than other plant extracts. All plant extracts in ethyl acetate showed strong inhibition compared to other extracts on all tested bacterial strains. Among all bacterial strains, Pseudomonas sp. and E. coli showed considerable growth inhibition against almost all plant extracts. Mature leaf extract of only A. marina was used for further investigations since a large amount of tender leaves of A. officinalis was not readily available. Charcoal treated mature leaf extracts of A. marina showed more inhibition for all tested bacterial strains than untreated plant extracts. Combinations of mature leaf extracts of A. marina in different solvents failed to exhibit synergistic activity against tested bacterial strains. Most combinations showed antagonistic effect against Proteus sp., E. coli and Shigella sp. Antibacterial activity of plant extracts of A. marina gradually declined during 4 months after extraction, for all tested bacterial strains. Components of mature leaf extracts of A. marina in chloroform, ethyl acetate and ethanol were separated by Analytical Thin Layer Chromatography (ATLC) when the eluents were hexane and diethyl ether. Isolated components in Preparative Thin Layer Chromatography (PTLC) exhibited moderate antibacterial activity against Pseudomonas sp., Shigella sp. and E. coli. Separated components did not show any antibacterial activity against Proteus sp. and Staphylococcus sp. Phytochemical screening revealed that mature leaf of A. marina contained alkaloids, steroids, triterpenoids and flavonoids.

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Section: Determination Of Antibacterial Activitymentioning

confidence: 99%

Evaluation of antibacterial activity of different mangrove plant extracts

2006

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“…Wells (6 mm dia) were gently made on the agar solidified medium with a sterilized cork borer, and the wells were later filled with 65 μl (50 mg/mL) of the plant extracts. The plates were left for incubation in an incubator at 27 °C for 24 h. The diameters of the inhibition zone were recorded using HiMedia Antibiotic Scale [29] . For negative control, DMSO without extracts was used, whereas antibiotics (viz., azithromycin and roxithromycin) and antifungals (viz., clotrimazole and fluconazole) were used as positive controls.…”

Section: Materials and Methodologymentioning

confidence: 99%

“…The plates were left for incubation in an incubator at 27 °C for 24 h. The diameters of the inhibition zone were recorded using HiMedia Antibiotic Scale. [29] For negative control, DMSO without extracts was used, whereas antibiotics (viz., azithromycin and roxithromycin) and antifungals (viz., clotrimazole and fluconazole) were used as positive controls.…”

Section: Antimicrobial Activity Assaymentioning

confidence: 99%

Antimicrobial and Antioxidant Property of a True Mangrove Rhizophora apiculata Bl.

Acharya

Jali

Pradhan

et al. 2023

Chemistry & Biodiversity

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Mangroves are abundant in bioactive natural substances that fight off pathogenic diseases. Different parts of R. apiculata, an abundant mangrove found in Bhitarkanika National Park, India were extracted with methanol and a mixture of solvents methanol/ethanol/chloroform (60 : 20 : 20) to evaluate their antimicrobial properties. The combination solvent extract of bark had the highest zone of inhibition (ZOI) of 18.62 mm against Pseudomonas aeruginosa and a ZOI of 17.41 mm against Streptococcus mitis. Bark extracts had the highest DPPH (43 %) and FRAP (96 %) activities. The combination solvent bark extract of R. apiculata had the highest ZOI of 20.42 mm (lowest MIC of 2.12 μg/ml) against Candida albicans and ZOI of 15.33 mm (MIC of 3.02 μg/mL) against Penicillium chrysogenum. Combination bark extracts of R. apiculata contained flavanols than methanolic extracts. The crude extract of R. apiculata bark made with a mixture of solvents containing more active ingredients could be used in novel drug formulation.

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“…So many researchers studied correlation between zones of inhibition and diff usion tests [3 5]. and diffusion tests [3][4][5]…”

Section: Introductionmentioning

confidence: 99%

Antibiotics Sensitivity Against Staphylococcus aureus and Escherichia coli using Industrially Manufactured Sensitivity Disc

Khanam*

2023

J Biomed Res Environ Sci

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Staphylococcus aureus a gram-positive bacteria cause diseases through direct destruction and invasion of tissue by the production of toxins and Escherichia coli a gram-negative bacteria causes a variety of diseases. Testing of antibacterial susceptibility plays a major role in antibacterial treatment. Their are some factors which affects the results are quality, type and structure of a disks. This study was carried out with the aim to determine the Zone of inhibition of antibiotics against Staphylococcus aureus and Escherichia coli using industrially manufactured sensitivity disc. In positive test against Staphylococcus aureus the zone of inhibition was highest in antibiotic streptomycin (10mm/dm) and lowest in Amoxil (0mm/dm). In negative test against Staphylococcus aureus the zone of inhibition was highest in antibiotic Reflacine (10mm/dm) and lowest in Amplicin (2mm/dm). In positive test against Escherichia coli the zone of inhibition was highest in antibiotic Ciproflox (7mm/dm) and lowest in Amoxil (2mm/dm). There is no effect on antibiotic Norfloxacin, Erythromycin, Levofloxacin and Ampiclox. In negative test against Escherichia coli the zone of inhibition was highest in antibiotic Streptomycin (12mm/dm) and lowest in Tarivid (2mm/dm

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In vitro Comparison of Disk Diffusion and Agar Dilution Antibiotic Susceptibility Test Methods for Neisseria gonorrhoeae

Cited by 6 publications

References 16 publications

Evaluation of antibacterial activity of different mangrove plant extracts

Evaluation of antibacterial activity of different mangrove plant extracts

Antimicrobial and Antioxidant Property of a True Mangrove Rhizophora apiculata Bl.

Antibiotics Sensitivity Against Staphylococcus aureus and Escherichia coli using Industrially Manufactured Sensitivity Disc

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