In vitro competition between calcium phytate and the soluble fraction of rat small intestine contents for cadmium, copper and zinc

Wise, Alan; Gilburt, D.J.

doi:10.1016/0378-4274(82)90104-7

Cited by 23 publications

(7 citation statements)

References 3 publications

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“…Ultimately, the possible effect of phytate, and in turn, of phytase, on copper availability remains debatable. It may be speculated that copper is better protected from the chelation by phytates than zinc because of its high affinity for some other chelating agents, namely amino acids ( Wise and Gilburt, 1982), that would keep it soluble in the intestinal lumen ( Mills, 1985).…”

Section: Discussionmentioning

confidence: 99%

Replacement of zinc sulphate by microbial phytase for piglets given a maize-soya-bean meal diet

2005

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Forty-eight pigs, weaned at 27 days of age at an average body weight of 7·55 kg were used in a 19-day experiment to investigate the influence of microbial phytase on zinc utilization and to calculate equivalency values of zinc as sulphate for microbial phytase. Eight experimental diets were formulated : a maize-soya-bean meal basal diet containing 30 mg of zinc per kg supplemented with 10, 25, 40 or 100 mg of zinc from sulphate ( ZnSO 4, . 7H 2 O) per kg or with 100, 250, 500 or 750 units (U) of microbial phytase (3-phytase from Aspergillus niger, Natuphos ®) per kg. The dietary supplies of calcium and phosphorus were adjusted accounting for the release of these elements by microbial phytase. The copper concentration in the diets was 11 mg/kg. Pigs were given the basal diet for a 7-day adjustment period prior to the 19-day experimental period. At the end of the experiment, bone ash, phosphorus and calcium concentrations as well as plasma and liver copper concentrations were independent of the diet ( P > 0·10). The zinc status of piglets was assessed through plasma alkaline phosphatase activity (APA) and zinc concentration, bone zinc concentration and liver zinc concentration. Plasma zinc, plasma APA and bone zinc increased linearly ( P < 0·001) and quadratically ( P < 0·01, P < 0·001 and P < 0·001, respectively) with zinc added. These parameters also increased linearly ( P < 0·001) and quadratically ( P < 0·05, P < 0·001 and P < 0·05, respectively) with phytase added. Liver zinc increased quadratically ( P < 0·05) with zinc added and tended to increase linearly with phytase added ( P = 0·077). Linear and non-linear response equations of indicators of zinc status to zinc added and phytase added were developed and used to calculate zinc equivalency values of phytase. Non-linear models were linear plateau models for zinc added and exponential models for phytase added. Plasma APA, plasma zinc and bone zinc were maximized when zinc added reached 43, 54 and 56 mg/kg of diet, respectively. The mean function of equivalency of zinc as sulphate (Zn, mg/kg of diet) for microbial phytase ( Phyt, U per kg of diet) was Zn = 49·9-58·3 e -0·00233 Phyt . From this equation it is calculated that 250, 500, and 750 U of 3-phytase from Aspergillus niger can avoid the addition of 17, 32 and 40 mg of zinc as sulphate in a piglet diet. Zinc ingested and, in turn, zinc excreted, may be proportionately reduced by almost 0·30 by replacing 30 mg of zinc as sulphate by 500 U of phytase as Natuphos ® in a piglet maize and soya-bean meal diet formulated to contain 100 mg of zinc per kg.

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Section: Discussionmentioning

confidence: 99%

Replacement of zinc sulphate by microbial phytase for piglets given a maize-soya-bean meal diet

2005

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“…Turnlund, et al [37] observed that dietary supplementation of sodium phytate or a-cellulose has no influence on the copper absorption. There is a notion that in the presence of excess calcium, copper may be precipitated with phytate [38]. Davis, et al [39] reported that dietary supplementation of crude soybean proteins decreased the bioavailability and deficiency of copper in broilers.…”

Section: Copper Absorptionmentioning

confidence: 99%

Does Dietary Fiber Affect the Levels of Nutritional Components after Feed Formulation?

Adams

Sello

Qin

et al. 2018

Fibers

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Studies on dietary fiber and nutrient bioavailability have gained an increasing interest in both human and animal nutrition. Questions are increasingly being asked regarding the faith of nutrient components such as proteins, minerals, vitamins, and lipids after feed formulation. The aim of this review is to evaluate the evidence with the perspective of fiber usage in feed formulation. The consumption of dietary fiber may affect the absorption of nutrients in different ways. The physicochemical factors of dietary fiber, such as fermentation, bulking ability, binding ability, viscosity and gel formation, water-holding capacity and solubility affect nutrient absorption. The dietary fiber intake influences the different methods in which nutrients are absorbed. The increase in the total fiber content of the diet may delay the glycemic response. Soluble fiber decreased blood glucose content whereas purified insoluble fiber has a little or no effect on the blood glucose levels after a meal. Dietary fiber and prebiotics influence the host animal well-being by regulating blood glucose or insulin levels, stool bulking effects, increasing the acidity of the gut, constructive synthesis of short chain fatty acids (SCFAs), decreasing intestinal transit time, stimulating the growth of intestinal microbes, and increasing blood parameters. Previous studies suggest that fiber affects the bioavailability of nutrients, and maintains the host wellness.

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“…In rats fed on high-Ca diets, phytate hydrolysis appears to depend mainly on caecal bacterial phytase and this has been demonstrated by both in vivo (Wise & Gilburt, 1 9 8 2~; and in vitro techniques (Wise & Gilburt, 1987). In vitro studies have also shown that various chelating agents like histidine are able to desorb some Zn from the Zn-Ca-phytate precipitate, and the capacity of the rat's intestinal contents to desorb Zn has also been shown to depend on dietary protein (Wise & Gilburt, 1982b). Similarly, in vivo studies have concluded that increased dietary protein is able to reduce the detrimental effect of dietary phytate on Zn absorption in rats and humans (Sandstrom et al 1980;Davies, 1982).…”

mentioning

confidence: 99%

Binding of zinc and calcium to inositol phosphates (phytate) in vitro

Simpson

Wise

1990

Br J Nutr

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Inositol compounds with three to five phosphate groups (IP3-IP5) were produced by hydrolysis of phytate (inositol hexaphosphate, IP6) and their binding affinities for calcium and zinc investigated at neutral pH with relative concentrations that had been found in a range of students' meals. Zn solubility was negligible a t many of these concentrations, with less Zn bound to precipitates of Ca-TP6 than Ca-IP5. The capacity to precipitate Zn a t these ratios fell between IP5 and IP3. Zn was partially desorbed by soluble chelators (histidine and picolinate), especially when it had been adsorbed to preformed Ca-IP precipitates. A lower proportion of Zn was accessible to soluble chelators from Ca-IP4 than the other compounds. IP3-TP4 were hydrolysed by phytase more readily than IP5-IP6.

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In vitro competition between calcium phytate and the soluble fraction of rat small intestine contents for cadmium, copper and zinc

Cited by 23 publications

References 3 publications

Replacement of zinc sulphate by microbial phytase for piglets given a maize-soya-bean meal diet

Replacement of zinc sulphate by microbial phytase for piglets given a maize-soya-bean meal diet

Does Dietary Fiber Affect the Levels of Nutritional Components after Feed Formulation?

Binding of zinc and calcium to inositol phosphates (phytate) in vitro

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