In vitro confocal micro-PIV measurements of blood flow in a square microchannel: The effect of the haematocrit on instantaneous velocity profiles

Lima, Rui; Wada, Satoshi; Takeda, Motohiro; Tsubota, Ken Ichi; Yamaguchi, Takami

doi:10.1016/j.jbiomech.2007.01.012

Cited by 88 publications

(70 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The scanning time was decreased considerably by the Nipkow disk and a rotating disk with microlenses to focus the laser. Typical full-field scanning rates were 50 Hz (Lima et al 2006), 120 Hz (Park et al 2004), 200 Hz (Lima et al 2007) up to 4,800 Hz (Kinoshita et al 2007). Liebling et al (2006) used a slit instead of pinholes to record a whole line of the sample at once.…”

Section: Confocal Scanning Microscopymentioning

confidence: 99%

“…Confocal scanning microscopy shows better performance especially for larger volume fractions ([0.45%) of particles in the fluid. Scanning confocal microscopy was successfully applied to microscopic flows of evaporating droplets (Kinoshita et al 2007), biological cell flows (Lima et al 2006(Lima et al , 2007, and capillary flows (Park et al 2004). Ichiyanag et al (2007) combined laser-induced fluorescence (LIF) for the measurement of the pH-value with 2D lPIV.…”

Section: Confocal Scanning Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Cierpka

Kähler

2011

J Vis

131

View full text Add to dashboard Cite

The reliable measurement of the 3D3C velocity field in microfluidic devices becomes more and more important for future optimization and developments for lab-on-a-chip applications or point-of-care medical diagnosis systems. In the past years, different particle-based imaging methods, such as confocal scanning microscopy, holography, stereoscopic and tomographic imaging or approaches based on defocused particle images or optical aberrations have been developed and applied successfully to measure velocity fields in microfluidic systems. The benefits and drawbacks of these methods will be discussed in detail as the proper understanding of the measurement principle is essential to select the most appropriate technique for a desired measurement application. Once an imaging method is chosen, the velocity can be estimated by correlation-based methods or tracking approaches. The advantages and disadvantages of both methods and the importance of image preprocessing will also be discussed in detail.

show abstract

Section: Confocal Scanning Microscopymentioning

confidence: 99%

Section: Confocal Scanning Microscopymentioning

confidence: 99%

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Cierpka

Kähler

2011

J Vis

131

View full text Add to dashboard Cite

show abstract

“…The most plausible explanation relies on the formation of a cell-free plasma layer near the wall, with red blood cells (RBCs) tending to migrate toward the center of the microtube. Extensive research ensued on the flow properties of blood in both dilute and concentrated suspensions using several measuring techniques such as double-slit photometry 7,8 , video microscopy and image analysis [4][5][6]9 , laser-Doppler anemometry 10,11 , and particle-measuring methods [12][13][14][15] . Although extensive studies have been conducted on the microhemodynamic behavior of single RBCs in dilute suspensions, their behavior in concentrated suspensions remains poorly understood, in part due to technical limitations.…”

Section: Introductionmentioning

confidence: 99%

“…Recent progress in confocal microscopy 22,23 have led to a new technique known as confocal microparticle image velocimetry (PIV) 14,15,[24][25][26][27] . This method combines conventional PIV with a spinning disk confocal microscope (SDCM) to obtain in-focus images with an optical thickness of less than 1 µm (optical sectioning effect).…”

Section: Introductionmentioning

confidence: 99%

“…The superior spatial resolution and improved contrast and definition allow the measurement of several microrheology phenomena in concentrated suspensions of RBCs. Recently, our confocal system was combined with a cross-correlation technique, to obtain detailed information on the in vitro velocity profiles of blood with hematocrits (Hcts) up to 17% 14 . However, velocity field errors resulting from trace particles necessitate combining the developed confocal system with a single particle tracking (SPT) method to characterize higher Hct level phenomena.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Measurement of Individual Red Blood Cell Motions Under High Hematocrit Conditions Using a Confocal Micro-PTV System

et al. 2009

Self Cite

View full text Add to dashboard Cite

Developments in optical experimental techniques have helped in elucidating how blood flows through microvessels. Although initial developments were encouraging, studies on the flow properties of blood in microcirculation have been limited by several technical factors, such as poor spatial resolution and difficulty obtaining quantitative detailed measurements at such small scales. Recent advances in computing, microscopy, and digital image processing techniques have made it possible to combine a particle tracking velocimetry (PTV) system with a confocal microscope. We document the development of a confocal micro-PTV measurement system for capturing the dynamic flow behavior of red blood cells (RBCs) in concentrated suspensions. Measurements were performed at several depths through 100-µm glass capillaries. The confocal micro-PTV system was able to detect both translational and rotational motions of individual RBCs flowing in concentrated suspensions. Our results provide evidence that RBCs in dilute suspensions (3% hematocrit) tended to follow approximately linear trajectories, whereas RBCs in concentrated suspensions (20% hematocrit) exhibited transversal displacements of about 2% from the original path. Direct and quantitative measurements indicated that the plasma layer appeared to enhance the fluctuations in RBC trajectories owing to decreased obstruction in transversal movements caused by other RBCs. Using optical sectioning and subsequent image contrast and resolution enhancement, the system provides previously unobtainable information on the motion of RBCs, including the trajectories of two or more RBCs interacting in the same focal plane and RBC dispersion coefficients in different focal planes.

show abstract

Automatic tracking of labeled red blood cells in microchannels

Pinho

Lima

Pereira

et al. 2012

Numer Methods Biomed Eng

Self Cite

View full text Add to dashboard Cite

The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- μm glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland-Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels.

show abstract

In vitro confocal micro-PIV measurements of blood flow in a square microchannel: The effect of the haematocrit on instantaneous velocity profiles

Abstract: A confocal microparticle image velocimetry (micro-PIV) system was used to obtain detailed information on the velocity profiles for the flow of pure water (PW) and in vitro blood (haematocrit up to 17%)

Cited by 88 publications

References 23 publications

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Measurement of Individual Red Blood Cell Motions Under High Hematocrit Conditions Using a Confocal Micro-PTV System

Automatic tracking of labeled red blood cells in microchannels

Contact Info

Product

Resources

About