In vitro conservation of Passiflora —A review

Pacheco, Graziela Drociunas; Simão, Mariela J.; Vianna, Marco Tadeu Gomes; Garcia, Renata; Vieira, Maria Lúcia Carneiro; Mansur, Elisabeth

doi:10.1016/j.scienta.2016.09.004

Cited by 38 publications

(44 citation statements)

References 78 publications

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“…Micropropagation studies in Passiflora started in 1966 (Nakayama, 1966) from the culture of nodal segments of an important ornamental species, P. caerulea. Since then, a growing number of studies describing in vitro techniques applied to the genus have been published for commercial and wild species with ornamental and phamaceutic propreties (Drew, 1997;Otoni et al, 2013;Ozarowski and Thiem, 2013;Rathod et al, 2014;Pacheco et al, 2016;Rocha et al, 2018;Vieira et al, 2018).…”

Section: Micropropagation Of Wild Passiflora Spp With Ornamental Potmentioning

confidence: 99%

“…The collecting and conserving the diversity of Passiflora germplasm is of great importance, no only as a source of genes and natural products, but also for its ecological value (Pacheco et al, 2016). Traditionally, Passiflora genetic resources has been conserved in germplasm banks through ex situ conservation strategies (Bernacci et al, 2005;Cerqueira-Silva et al, 2016).…”

Section: Conservation and Cryopreservationmentioning

confidence: 99%

“…These flowers are found in the most diverse forms, sizes, and colors and have a distinguished scent (Montero et al, 2013). In the view of the recognized economic importance of the wild and commercial passion fruit species several tissue culture and biotechnological techniques have been applied to the genus (Silva et al, 2009;Otoni et al, 2013;Ozarowski and Thiem 2013;Pacheco et al, 2016;Carvalho et al, 2017;Faria et al, 2018). The present review proposes to summarize the results achieved thus far in the biotechnology of wild Passiflora species with ornamental potential.…”

Section: Introductionmentioning

confidence: 99%

“…Passiflora species are commonly used for their fruits and derivatives, medicinal plant and as a source of oils for cosmetic industry (Pacheco et al, 2016;Cerqueira-Silva et al, 2014). The use of passion flowers as an ornamental plant is due their beauty and exuberance.…”

Section: Introductionmentioning

confidence: 99%

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Tissue culture and biotechnological techniques applied to passion fruit with ornamental potential: an overview

Mikosvki

Silva

Souza

et al. 2019

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The ornamental flower sector has growing over the past years worldwide with potential for further expansion. Among the ornamental plants, Passiflora species have been gaining ground in the market, mainly in European and North American countries. However, the market aiming the use of these species in ornamentation is still poorly explored. The inclusion of passion flower in the list of ornamental plants is related to the peculiar characteristics of the flower as it is complex structure, capacity of flowering all year long and also by the abundance and exuberance of the leaves, which in many species adds an ornamental value. Among the biotechnological tools for the production of ornamental plants, tissue culture has been outstanding in the cloning of elite genotypes, with high phytosanitary quality and large scale production. In addition, it offers possibilities of producing new varieties with characteristics peculiar to the market of ornamental plants. The diversity of wild Passiflora opens perspectives to the conservation, market and production of ornamental Passiflora cultivars.

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Section: Micropropagation Of Wild Passiflora Spp With Ornamental Potmentioning

confidence: 99%

Section: Conservation and Cryopreservationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tissue culture and biotechnological techniques applied to passion fruit with ornamental potential: an overview

Mikosvki

Silva

Souza

et al. 2019

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“…In vitro conservation is based on tissue culture techniques and is a complementary strategy to conventional preservation methods. These biotechnological techniques have various advantages, such as production of plants free of pathogens and high multiplication rates under controlled aseptic conditions, reducing the requirements for quarantine and the labor and space necessary for conservation (Pacheco et al, 2016). Besides these advantages, it facilitates the availability of germplasm for use in genetic improvement programs (Arrigoni-Blank et al, 2014), and can be realized by changes in the culture conditions aiming to decelerate or totally suppress the growth of cells, tissues and organs, for the purpose of maximizing the interval between subcultures, or extending a single culture indefinitely.…”

Section: Introductionmentioning

confidence: 99%

In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

et al. 2019

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Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

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Seed morphoanatomy of the genus PassifloraL. (Passifloraceae) by scanning electron microscopy and light microscopy

Silva

Souza

Junghans

et al. 2022

Microscopy Res & Technique

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Morphoanatomical analysis of seeds contributes to knowledge of the development of seedlings and identification of species, as well as supporting conservation studies. The conservation of the species belonging to the Passiflora genus is crucial due to of the threats to the genetic resources of these species. Thus, the objective of this study was to morphoanatomically characterize Passiflora seeds, verify possible injuries to the tissues after cryopreservation and thus contribute to the conservation strategies of the species of this genus. Initially, seeds of Passiflora coccinea, P. edulis, P. gibertii, P. maliformis, P. morifolia, P. setacea, P. suberosa, and P. tenuifila collected from the Passion Fruit Active Germplasm Bank of the Embrapa Cassava and Fruits research unit (Embrapa Mandioca e Fruticultura) were analyzed. Then, their length, width and thickness, shape of the base and tip, and ornamentations present on the body and edge of the seeds were evaluated. The seeds of the species were placed in cryotubes and immersed in liquid nitrogen to assess possible cryoinjuries. The tegument and tissues of the seeds were examined by scanning electron microscopy. The seeds had varied biometric data, with average values of 4.63 mm for length, 3.28 mm for width, and 1.51 mm for thickness. Six ornamentation types were observed: reticulate for the species P. coccinea; finely reticulate for P. edulis; foveolate reticulate for P. gibertii and P. setacea; alveolate reticulate for P. maliformis and P. tenuifila; coarsely reticulate for P. morifolia; and falsifoveolate reticulate for P. suberosa. Some seeds suffered tegument cracks due to the freezing in liquid nitrogen, but without physiological damages to the embryo and endosperm. The cryopreservation of the seeds in the presence of the tegument significantly reduced the cryoinjuries caused to the embryo. Cryopreservation can be promising for long-term conservation of passion fruit seeds.

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In vitro conservation of Passiflora —A review

Cited by 38 publications

References 78 publications

Tissue culture and biotechnological techniques applied to passion fruit with ornamental potential: an overview

Tissue culture and biotechnological techniques applied to passion fruit with ornamental potential: an overview

In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

Seed morphoanatomy of the genus PassifloraL. (Passifloraceae) by scanning electron microscopy and light microscopy

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