In Vitro Conservation of Plant Genetic Resources

Withers, Lyndsey A.; Engelmann, Florent

doi:10.1201/9781420049275.ch4

Cited by 62 publications

(55 citation statements)

References 95 publications

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“…& Alina Trejgell trejgell@umk.pl material for a few months to several years (depending on the species) under tissue culture conditions, requiring only infrequent subculturing (Rao 2004). Slow growth of in vitro cultures can be achieved by reducing the osmotic potential of the storage medium, lowering incubation temperature or altering medium composition and photon flux density (Withers and Engelmann 1997). The most important factor in the slow growth culture is temperature reduction, often with a simultaneous decrease in light intensity or even culture in the dark, which limits shoot growth from callus induction and proliferation of the base of the shoots.…”

Section: Introductionmentioning

confidence: 99%

In vitro slow growth storage of Senecio macrophyllus shoots

2015

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Advances in biotechnology, especially in the field of in vitro culture techniques, led to the development of procedures that can be used as an excellent tool in plant conservation. The present study describes in vitro conservation of Senecio macrophyllus by slow-growth storage. Various sugar treatments, concentration of abscisic acid (ABA), light intensity and type of containers were tested. Viability and proliferation rate of shoots were evaluated 4 weeks after regrowth. The results obtained showed that polycarbonate boxes were a better type of containers for storage of S. macrophyllus shoots than glass vessels. Light negatively affected culture viability, multiplication rate and rooting response. In the case of Senecio macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on medium without ABA. Shoots of S. macrophyllus were effectively stored for 6 months. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization.

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Section: Introductionmentioning

confidence: 99%

In vitro slow growth storage of Senecio macrophyllus shoots

2015

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“…Temperatures of 0-5ºC are used with cold tolerant species, but, temperatures of 15º-20ºC are recommended for tropical species. Growth can also be limited by modifying the culture medium, mainly by sugar reducing and changes concentration mineral elements and by covering explants with a layer of liquid medium or mineral oil for reduction of oxygen level in cultures (Withers and Engelmann 1997). According to Withers and Engelmann (1997) successful regeneration and propagation of stable seedlings from in-vitro cultures are pre-requisites for any in-vitro conservation and techniques for clonal multiplication are well established for a number of species and slow growth procedures have been developed for a wide range of species; however, they are routinely used only for a few species including banana, potato, sweet potato, cassava, yam, Allium spp and temperate tree species for conservation.…”

Section: Conservation Biotechnology By Bk Joshimentioning

confidence: 99%

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

Joshi

2017

J. Nep. Agric. Res. Counc.

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Agricultural biodiversity is the basis of human life and food security. Nepal with 577 cultivated species possesses huge diversity at varietal as well as landrace levels. In most agricultural crops the rapid genetic erosion due to several reasons is a common phenomenon. Thus, considering the importance of agricultural biodiversity declared by Convention on Biological Diversity for sustainable food production, National Agriculture Genetic Resources Center (NAGRC) has been established for conservation and sustainable utilization of agricultural biodiversity. This paper thus delineates the application of biotechnological tools adopted by NAGRC for effective and efficient conservation and use of agricultural plant genetic resources (APGRs). Among the adopted technologies, tissue bank using shoot tip culture of vegetatively propagating and recalcitrant crops eg potato, sugarcane, banana, sweet potato, etc are in function. Under the molecular marker technology, currently random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers have been used for developing DNA profiles, identifying duplicates in the collections, assessing genetic diversity and screening accessions against economic traits. DNA bank has also been created for storing DNA of indigenous crops and these DNA can be accessed for research and study. Genotypic database has been developed for chayote, finger millet, wheat and maize for identification and selection of the accessions.

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“…Since, last 40 years, CIP has been contributed to developing tissue culture techniques for conserving potato germplasm (Withers et al, 1997).It is the most prominent and efficient way for distributing clonal materials. It ensures the availability of planting material any time and made possible the eradication of virus through meristem culture.…”

Section: Introductionmentioning

confidence: 99%

In vitro conservation of exotic potato genotypes through different Incubated temperatures, aerophilic and micro-aerophilic conditions

Ali¹,

Erum²,

Nouroz³

et al. 2016

Int. J. Biodivers. Conserv.

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The present study was carried out to study the in vitro conservation of potato genotypes at different temperatures and aerophilic and micro aerophilic conditions. A total of 31 genotypes were conserved at different incubated temperature ranging from 10 to 25°C. At lower temperature (10°C) plant growth was slowest as compared to plants incubated at high temperature (25°C). The results revealed that aerophilic condition was optimum for the growth of all potato genotypes. Data were collected on plant height, number of roots and number of nodes. Maximum plant height, highest number of roots and number of nodes were observed in all genotypes grown at 25°C. In vitro microareophillic condition of the plant growth was very slow but conservation was maximum. It was concluded from the present investigation that low temperature and micro-aerophilic condition is best for in vitro conservation of International Potato Center (CIP) germplasm which can increase the period between sub culturing.

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In Vitro Conservation of Plant Genetic Resources

Cited by 62 publications

References 95 publications

In vitro slow growth storage of Senecio macrophyllus shoots

In vitro slow growth storage of Senecio macrophyllus shoots

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

In vitro conservation of exotic potato genotypes through different Incubated temperatures, aerophilic and micro-aerophilic conditions

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