In Vitro Corneal Tomography of Donor Cornea Using Anterior Segment OCT

Janunts, Edgar; Langenbucher, Achim; Seitz, Berthold

doi:10.1097/ico.0000000000000761

Cited by 21 publications

(16 citation statements)

References 11 publications

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“…Measurements of the central corneal thickness were performed with 3-dimensional crosssectional imaging before and after decellularization as described earlier 55 .…”

Section: Quantification Of Tissue Swellingmentioning

confidence: 99%

A Decellularized Human Corneal Scaffold for Anterior Corneal Surface Reconstruction

Polisetti,

Schmid,

Schlötzer-Schrehardt

et al. 2020

SSRN Journal

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Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects.

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“…Measurements of the central corneal thickness were performed with 3-dimensional crosssectional imaging before and after decellularization as described earlier 55 .…”

Section: Quantification Of Tissue Swellingmentioning

confidence: 99%

A Decellularized Human Corneal Scaffold for Anterior Corneal Surface Reconstruction

Polisetti,

Schmid,

Schlötzer-Schrehardt

et al. 2020

SSRN Journal

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“…OCT can also be used to image donor corneas, to reduce the risk of transplanting donor tissue with undiagnosed pathologies such as keratoconus or corneas that have previously undergone laser refractive procedures which were not reported by donors’ families …”

Section: Corneal Transplantsmentioning

confidence: 99%

Optical coherence tomography imaging in keratoconus

Yip

Chan

2019

Clinical and Experimental Optometry

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Optical coherence tomography (OCT) is being increasingly used as a tool in the diagnosis and management of keratoconus. While elevation-based topography remains essential, there is an expanding role for cross-sectional OCT imaging in the diagnosis of the disease. Images and measurements of corneal thickness, and in particular, epithelial thickness, may be important in diagnosing early cases, and following procedures such as intrastromal corneal ring segments, corneal transplants and corneal collagen cross-linking.

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“…OCT technology allows sterile, direct noncontact tomographic imaging of corneal donor OCT -Applications in Ophthalmology tissue with the donor tissue placed within the culture flask. First attempts have been presented by Neubauer and Priglinger [45,46] and the method has been enhanced with different devices and methods by Janunts et al [47] (CASIA SS-1000, Tomey Corp., Nagoya, Japan) and Damian et al…”

Section: Screening Of Corneal Donor Tissuementioning

confidence: 99%

Imaging the Cornea, Anterior Chamber, and Lens in Corneal and Refractive Surgery

Eppig¹,

Mäurer²,

Daas³

et al. 2018

OCT - Applications in Ophthalmology

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Anterior segment OCT (AS-OCT) is an optical and noncontact imaging technology, which has numerous fields of application in the imaging of the cornea, anterior chamber, and the lens. In this chapter, we will present some of the application fields of AS-OCT in corneal, cataract, and refractive surgery. We will emphasize the potential of AS-OCT by several clinical examples including corneal imaging (keratoconus, keratoplasty, and refractive surgery) and intraocular lens imaging after refractive surgery. AS-OCT shows special potential for corneal imaging in case of corneal edema and for postoperative control after Descemet's membrane endothelial keratoplasty (DMEK). The postoperative follow-up of a posterior chamber Collamer lens'ses vault and measuring the anterior chamber angle could be identified as another promising field of application for AS-OCT.

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In Vitro Corneal Tomography of Donor Cornea Using Anterior Segment OCT

Abstract: Donor corneal examination using sterile viewing chambers was found to be suitable as a pre-keratoplasty advanced screening routine. A proof of concept was demonstrated, which could identify both irregular corneas and those affected by keratoconus.

Cited by 21 publications

References 11 publications

A Decellularized Human Corneal Scaffold for Anterior Corneal Surface Reconstruction

A Decellularized Human Corneal Scaffold for Anterior Corneal Surface Reconstruction

Optical coherence tomography imaging in keratoconus

Imaging the Cornea, Anterior Chamber, and Lens in Corneal and Refractive Surgery

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